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Insights into the genetic basis of the pre-breeding potato clones developed at the Julius Kühn Institute for high and durable late blight resistance

Published online by Cambridge University Press:  08 September 2021

Johanna Blossei Open the ORCID record for Johanna Blossei [Opens in a new window] ,
Ralf Uptmoor ,
Ramona Thieme ,
Marion Nachtigall  and
Thilo Hammann
Johanna Blossei*
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
Ralf Uptmoor
Affiliation:
Chair of Agronomy, Faculty of Agriculture and Environmental Science, University of Rostock, Rostock, Germany
Ramona Thieme
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
Marion Nachtigall
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
Thilo Hammann
Affiliation:
Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Germany
*
Author for correspondence: Johanna Blossei, E-mail: johanna.blossei@julius-kuehn.de
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Abstract

Due to the high yield losses caused by late blight in potato cultivation, the development of resistant pre-breeding material is of great importance for cultivar breeding. The gene pool of the Julius Kühn Institute (JKI) includes a large collection of resistant clones whose resistance has not yet been analysed in detail with markers for relevant resistance genes. A panel of 52 pre-breeding potato clones developed at the JKI via interspecific crosses and highly resistant to late blight were tested for the presence of seven resistance genes (Rpi-blb1/Rpi-sto1, Rpi-blb2, Rpi-blb3/R2/Rpi-abpt, R1, R3a, R3b, Rpi-phu1) and one QTL allele (QTL_phu-stn) from Solanum species S. bulbocastanum, S. demissum, S. phureja and S. stoloniferum, respectively. Molecular marker assays based on sequence-specific primers revealed that 36 of the 52 pre-breeding clones carried either 1, 2, 3 or 4 resistance genes introgressed from these wild Solanum species. Results indicate that these resistance genes were retained over generations of breeding. Although highly resistant to late blight, 16 pre-breeding clones did not carry any of these resistance genes. Resistance in the gene pool may, thus, be based not only on individual resistance genes but also on QTL effects. Results help to better understand both inheritance and expression of late blight resistance of this unique gene pool and may be used for breeding programmes.

Keywords

Crop wild relativesgene poollate blight (Phytophthora infestans)potato (Solanum tuberosum L.)resistance genes

Information

Type
Short Communication
Information
Plant Genetic Resources , Volume 19 , Issue 5 , October 2021 , pp. 461 - 464
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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References

Ballvora, A, Ercolano, MR, Weiß, J, Meksem, K, Bormann, CA, Oberhagemann, P, Salamini, F and Gebhardt, C (2002) The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. The Plant Journal 30, 361–371.CrossRefGoogle ScholarPubMed
Colton, LM, Groza, HI, Wielgus, SM and Jiang, J (2006) Marker-assisted selection for the broad-spectrum potato late blight resistance conferred by gene RB derived from a wild potato species. Crop Science 46, 589–594.CrossRefGoogle Scholar
Costanzo, S, Simko, I, Christ, BJ and Haynes, KG (2005) QTL analysis of late blight resistance in a diploid potato family of Solanum phureja × S. stenotomum. Theoretical and Applied Genetics 111, 609–617.CrossRefGoogle Scholar
Danan, S, Veyrieras, JB and Lefebvre, V (2011) Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits. BMC Plant Biology 11, 1–16.CrossRefGoogle ScholarPubMed
Dowley, LJ, Grant, J and Griffin, D (2008) Yield losses caused by late blight (Phytophthora infestans (Mont.) de Bary) in potato crops in Ireland. Irish Journal of Agriculture and Food Research 47, 69–78.Google Scholar
Ghislain, M, Byarugaba, AA, Magembe, E, Njoroge, A, Rivera, C, Román, ML, Tovar, JC, Gamboa, S, Forbes, GA, Kreuze, JF, Barekye, A and Kiggundu, A (2019) Stacking three late blight resistance genes from wild species directly into African highland potato varieties confers complete field resistance to local blight races. Plant Biotechnology Journal 17, 1119–1129.CrossRefGoogle ScholarPubMed
Haverkort, AJ, Boonekamp, PM, Hutten, R, Jacobsen, E, Lotz, LAP, Kessel, GJT, Vossen, JH and Visser, RGF (2016) Durable late blight resistance in potato through dynamic varieties obtained by cisgenesis: scientific and societal advances in the DuRPh project. Potato Research 59, 35–66.CrossRefGoogle Scholar
Huang, S, van der Vossen, EAG, Kuang, H, Vleeshouwers, VGAA, Zhang, N, Borm, TJA, van Eck, HJ, Baker, B, Jacobsen, E and Visser, RGF (2005) Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. The Plant Journal 42, 251–261.CrossRefGoogle ScholarPubMed
Kim, HJ, Lee, HR, Jo, KR, Mortazavian, SMM, Huigen, DJ, Evenhuis, B, Kessel, G, Visser, RGF, Jacobsen, E and Vossen, JH (2012) Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes. Theoretical and Applied Genetics 124, 923–935.CrossRefGoogle ScholarPubMed
Lokossou, AA, Rietman, H, Wang, M, Krenek, P, van der Schoot, H, Henken, B, Hoekstra, R, Vleeshouwers, VGAA, van der Vossen, EAG, Visser, RGF, Jacobsen, E and Vosman, B. (2010) Diversity, distribution, and evolution of Solanum bulbocastanum late blight resistance genes. Molecular Plant-Microbe Interactions Journal 23, 1206–1216.CrossRefGoogle ScholarPubMed
OEPP/EPPO (2021) PP 1/002(5) Phytophthora infestans on potato. Bulletin OEPP/EPPO Bulletin 51, 79–82.10.1111/epp.12708CrossRefGoogle Scholar
Rakosy-Tican, E, Thieme, R, König, J, Nachtigall, M, Hammann, T, Denes, TE, Kruppa, K and Molnár-Láng, M (2020) Introgression of two broad-spectrum late blight resistance genes, Rpi-Blb1 and Rpi-Blb3, from Solanum bulbocastanum dun plus race-specific R genes into potato pre-breeding lines. Frontiers in Plant Science 11, 699.CrossRefGoogle ScholarPubMed
Rietman, H (2011) Putting the Phytophthora Infestans Genome Sequence at Work; Identification of Many New R and Avr Genes in Solanum (PhD thesis), Wageningen University, Wageningen, The Netherlands.Google Scholar
Rogozina, EV, Beketova, MP, Muratova, OA, Kuznetsova, MA and Khavkin, EE (2021) Stacking resistance genes in multiparental interspecific potato hybrids to anticipate late blight outbreaks. Agronomy 11, 1–31.CrossRefGoogle Scholar
Śliwka, J, Jakuczun, H, Lebecka, R, Marczewski, W, Gebhardt, C and Zimnoch-Guzowska, E (2006) The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period. Theoretical and Applied Genetics 113, 685–695.CrossRefGoogle Scholar
Stefańczyk, E, Plich, J, Janiszewska, M, Smyda-Dajmund, P, Sobkowiak, S and Śliwka, J (2020) Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di-and tetraploid levels. Molecular Breeding 40, 1–12.CrossRefGoogle Scholar
Stewart, HE, Bradshaw, JE and Pande, B (2003) The effect of the presence of R-genes for resistance to late blight (Phytophthora infestans) of potato (Solanum tuberosum) on the underlying level of field resistance. Plant Pathology 52, 193–198.CrossRefGoogle Scholar
Van der Vossen, E, Sikkema, A, Hekkert, BtL, Gros, J, Stevens, P, Muskens, M, Wouters, D, Pereira, A, Stiekema, W and Allefs, S (2003) An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. The Plant Journal 36, 867–882.CrossRefGoogle ScholarPubMed
Vleeshouwers, VGAA, Raffaele, S, Vossen, JH, Champouret, N, Oliva, R, Segretin, ME, Rietmann, H, Cano, LM, Lokossou, A, Kessel, G, Pel, MA and Kamoun, S (2011) Understanding and exploiting late blight resistance in the age of effectors. Annual Review of Phytopathology 49, 507–531.CrossRefGoogle ScholarPubMed
Wang, M, Allefs, S, van den Berg, RG, Vleeshouwers, VGAA, van der Vossen, EAG and Vosman, B (2008) Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum. Theoretical and Applied Genetics 116, 933–943.CrossRefGoogle ScholarPubMed
Wickramasinghe, WMDK, Qu, XS, Costanzo, S, Haynes, KG and Christ, BJ (2009) Development of PCR-based markers linked to quantitative resistance to late blight in a diploid hybrid potato population of Solanum phureja × S. stenotomum. American Journal of Potato Research 86, 188–195.10.1007/s12230-009-9071-5CrossRefGoogle Scholar
Wiik, L (2014) Potato late blight and tuber yield: results from 30 years of field trials. Potato Research 57, 77–98.10.1007/s11540-014-9256-2CrossRefGoogle Scholar
Zhu, S, Li, Y, Vossen, JH, Visser, RGF and Jacobsen, E (2012) Functional stacking of three resistance genes against Phytophthora infestans in potato. Transgenic Research 21, 89–99.CrossRefGoogle ScholarPubMed
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