The adequate intake of α-linolenic acid (cis-9, cis-12, cis-15 18:3) has been established at 1.6 g/d for adult men and 1.1 g/d for adult women (Flock et al., Reference Flock, Harris and Kris-Etherton2013). Dairy products may contribute to a low proportion of this recommendation, as the consumption of two servings of regular whole milk (3.25% fat) containing 4.1 mg cis-9, cis-12, cis-15 18:3/g of fatty acids (FA; Heck et al., Reference Heck, Van Valenberg, Bovenhuis, Dijkstra and Van Hooijdonk2012) brings only 33 mg of this essential FA.
Linseed (also called flaxseed; Linum usitatissimum) is a rich source of cis-9, cis-12, cis-15 18:3 (INRA-AFZ, Reference Sauvant, Perez and Tran2004). This oilseed has been evaluated for its potential to increase the concentration of n-3 FA in milk fat. In this regard, a meta-analysis by Leduc et al. (Reference Leduc, Létourneau-Montminy, Gervais and Chouinard2017) has shown that the transfer efficiency of dietary cis-9, cis-12, cis-15 18:3 varied from 1.95%, with diets supplemented with linseed oil (L-oil), to 5.84% with diets based on mechanically treated whole linseed. As a result of this transfer, consumption of food products (including milk) from animals fed linseed has been associated with positive effects on blood lipid profile in humans (Weill et al., Reference Weill, Schmitt, Chesneau, Daniel, Safraou and Legrand2002).
Unfortunately, experiments have shown that milk enriched in cis-9, cis-12, cis-15 18:3 is highly prone to oxidative deterioration (Fauteux et al., Reference Fauteux, Gervais, Rico, Lebeuf and Chouinard2016; Rico et al., Reference Rico, Gervais, Schwebel, Lebeuf and Chouinard2021). Oxidation of polyunsaturated FA has been associated with the development of undesirable flavours (eg rancidity) and potentially toxic chemicals (Arab-Tehrany et al., Reference Arab-Tehrany, Jacquot, Gaiani, Imran, Desobry and Linder2012), which can impair the nutritional and sensory properties of dairy products. Liu et al. (Reference Liu, Wang, Bu, Liu, Wei, Zhou and Beitz2010) studied the effects of graded amounts of cis-9, cis-12, cis-15 18:3 infused into the duodenum, from 0 to 132 g/d, resulting in a linear increase of milk fat concentration of this FA from 0.6 to 25.4%. This modification of milk FA profile quadratically decreased the activity of enzymatic radical scavenging systems, such as superoxide dismutase, glutathione peroxidase and catalase (Liu et al., Reference Liu, Wang, Bu, Liu, Wei, Zhou and Beitz2010). However, the subsequent consequences on the development of volatile degradation products such as aldehydes and ketones responsible for the development of milk off-odours and off-flavours have not been assessed.
Our objective was to study the effect of increasing postruminal supply of L-oil as a source of cis-9, cis-12, cis-15 18:3 on milk FA profile, and to assess the resulting impact on the development of volatile degradation products during the storage of homogenized milk.
Materials and methods
Animals and treatments
The experimental procedures involving dairy cows followed the guidelines of the Canadian Council on Animal Care (2009) and were approved by the Université Laval Animal Care Committee (Protocol # 2015001). Information about cows, feeding, treatments, and experimental design is reported in a companion paper (Gervais et al., Reference Gervais, Rico, Peňa-Cotrino, Lebeuf and Chouinard2023, In press). Briefly, 5 Holstein dairy cows (36 ± 2 d postpartum; mean ± sd) were randomly distributed in a 5 × 5 Latin square design with periods of 21 d. All cows were fed the same total mixed ration. During the first 14 d of each period, L-oil (Pokonobe Industries Inc., Westmount, QC; containing 5.6% 16:0, 3.4% 18:0, 18.4% cis-9 18:1, 0.7% cis-11 18:1, 14.9% cis-9, cis-12 18:2, 55.9% cis-9, cis-12, cis-15 18:3, and 0.2% 20:0) was abomasally infused at 0, 75, 150, 300, and 600 mL/d using peristaltic pumps. Infusions were followed by a 7-d washout interval.
Sampling, measurements, and analyses
Dry matter intake and milk yield were recorded, and samples of feed and milk were harvested during the last 3 d of each infusion period. Data on dry matter intake, milk production, as well as concentration and yield of major milk constituents were reported previously (Gervais et al., Reference Gervais, Rico, Peňa-Cotrino, Lebeuf and Chouinard2023, In press). An additional set of milk samples without preservative was harvested during the last 3 d of each infusion and stored at −20°C for later determination of the FA profile following the procedure described by Boivin et al. (Reference Boivin, Gervais and Chouinard2013), and reported in the online Supplementary File, material and methods. Glycerol in milk fat was calculated as described by Stamey et al. (Reference Stamey, Corl, Chouinard and Drackley2010).
A last set of milk samples for oxidative stability analyses was collected during the morning milking on day 11 of each infusion period. These samples were immediately transported to Université Laval pilot plant in 1-L stainless-steel cans, while being kept on ice until processed as described by Fauteux et al. (Reference Fauteux, Gervais, Rico, Lebeuf and Chouinard2016). Briefly, milk samples were heated at 50°C, homogenized at 24 MPa (EmulsiFlex-C50, Avestin, Ottawa, ON, Canada), and then cooled at 4°C.
Oxidation was induced by the addition of 0.001% Fe (as FeSO4). Samples were stored horizontally for 0, 2, 4, 7, and 11 d in two sets of glass tubes at 4°C in a cabinet under fluorescent light (warm white, linear T12, 40 W; Lumisolution Inc., Québec, QC, Canada). Sodium azide (0.02%) was added to prevent microbial growth. Samples in the first set of tubes were analysed for 1-octen-3-one, propanal, hexanal, trans-2 + cis-3-hexenals, cis-4-heptenal, trans-2, cis-6-nonadienal, and trans-2, trans-4-nonadienal. The second set of tubes was analysed for redox potential, as well as conjugated diene and triene hydroperoxides. The same analyses were performed on fresh non-homogenized milk on day 0. A second subsample of fresh milk was stored at −20°C, without preservative, until analysed for FA profile as described above. In order to assess susceptibility of milk fat to oxidation as affected by unsaturated FA content, a peroxidability index (PI) was calculated for each sample based on milk concentrations of monoenoic (Mono), dienoic (Di), trienoic (Tri), tetraenoic (Tetra), pentaenoic (Penta), and hexaenoic (Hexa) FA (Witting and Horwitt, Reference Witting and Horwitt1964), as follows:
$${\rm PI}\,{\rm} = ( { 0.025\,\times \,{\rm Mono}} ) \,{\rm} + ( { 1\,\times \,{\rm Di}} ) \,{\rm} + ( { 2\,\times \,{\rm Tri}} ) \,{\rm} + ( { 4\,\times \,{\rm Tetra}} ) \,{\rm} + ( { 6\,\times \,{\rm Penta}} ) \,{\rm} + ( { 8\,\times \,{\rm Hexa}} ) $$to account for individual oxidation sensitivity of FA. The use of concentrations of these FA groups as a proportion of milk constituents, rather than as a proportion of total fat, was intended to account for the variation in substrate availability for peroxidation resulting from differences in milk fat concentration among samples.
Analyses of secondary lipid oxidation products of fresh and stored milk were conducted using the solid-phase microextraction technique with a Combi PAL autosampler (CTC Analytics, Zwingen, Switzerland) attached to an Agilent 6890N gas chromatograph with a 5973 inert mass spectrometry detection (Agilent Technologies Canada Inc.) as previously described by Fauteux et al. (Reference Fauteux, Gervais, Rico, Lebeuf and Chouinard2016). Finally, redox potential and conjugated diene and triene hydroperoxides were analysed as reported in the online Supplementary File, Material and methods.
Statistical analysis
Data were analysed using the MIXED procedure of SAS 9.4 (SAS Institute Inc, Cary, NC, USA). For variables where repeated measures were not performed, the following model was fit:
$$Y_{{\rm ijkl}}\,{\rm} = {\rm \mu }\,{\rm} + T_{\rm i}\,{\rm} + P_{\rm j}\,{\rm} + S_{\rm k}\,{\rm} + C_{\rm l}\,( S_{\rm k}) \,{\rm} + {\rm \varepsilon }_{{\rm ijkl}}$$where Y ijkl is the individual observation, μ the overall mean, T i the fixed effect of treatment (i = 1–5), P j the random effect of period (j = 1–5), S k the random effect of sequence (k = 1–5), C l(S k) the random effect of cow (l = 1–5) nested in sequence, and ɛijkl the residual error terms. Linear and quadratic contrasts for treatment effect were performed.
For variables submitted to repeated measures, data were analysed according to the following model:
$$Y_{{\rm ijklm}}\,{\rm} = {\rm \mu }\,{\rm} + T_{\rm i}\,{\rm} + P_{\rm j}\,{\rm} + S_{\rm k}\,{\rm} + C_{\rm l}\,( S_{\rm k}) \,{\rm} + D_{\rm m}\,{\rm} + {\rm T}{\rm D}_{{\rm im}}\,{\rm} + {\rm \varepsilon }_{{\rm ijklm}}$$where Y ijklm is the individual observation, μ the overall mean, T i the fixed effect of treatment (i = 1–5), P j the random effect of period (j = 1–5), S k the random effect of sequence (k = 1–5), C l(S k) the random effect of cow (l = 1–5) nested in sequence, D m the effect of day (m = 1–5) + TDim the interaction of treatment and day, and ɛijklm the residual error terms. Linear and quadratic contrasts for the effects of treatment and day were performed. Differences between treatments were declared at P ≤ 0.05.
Results
Complete data are provided in the online Supplementary File for milk fat composition (Table S1), oxidative stability parameters (Table S2) and changes in redox potential and volatile products during storage (Table S3). The concentration of cis-9, cis-12 18:2 and cis-9, cis-12, cis-15 18:3 in milk fat increased linearly with L-oil dose (Fig. 1). Conversely, the concentration of 14:0 decreased linearly, and the concentration 16:0 decreased linearly and quadratically with the level of L-oil. Proportions of 6:0, 8:0, 10:0, 12:0, 18:0, and cis-9 18:1 were not affected by L-oil infusion (Fig. 1). The PI increased linearly with the level of L-oil (Fig. 2).
Milk fat concentrations of 6:0 (a), 8:0 (b), 10:0 (c), 12:0 (d), 14:0 (e), 16:0 (f), 18:0 (g), cis-9 18:1 (h), cis-9, cis-12 18:2 (i), and cis-9, cis-12, cis-15 18:3 (j) in dairy cows abomasally infused with increasing levels of linseed oil. sem = standard error of the mean. L, linear, and Q, quadratic effect of the level of linseed oil infusion. *P ≤ 0.05 and **P ≤ 0.01. NS, not significantly affected (P > 0.10). See online Supplementary File, Table S1 for complete fatty acid profiles.
Peroxidability index (a), redox potential (b), and concentrations of propanal (c), hexanal (d), trans-2 + cis-3-hexenals (e), cis-4-heptenal (f), and conjugated diene (g) and triene (h) hydroperoxides in fresh milk of dairy cows abomasally infused with increasing levels of linseed oil. sem, standard error of the mean. L, linear and Q, quadratic effect of the level of linseed oil infusion. *P ≤ 0.05 and **P ≤ 0.01. NS, not significantly affected (P > 0.05). Table values can be found in online Supplementary File, Table S2.
In fresh milk, redox potential as well as concentrations of propanal, hexanal, trans-2 cis-3-hexenals, cis-4-heptenal, and conjugated diene hydroperoxide increased linearly with the dose of L-oil (Fig. 2). The concentration of conjugated triene hydroperoxides was not affected, whereas trans-2, cis-6-nonadienal and trans-2, trans-4-nonadienal were not detected in fresh milk.
Redox potential and concentrations of all nine lipid oxidation products evaluated increased during the storage of homogenized milk under light exposure (Fig. 3). The magnitude of the difference between final and initial measurements increased linearly with the level of infusion for the concentrations of 1-octen-3-one, propanal, hexanal, trans-2 + cis-3-hexenals, cis-4-heptenal, trans-2, cis-6-nonadienal trans-2, trans-4-nonadienal, and conjugated diene and triene hydroperoxides (Fig. 4).
Effect of time of storage under light exposure on redox potential (a) and on concentrations of 1-octen-3-one, (b) propanal (c), hexanal (d), trans-2 + cis-3-hexenals, (e) cis-4-heptenal (f), trans-2, cis-6-nonadienal (g), trans-2, trans-4-nonadienal (h), and conjugated diene (i) and triene (j) hydroperoxides in homogenized milk from cows abomasally infused with linseed oil at the rate of 0 (×), 75 (⬤), 150 (■), 300 (▲), and 600 (◆) ml/d. TL, Linear effect of treatment; TQ, Quadratic effect of treatment; LL, Linear effect of infusion level; LQ, Quadratic effect of infusion level, *P ≤ 0.05 and **P ≤ 0.01. NS, not significantly affected (P > 0.05).
Variations of redox potential (a) and in concentrations of 1-octen-3-one, (b) propanal (c), hexanal (d), trans-2 + cis-3-hexenals, (e) cis-4-heptenal (f), trans-2, cis-6-nonadienal (g), trans-2, trans-4-nonadienal (h), and conjugated diene (i) and triene (j) hydroperoxides during storage of homogenized milk from dairy cows abomasally infused with increasing levels of linseed oil. Data represent the difference between final (day 11) and initial (day 0) redox potential and concentrations of each component following storage at 4°C under fluorescent light. sem, standard error of the mean; L, linear and Q, quadratic effect of the level of linseed oil infusion. *P ≤ 0.05 and **P ≤ 0.01. NS, not significantly affected (P > 0.05). Table values can be found in online Supplementary File, Table S3.
Discussion
Here, abomasal infusion of L-oil has been effective in increasing milk fat concentration of polyunsaturated FA. In particular, the proportions of cis-9, cis-12 18:2 and cis-9, cis-12, cis-15 18:3 increased by 2.3- and 16.4-fold, respectively, when infusing 600 ml/d of L-oil as compared with control (no infusion). The greatest level of cis-9, cis-12, cis-15 18:3 (9.0 g/100 g fat) is equivalent to 772 mg in a glass of whole milk (3.25% fat). At this concentration, adequate intake of cis-9, cis-12, cis-15 18:3 for adult women (1.1 g/d) and men (1.6 g/d; Flock et al., Reference Flock, Harris and Kris-Etherton2013) could be achieved by the consumption of 2 servings of milk per day. These increases were mainly compensated by decreased concentrations of 14:0 and 16:0. Similar effects on milk FA profile were reported by Lima et al. (Reference Lima, Palin, Santos, Benchaar, Lima, Chouinard and Petit2014) in cows abomasally infused with L-oil.
Fatty acids are not distributed randomly in milk TAG (Jensen, Reference Jensen2002). By feeding formaldehyde treated sunflower seeds as a source of ruminally protected unsaturated FA, Morrison and Hawke (Reference Morrison and Hawke1977a) observed an increase in the concentration of cis-9, cis-12 18:2 from 1.8 to 15.5% of milk FA, on a molar basis, mostly at the expense of 14:0 and 16:0. Bovine milk fat can be divided into high- and low-molecular-weight TAG. Morrison and Hawke (Reference Morrison and Hawke1977b) reported the stereospecific distribution of FA in the high molecular weight fractions and showed that increases in cis-9, cis-12 18:2 in each of the three positions of TAG were paralleled by decreases in the levels of 14:0 and 16:0. More specifically, the preference of cis-9, cis-12 18:2 for position sn-3 over position sn-1 was shown to divert the available 14:0 and 16:0 into position sn-1 at the expense of position sn-3. In our research, a similar phenomenon could explain lower proportions of 14:0 and 16:0 in milk fat in response to increasing levels of cis-9, cis-12 18:2 and cis-9, cis-12, cis-15 18:3 from abomasally infused L-oil. Such disruption of TAG synthesis, due to increase availability of polyunsaturated FA for esterification, could also potentially explain the lower milk fat concentration and yield observed in response to increasing levels of infusion (Gervais et al., Reference Gervais, Rico, Peňa-Cotrino, Lebeuf and Chouinard2023, In press). The substitution of polyunsaturated for saturated FA linearly increased the PI of milk fat, from 2.0 mg/g milk in the control to 10.8 mg/g milk at the highest dose. These milk samples, with increasing PI, were submitted to oxidative conditions with the addition of FeSO4 and storage at 4°C under fluorescent light. Conjugated diene and triene hydroperoxides were determined as primary lipid oxidation products. Both cis-9, cis-12 18:2 and cis-9, cis-12, cis-15 18:3 are known to form conjugated diene hydroperoxides. Conjugated trienes are produced from cis-9, cis-12, cis-15 18:3 when two positions of the carbon chain are attacked (Patterson, Reference Patterson1989). Alternatively, conjugated trienes may be produced by dehydration of conjugated diene hydroperoxides (Fishwick and Swoboda, Reference Fishwick and Swoboda1977).
We observed that the concentration of conjugated dienes in fresh milk was about 4.8 times greater compared with conjugated trienes. These concentrations increased quadratically over time, reaching a plateau at 7 d of storage in milk from cows receiving the highest level of L-oil infusion (600 ml/d). Hydroperoxides eventually break down to secondary lipid oxidation products (Patterson, Reference Patterson1989). In this regard, hexanal (Frankel, Reference Frankel1982) and 1-octen-3-one (Ullrich and Grosch, Reference Ullrich and Grosch1987) are volatile products expected from cis-9, cis-12 18:2 oxidation. Propanal, cis-4 heptenal, cis-3-hexenal, trans-2-hexenal, trans-2, cis-6-nonadienal, and trans-2, trans-4-nonadienal arise from oxidation of cis-9, cis-12, cis-15 18:3 (Frankel, Reference Frankel1982; Josephson and Lindsay, Reference Josephson and Lindsay1987; Ullrich and Grosch, Reference Ullrich and Grosch1988). Concentrations of secondary lipid oxidation products increased exponentially during storage in our experiments. This observation is consistent with the fact that oxidative progression is autocatalytic and needs only one initiating radical to begin the production of hydroperoxides (Timmons et al., Reference Timmons, Weiss, Palmquist and Harper2001). Such phenomena may explain why the concentrations of secondary lipid oxidation products intensified as storage time increases. After 11 d under fluorescent light, the overall increase (d 11 minus d 0) in the concentrations of these secondary products was enhanced linearly with the level of L-oil infusion.
In conclusion, our results have shown that milk enriched in cis-9, cis-12 18:2 and cis-9, cis-12, cis-15 18:3 via postruminal supply of L-oil is highly prone to oxidative degradation. Attempts have been made in the past to prevent oxidation of milk containing high levels of polyunsaturated FA (Fauteux et al., Reference Fauteux, Gervais, Rico, Lebeuf and Chouinard2016; Rico et al., Reference Rico, Gervais, Schwebel, Lebeuf and Chouinard2021) using dietary treatments aimed to increase levels of vitamin E, carotenoids or enterolactones. None of these interventions have been efficient in significantly reducing the production of primary and secondary volatile oxidation products known for their impacts on organoleptic properties of milk (Bendall, Reference Bendall2001). This low oxidative stability, exposed under controlled experimental conditions, would represent a major obstacle to commercial initiatives to market milk enriched in polyunsaturated FA.
Supplementary material
The supplementary material for this article can be found at https://doi.org/10.1017/S0022029923000262
Acknowledgements
This experiment was funded through Industrial Research Chair program of the Natural Sciences and Engineering Research Council of Canada (Ottawa, ON, Canada), with industry contributions from the Dairy Farmers of Canada (Ottawa, ON, Canada), Novalait Inc. (Québec, QC, Canada), Valacta (Sainte-Anne-de-Bellevue, QC, Canada), Les Producteurs de Lait du Québec (Longueuil, QC, Canada), and the Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec (Québec, QC, Canada). The authors thank administrative and research staff of the Centre de Recherche en Sciences Animales de Deschambault (Deschambault, QC, Canada) for the care provided to cows during the trial.
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