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Developing polyploid genetic resources for enhanced rebaudioside A synthesis and agronomic traits in Stevia rebaudiana Bertoni

Published online by Cambridge University Press:  27 December 2024

Sivendra Joshi Open the ORCID record for Sivendra Joshi [Opens in a new window] ,
Venkatesha K. T. Open the ORCID record for Venkatesha K. T. [Opens in a new window] ,
Abhilasha Gupta ,
Rajendra Chandra Padalia  and
Dipender Kumar
Sivendra Joshi
Affiliation:
CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Research Centre, Pantnagar, Udham Singh Nagar, Uttarakhand-263149, India
Venkatesha K. T.*
Affiliation:
CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Research Centre, Pantnagar, Udham Singh Nagar, Uttarakhand-263149, India
Abhilasha Gupta
Affiliation:
CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Research Centre, Pantnagar, Udham Singh Nagar, Uttarakhand-263149, India
Rajendra Chandra Padalia
Affiliation:
CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Research Centre, Pantnagar, Udham Singh Nagar, Uttarakhand-263149, India
Dipender Kumar
Affiliation:
CSIR-Central Institute of Medicinal and Aromatic Plants (CIMAP), Research Centre, Pantnagar, Udham Singh Nagar, Uttarakhand-263149, India
*
Corresponding author: Venkatesha K. T.; Email: venkatesha@cimap.res.in
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Abstract

Stevia rebaudiana Bertoni stores over 20 glycosides in leaves and stem, the primary sweetening compounds are stevioside and rebaudioside A. Stevioside is 250–300 times and rebaudioside A is 350–450 times sweeter than sucrose. These glycosides can be substituted for free sugars being used in food and beverage industries. Due to limitations in the improvement of stevia through conventional breeding, induced polyploidy technique is a quick way to achieve enhanced leaf yield with increased secondary metabolites production. Presented study was conducted to develop genetic resources by inducing polyploidy and characterizing it. The apical meristem of 30-day-old seedlings was treated with colchicine solutions, ranging from 0.05 to 0.30%. Flow-cytometric analysis confirmed 13 tetraploids and one triploid genotype. Tetraploid plants showed significant changes in plant height, had larger leaf area, thicker leaves and higher levels of stevioside and rebaudioside A content, as compared to control. The triploid plant also surpassed the diploid control in number of branches per plant, leaf thickness, leaf area and fresh stem weight. The tetraploid line (ST 6) had 65.7% higher rebaudioside A content compared to diploid control. Correlation study revealed that leaf area and leaf thickness had an inverse correlation with rebaudioside A content. These newly developed polyploid genotypes offer valuable genetic resources for future stevia breeding programmes and as polyploid varieties for commercial cultivation.

Keywords

morphochemical traitspolyploidy breedingrebaudioside Asteviosidetetraploidstriploid

Information

Type
Research Article
Information
Plant Genetic Resources , Volume 23 , Issue 3 , June 2025 , pp. 156 - 164
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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