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Localisation of basic fibroblast growth factor in cholesteatoma matrix: an immunochemical study

Published online by Cambridge University Press:  27 February 2019

M A Hamed ,
R H Sayed ,
K Shiogama ,
M A Eltaher ,
K Suzuki  and
S Nakata
M A Hamed*
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, Sohag University, Egypt
R H Sayed
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, Sohag University, Egypt
K Shiogama
Affiliation:
Department of Pathology, Fujita Health University School of Medicine, Toyoake, Japan
M A Eltaher
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, Sohag University, Egypt
K Suzuki
Affiliation:
Department of Otorhinolaryngology, Yonaha General Hospital, Kuwana City, Japan
S Nakata
Affiliation:
Department of Otorhinolaryngology, Banbuntane Hotokukai Hospital, Fujita Health University School of Medicine, Nagoya, Japan
*
Author for correspondence: Dr Mahmood A Hamed, Department of Otorhinolaryngology, Faculty of Medicine, Sohag University, East District, Postal code 82524, Sohag, Egypt E-mail: mahmoodhamed8@gmail.com
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Abstract

Objective

To investigate the expression of basic fibroblast growth factor in the matrix of human acquired cholesteatoma compared to the deep meatal skin. This topic does not appear to have been fully investigated before.

Methods

An immunochemical study was conducted. Cholesteatoma tissues from adult patients were collected during surgery (n = 19). Control specimens were taken from the deep meatal skin (n = 8) and compared.

Results

A highly significant difference in basic fibroblast growth factor expression was identified between cholesteatoma and skin (mean ± standard error = 58.53 ± 3.6 per cent in cholesteatoma vs 40.6 ± 3.5 per cent in skin; p = 0.005). Both basal and parabasal keratinocytes were stained positive with basic fibroblast growth factor. Additionally, there was specific staining in the basal columnar middle-ear epithelium and mast cell membrane.

Conclusion

Basic fibroblast growth factor plays an active role in proliferative activity of cholesteatoma through its overexpression in basal and parabasal layers of cholesteatoma matrix. Moreover, its expression in the mast cell membrane supports its role in bone resorption activity.

Keywords

ImmunochemistryCholesteatomaMiddle EarFibroblast Growth Factor Receptor

Information

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 133 , Issue 3 , March 2019 , pp. 183 - 186
Copyright
Copyright © JLO (1984) Limited, 2019 

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Footnotes

Dr M A Hamed takes responsibility for the integrity of the content of the paper

References

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