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Plasmablastic lymphoma of the larynx: report of two cases

Published online by Cambridge University Press:  19 September 2012

K Stephenson ,
S Peer ,
D Govender  and
J J Fagan
K Stephenson*
Affiliation:
Division of Otorhinolaryngology, University of Cape Town, South Africa
S Peer
Affiliation:
Division of Otorhinolaryngology, University of Cape Town, South Africa
D Govender
Affiliation:
Division of Anatomical Pathology, University of Cape Town, South Africa
J J Fagan
Affiliation:
Division of Otorhinolaryngology, University of Cape Town, South Africa
*
Address for correspondence: Dr Kate Stephenson, Division of Otorhinolaryngology, University of Cape Town, H-53 Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa E-mail: katestephenson@doctors.org.uk
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Abstract

Objective:

To report two cases of laryngeal plasmablastic lymphoma, a rare and relatively recently described form of non-Hodgkin's lymphoma. It has not previously been described in the larynx, nor associated with upper airway obstruction.

Case reports:

We describe the clinicopathological features of two such cases in human immunodeficiency virus positive patients, and we discuss their unusual presentations and diagnostic features.

Conclusion:

When evaluating a laryngeal tumour, plasmablastic lymphoma and other non-Hodgkin's lymphomata should be considered as differential diagnoses, particularly in the setting of a high prevalence of human immunodeficiency virus infection. Biopsy with detailed histopathological and immunohistochemical evaluation is recommended to ensure correct diagnosis and optimal management.

Keywords

LarynxPlasmablastic LymphomaLymphoma, Non-Hodgkin'sLymphoma, B-CellHuman Immunodeficiency Virus

Information

Type
Clinical Records
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 1 , January 2013 , pp. 96 - 99
Copyright
Copyright © JLO (1984) Limited 2012

Introduction

Plasmablastic lymphoma is a rare and relatively recently described form of non-Hodgkin's lymphoma. It has not previously been described in the larynx, nor associated with upper airway obstruction. In this paper, we describe the clinicopathological features of two cases of plasmablastic lymphoma occurring in the larynx, and we highlight the unusual presentation and diagnostic features.

In 1997, Delecluse et al. were the first to describe a new, distinct subtype of B-cell lymphoma of the oral cavity, in a series of 16 patients.Reference Delecluse, Anagnostopoulos, Dallenbach, Hummel, Marafioti and Schneider 1 Following this initial report of oral tumours, a number of extra-oral sites have been described; these include the paranasal sinuses, skin and soft tissue, lung, and anorectal region.Reference Castillo and Reagan 2 Plasmablastic lymphoma has an aggressive nature and a particularly poor prognosis.Reference Castillo, Pantanowitz and Dezube 3

Plasmablastic lymphoma may present a significant diagnostic challenge. Distinct morphological and clinical features have been recognised, and a clear association between immunosuppression and plasmablastic lymphoma has been identified. In a recent review of 228 cases of plasmablastic lymphoma, approximately 70 per cent of cases were described in individuals infected with human immunodeficiency virus (HIV).Reference Castillo, Winer, Stachurski, Perez, Jabbour and Milani 4 Plasmablastic lymphoma accounts for an estimated 2.6 per cent of all HIV-associated non-Hodgkin's lymphoma.Reference Carbone 5 An estimated further third of HIV-negative plasmablastic lymphoma patients have been found to be otherwise immunodeficient, for example, as a result of steroid therapy or solid organ transplantation.Reference Rafaniello Raviele, Pruneri and Maiorano 6 Non-Hodgkin's lymphoma is classified as an acquired immune deficiency syndrome (AIDS) defining condition; furthermore, the risk of non-Hodgkin's lymphoma in HIV-infected persons is increased in those with more advanced immunodeficiency.Reference Bohlius, Schmidlin, Costagliola, Fätkenheuer, May and Caro-Murillo 7 Meta-analysis of cohort studies has also confirmed that the incidence of HIV-related non-Hodgkin's lymphoma has decreased since the advent of highly active anti-retroviral therapy (HAART).Reference Thirlwell, Sarker, Stebbing and Bower 8

The pathogenesis of plasmablastic lymphoma is poorly understood. In common with other HIV-related non-Hodgkin's lymphomas, Epstein–Barr virus is suspected to be involved, and potential mechanisms have recently been suggested.Reference Carbone, Cesarman, Spina, Gloghini and Schulz 9 A recent meta-analysis found that 74 per cent of HIV-associated plasmablastic lymphoma patients showed the presence of Epstein–Barr virus, detected by in situ hybridisation and/or polymerase chain reaction in more than 90 per cent of cases.Reference Castillo, Pantanowitz and Dezube 3 A potential association between plasmablastic lymphoma and human herpes virus 8 (Kaposi sarcoma herpes virus) was initially suspected due to confusion between plasmablastic lymphoma and the now recognised, separate entity of multicentric Castleman's disease, which contains Kaposi sarcoma herpes virus; increasing evidence currently indicates that most plasmablastic lymphomata do not contain this virus.Reference Carbone and Gloghini 10

Case reports

Case one

A 49-year-old, HIV-positive man presented with vocal change and odynophagia of two months' duration, together with a one-month history of multiple small, subcutaneous, nodular masses on the anterior chest and abdominal wall. He had been receiving HAART for six months and anti-tuberculosis treatment for five months. His absolute cluster of differentiation 4 glycoprotein (‘CD4’) level was 49 × 106/l, and his erythrocyte sedimentation rate (ESR) was 126 mm/hour.

Clinical evaluation revealed an underweight man with a muffled voice. Signs of upper airway obstruction were not present. At flexible nasendoscopy, a bulky, exophytic lesion of the supraglottic larynx was found, with pooled salivary secretions (Figure 1). The ENT examination was otherwise unremarkable, with no cervical lymphadenopathy of note. Further examination revealed multiple firm, non-tender, subcutaneous masses ranging from 1 to 3 cm in greatest dimension and extending across the anterior chest and abdominal wall (Figure 2).

Fig. 1

Nasendoscopic photograph of supraglottis of case one.

Fig. 2

Clinical photograph of anterior chest and abdomen of case one.

The laryngeal mass was biopsied under local anaesthesia. Fine needle aspiration of the anterior abdominal wall and chest masses revealed an atypical lymphoid population which was suspicious for lymphoma. Excision biopsy of a subcutaneous nodule was subsequently performed under local anaesthesia. Histological sections of the laryngeal mass and subcutaneous nodule showed similar features, i.e. solid, diffuse infiltrates of large, round to oval, neoplastic cells with a paranuclear hof imparting a plasmacytoid appearance. The nuclear chromatin was coarse and the nucleolus prominent. There were frequent mitotic figures. The significant immunohistochemical results were negativity for cluster of differentiation (CD) 20 protein and positivity for plasma cell markers (i.e. CD 138 protein and MUM1 protein), an immunoprofile consistent with plasmablastic lymphoma (Table I).

Table I

Immunohistochemistry

* Also known as leukocyte common antigen. CD = cluster of differentiation (glyco)protein; += positive; – = negative; ND = not done; EMA = epithelial membrane antigen; EBV LMP1 = Epstein–Barr virus latent membrane protein 1

Case two

A 41-year-old, HIV-positive woman receiving HAART presented with a 9-month history of vocal change, dysphagia and odynophagia. The patient had a past history of pulmonary tuberculosis (TB). Severe upper airway obstruction necessitated an emergency tracheostomy under local anaesthesia. She had an absolute CD4 level of 57 × 106/l and an ESR of 126 mm/hour, in addition to macrocytic anaemia.

At endoscopy, we found synchronous tumours of the supraglottic larynx (involving the laryngeal surface of the epiglottis) and the post-nasal space. Biopsy of both tumours revealed neoplastic infiltrates composed of large, round to oval cells with amphophilic cytoplasm and a discernible paranuclear hof in some cells (Figure 3a). Nuclei had stippled chromatin and the nucleolus was prominent. The neoplastic cells were positive for CD 138 protein (Figure 3b), MUM1 protein and VS38c but negative for CD 20 protein, an immunoprofile consistent with plasmablastic lymphoma.

Fig. 3

Photomicrographs for case two showing: (a) diffuse infiltration of round neoplastic cells with eccentric nuclei and paranuclear hofs (H&E; × 20); and (b) neoplastic cells with diffuse membrane immunoreactivity for CD 138 protein (×).

Both patients were treated with cyclophosphamide, doxorubicin, vincristine and prednisone (the ‘CHOP’ regimen), and their HAART was also continued.

Both patients were alive one year after diagnosis of plasmablastic lymphoma.

Discussion

Clinicians and pathologists should have a high degree of awareness of plasmablastic lymphoma occurring in unusual sites in HIV-infected individuals. The cases described above demonstrate that plasmablastic lymphoma is not necessarily confined to the oral and nasal cavities but may occur lower in the respiratory tract.

Clinical and microscopic features may not be sufficient to distinguish plasmablastic lymphoma from other malignancies of the head and neck such as undifferentiated carcinoma, other lymphoproliferative diseases, and malignant melanoma. Infective pathology, such as TB, is an important clinical differential diagnosis, particularly in regions with a high prevalence of TB and HIV.

Biopsy with detailed histopathological and immunohistochemical evaluation is recommended to ensure correct diagnosis and optimal management. Microscopically, plasmablastic lymphoma may resemble diffuse large B-cell lymphoma with immunoblastic morphology; however, in this situation testing for CD 20 protein would be positive. In some cases, plasmablastic lymphoma may resemble undifferentiated carcinoma and melanoma, but in both these instances immunohistochemistry is a very useful means of arriving at the correct diagnosis. Epstein–Barr virus has been demonstrated in plasmablastic lymphoma by Epstein–Barr virus encoded RNA in situ hybridisation; however, immunohistochemical detection of Epstein–Barr virus latent membrane protein 1 is usually negative.Reference Dong, Scadden, de Leval, Tang, Isaacson and Harris 11

  • • Plasmablastic lymphoma is a rare, aggressive form of non-Hodgkin's lymphoma

  • • A strong association with immunosuppression exists

  • • Most tumours are oral, but may also be extra-oral

  • • Laryngeal tumours may occur, with upper airway obstruction

  • • Detailed histopathological and immunohistochemical evaluation is required

Optimal treatment of plasmablastic lymphoma has not yet been clearly defined. Chemotherapy has been the mainstay of treatment in the vast majority of cases reported.Reference Castillo and Reagan 2 The most commonly used regimen is a combination of cyclophosphamide, doxorubicin, vincristine and prednisone; however more intensive regimes have also been explored.Reference Castillo, Winer, Stachurski, Perez, Jabbour and Milani 12 The relapse rate is high and overall survival rates are poor: a median overall survival of 14 months with a 5-year overall survival rate of 31 per cent have been estimated.Reference Carbone 5 It is notable that these data reflect a variety of therapeutic approaches in a heterogeneous patient population. Furthermore, an early meta-analysis has suggested that HAART in addition to chemotherapy and/or radiotherapy may improve prognosis and survival.Reference Guan, Zhang, Ma, Zhou and Zhou 13

Conclusion

When evaluating head and neck mucosal lesions, plasmablastic lymphoma and other non-Hodgkin's lymphomata are important differential diagnoses to be considered, particularly in the setting of a high prevalence of HIV.

References

1 Delecluse, HJ, Anagnostopoulos, I, Dallenbach, F, Hummel, M, Marafioti, T, Schneider, U et al. Plasmablastic lymphomas of the oral cavity: a new entity associated with the human immunodeficiency virus infection. Blood 1997;89:1413–20CrossRefGoogle ScholarPubMed
2 Castillo, JJ, Reagan, JL. Plasmablastic lymphoma: a systematic review. Scientific World Journal 2011;11:687–96CrossRefGoogle ScholarPubMed
3 Castillo, J, Pantanowitz, L, Dezube, B. HIV-associated plasmablastic lymphoma: lessons learned from 112 published cases. Am J Hematology 2008;83:804–9CrossRefGoogle ScholarPubMed
4 Castillo, JJ, Winer, ES, Stachurski, D, Perez, K, Jabbour, M, Milani, C et al. Clinical and pathological differences between human immunodeficiency virus-positive and human immunodeficiency virus-negative patients with plasmablastic lymphoma. Leuk Lymphoma 2010;51:2047–53Google Scholar
5 Carbone, A. AIDS-related non-Hodgkin's lymphomas: from pathology and molecular pathogenesis to treatment. Hum Pathol 2002;33:392–404 Google Scholar
6 Rafaniello Raviele, P, Pruneri, G, Maiorano, E. Plasmablastic lymphoma: a review. Oral Diseases 2009;15:38–45 Google Scholar
7 Bohlius, J, Schmidlin, K, Costagliola, D, Fätkenheuer, G, May, M, Caro-Murillo, AM et al. Incidence and risk factors of HIV-related non-Hodgkin's lymphoma in the era of combination antiretroviral therapy: a European multicohort study. Antivir Ther 2009;14:1065–74Google Scholar
8 Thirlwell, C, Sarker, D, Stebbing, J, Bower, M. Acquired immunodeficiency syndrome-related lymphoma in the era of highly active antiretroviral therapy. Clin Lymphoma 2003;4:86–92 Google Scholar
9 Carbone, A, Cesarman, E, Spina, M, Gloghini, A, Schulz, TF. HIV-associated lymphomas and gamma-herpesviruses. Blood 2009;113:1213–24CrossRefGoogle ScholarPubMed
10 Carbone, A, Gloghini, A. Plasmablastic lymphoma: one or more? Am J Hematol 2008;83:763–4CrossRefGoogle ScholarPubMed
11 Dong, HY, Scadden, DT, de Leval, L, Tang, Z, Isaacson, PG, Harris, NL. Plasmablastic lymphoma in HIV-positive patients: an aggressive Epstein-Barr virus-associated extramedullary plasmacytic neoplasm. Am J Surg Pathol 2005;29:1633–41Google Scholar
12 Castillo, JJ, Winer, ES, Stachurski, D, Perez, K, Jabbour, M, Milani, C et al. Prognostic factors in chemotherapy-treated patients with HIV-associated plasmablastic lymphoma. Oncologist 2010;15:293–9CrossRefGoogle ScholarPubMed
13 Guan, B, Zhang, X, Ma, H, Zhou, H, Zhou, X. A meta-analysis of highly active anti-retroviral therapy for treatment of plasmablastic lymphoma. Ann Saudi Med 2010;30:123–8Google Scholar
Figure 0

Fig. 1 Nasendoscopic photograph of supraglottis of case one.

Figure 1

Fig. 2 Clinical photograph of anterior chest and abdomen of case one.

Figure 2

Table I Immunohistochemistry

Figure 3

Fig. 3 Photomicrographs for case two showing: (a) diffuse infiltration of round neoplastic cells with eccentric nuclei and paranuclear hofs (H&E; × 20); and (b) neoplastic cells with diffuse membrane immunoreactivity for CD 138 protein (×).