Hostname: page-component-6766d58669-kl59c Total loading time: 0 Render date: 2026-05-20T05:54:07.702Z Has data issue: false hasContentIssue false
  • English
  • Français

Pars flaccida perforation in tissue culture: morphological study

Published online by Cambridge University Press:  29 June 2007

L.-E. Stenfors ,
E. G. Olsen  and
A. Ö. Henriksen
L.-E. Stenfors*
Affiliation:
Department of Otolaryngology, University of Tromsö, Tromsö, Norway.
E. G. Olsen
Affiliation:
Department of Ophthalmology, University of Tromsö, Tromsö, Norway.
A. Ö. Henriksen
Affiliation:
Department of Otolaryngology, University of Tromsö, Tromsö, Norway.
*
Dr Lars-Eric Stenfors, Idrottsgatan 34, 67200 Karleby, Finland.
Get access Rights & Permissions [Opens in a new window]

Abstract

Subtotal pars flaccida perforation was effected in vitro in rat tympanic membranes. The drums were subsequently kept in tissue culture and after various intervals studied by means of light and scanning electron microscopy. No complete covering of the perforation was seen, though a marked thickening and hyperplasia of the outer, keratinizing, squamous epithelium (OE) was evident. The inner, tympanal epithelium (IE) appeared swollen, containing rounded structures in the cytoplasm especially close to the contact area with the OE. Ingrowth of OE onto the tympanal side of the drum was seen particularly in the areas where the IE was sparse and completely lacking. Complete covering of a drum perforation seems to be dependent on the formation of supporting granulation tissue, probably resulting from an inflammatory reaction in the healing area.

Information

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 104 , Issue 2 , February 1990 , pp. 91 - 96
Copyright
Copyright © JLO (1984) Limited 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Boedts, D. (1978) The tympanal epithelium in normal and pathological conditions. Acta Oto-rhino-laryngologica (Belgica) 32: 295–420.Google ScholarPubMed
Clawson, J. P., Litton, W. B. (1971) The healing process of tympanic membrane perforations. Transactions of the American Academy of Ophthalmology and Otolaryngology 75: 1302–1312.Google Scholar
Davanger, M., Olsen, E. G. (1985) Experimental epithelial ingrowth. Epithelial/endothelial interaction through a corneal perforation, studied in organ culture. Acta Ophthalmologica 63: 443–448.Google Scholar
Medin, W., Davanger, M. (1987) Wound healing of rabbit cornea in organ culture. Acta Ophthalmologica 65: 257–262.Google Scholar
McMinn, R. M. H., Taylor, M. (1966) The cytology of repair in experimental perforations of the tympanic membrane. British Journal of Surgery 53: 222–232.CrossRefGoogle ScholarPubMed
Reeve, D. R. E. (1977) Repair of large experimental perforations of the tympanic membrane. Journal of Laryngology and Otology 91: 767–778.Google Scholar
Reynen, C. J. H., Kuypers, W. (1971) The healing pattern of drum membrane. Acta Otolaryngologica Supplement. 287.Google Scholar
Schuknecht, H. F. (1974) Pathology of the Ear, p. 216. Harvard University Press, Cambridge, Massachusetts.Google Scholar
Stenfors, L.-E., Carlsöö, B., Salzén, B., Winblad, B. (1980 a) Repair of experimental tympanic membrane perforations. Acta Otolaryngologica 90: 332–341.CrossRefGoogle ScholarPubMed
Stenfors, L.-E., Salén, B. and Winblad, B. (1980 b) The healing pattern of experimental pars flaccida perforations. Acta Otolaryngologica 90: 261–274.Google Scholar