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RETRACTED – Ultrastructural study: in vitro and in vivo differentiation of mice spermatogonial stem

Published online by Cambridge University Press:  27 December 2023

Zahra Bashiri ,
Mansoureh Movahedin ,
Vahid Pirhajati ,
Hamidreza Asgari  and
Morteza Koruji Open the ORCID record for Morteza Koruji [Opens in a new window]
Zahra Bashiri
Affiliation:
Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran Omid Fertility & Infertility Clinic, Hamedan, Iran
Mansoureh Movahedin
Affiliation:
Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Vahid Pirhajati
Affiliation:
Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran
Hamidreza Asgari
Affiliation:
Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Morteza Koruji*
Affiliation:
Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
*
Corresponding author: Morteza Koruji; Email: koruji.m@iums.ac.ir
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Summary

Mouse testicular tissue is composed of seminiferous tubules and interstitial tissue. Mammalian spermatogenesis is divided into three stages: spermatocytogenesis (mitotic divisions) in which spermatogonial stem cells (SSCs) turn into spermatocytes, followed by two consecutive meiotic divisions in which spermatocytes form spermatids. Spermatids differentiate into spermatozoa during spermiogenesis. Various factors affect the process of spermatogenesis and the organization of cells in the testis. Any disorder in different stages of spermatogenesis will have negative effects on male fertility. The aim of the current study was to compare the in vitro and in vivo spermatogenesis processes before and after transplantation to azoospermic mice using ultrastructural techniques. In this study, mice were irradiated with single doses of 14 Gy 60Co radiation. SSCs isolated from neonatal mice were cultured in vitro for 1 week and were injected into the seminiferous tubule recipient’s mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells in vitro (after 3 weeks) were then processed for transmission electron microscopy studies. Our study’s findings revealed that the morphology and ultrastructure of testicular cells after transplantation and in vitro culture are similar to those of in vivo spermatogenesis, indicating that spermatogenic cell nature is unaltered in vitro.

Keywords

In vitro cultureIrradiationSpermatogonia stem cellsTransplantationUltrastructure

Information

Type
Research Article
Information
Zygote , Volume 32 , Issue 1 , February 2024 , pp. 87 - 95
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© The Author(s), 2023. Published by Cambridge University Press

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