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Molecular subtyping by genome and plasmid analysis of Campylobacter jejuni serogroups O1 and O2 (Penner) from sporadic and outbreak cases of human diarrhoea

Published online by Cambridge University Press:  15 May 2009

A. Fayos ,
R. J. Owen ,
J. Hernandez ,
C. Jones  and
A. Lastovica
A. Fayos
Affiliation:
Departamento Biotecnologia, Universidad Politecnica, Valencia, Spain
R. J. Owen*
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, Colindale Avenue, London NW9 5HT, UK
J. Hernandez
Affiliation:
Departamento Biotecnologia, Universidad Politecnica, Valencia, Spain
C. Jones
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, Colindale Avenue, London NW9 5HT, UK
A. Lastovica
Affiliation:
Department of Microbiology, Red Cross War Memorial Children's Hospital, Cape Town, Republic of South Africa
*
*Corresponding author
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Ribosomal RNA gene patterns, randomly amplified polymorphic genomic DNA (RAPD) profiles and plasmid profiles were used to discriminate between 28 strains of Campylobacter jejuni serogroups O1 and O2 (Penner). Most isolates were biotype I (Lior). The strains were representative isolates from a UK school outbreak of enteritis (7 cases) and from 21 sporadic human cases of enteritis in 4 countries. The molecular techniques discriminated to various degrees between strains in each of the serogroups. The outbreak strains were homogeneous in most molecular features but a variety of types was detected amongst the isolates from the sporadic cases. Five groups of two or more strains with identical ribopatterns were identified and within each, strains from different patients were homogenous with respect to serogroup. RAPD profile typing based on numerical analysis generally matched ribotyping. Plasmid profiling overall gave least discrimination but was useful in separating some strains similar in other features. We concluded that optimal discrimination of C. jejuni could best be achieved using a combination of phenotypic and genotypic properties. Hae III ribotyping was the single most discriminatory and reproducible technique investigated. Several strains of C. jejuni from sporadic infections had similar molecular profiles which have potential for general typing purposes.

Information

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 3 , December 1993 , pp. 415 - 428
Copyright
Copyright © Cambridge University Press 1993

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