Hostname: page-component-76fb5796d-r6qrq Total loading time: 0 Render date: 2024-04-25T19:48:22.656Z Has data issue: false hasContentIssue false
  • English
  • Français

Protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion

Published online by Cambridge University Press:  16 November 2010

S-Q Zhai ,
W Guo ,
Y-Y Hu ,
N Yu ,
Q Chen ,
J-Z Wang ,
M Fan  and
W-Y Yang
S-Q Zhai*
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
W Guo
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
Y-Y Hu
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
N Yu
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
Q Chen
Affiliation:
Institute of Basic Medical Science, Academy of Military Medical Sciences of People's Liberation Army, Beijing, China
J-Z Wang
Affiliation:
Institute of Basic Medical Science, Academy of Military Medical Sciences of People's Liberation Army, Beijing, China
M Fan
Affiliation:
Institute of Basic Medical Science, Academy of Military Medical Sciences of People's Liberation Army, Beijing, China
W-Y Yang
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
*
Address for correspondence: Dr Suo-Qiang Zhai, 28 Fuxing Road, Haidian District, Beijing 100853, China Fax: 86 024 23380681 E-mail: gaochunyan1954@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

To explore the protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion.

Methods:

Recombinant adenovirus brain-derived neurotrophic factor vector, recombinant adenovirus LacZ and artificial perilymph were prepared. Guinea pigs with audiometric auditory brainstem response thresholds of more than 75 dB SPL, measured seven days after four hours of noise exposure at 135 dB SPL, were divided into three groups. Adenovirus brain-derived neurotrophic factor vector, adenovirus LacZ and perilymph were infused into the cochleae of the three groups, variously. Eight weeks later, the cochleae were stained immunohistochemically and the spiral ganglion cells counted.

Results:

The auditory brainstem response threshold recorded before and seven days after noise exposure did not differ significantly between the three groups. However, eight weeks after cochlear perfusion, the group receiving brain-derived neurotrophic factor had a significantly decreased auditory brainstem response threshold and increased spiral ganglion cell count, compared with the adenovirus LacZ and perilymph groups.

Conclusion:

When administered via cochlear infusion following noise damage, brain-derived neurotrophic factor appears to improve the auditory threshold, and to have a protective effect on the spiral ganglion cells.

Keywords

CochleaNoise-Induced Hearing LossBrain-Derived Neurotrophic FactorGuinea Pig
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 125 , Issue 5 , May 2011 , pp. 449 - 454
Copyright
Copyright © JLO (1984) Limited 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Rejali, D, Lee, VA, Abrashkin, KA, Humayun, N, Swiderski, DL, Raphael, Y. Cochlear implants and ex vivo BDNF gene therapy protect spiral ganglion neurons. Hear Res 2007;228:180–7CrossRefGoogle ScholarPubMed
2Miller, JM, Le Prell, CG, Prieskorn, DM, Wys, NL, Altschuler, RA. Delayed neurotrophin treatment following deafness rescues spiral ganglion cells from death and promotes regrowth of auditory nerve peripheral processes: effects of brain-derived neurotrophic factor and fibroblast growth factor. J Neurosci Res 2007;85:1959–6CrossRefGoogle ScholarPubMed
3Pirvola, U, Ylikoski, J, Palgi, J, Lehtonen, E, Arumae, U, Saarma, M. Brain-derived neurotrophic factor and neurotrophin 3 mRNAs in the peripheral target fields of developing inner ear ganglia. Proc Natl Acad Sci U S A 1992;89:9915–19CrossRefGoogle ScholarPubMed
4Wise, AK, Richardson, R, Hardman, J, Clark, G, O'Leary, S. Resprouting and survival of guinea pig cochlear neurons in response to the administration of the neurotrophins brain-derived neurotrophic factor and neurotrophin-3. J Com Neurol 2005;487:147–65CrossRefGoogle Scholar
5Nakaizumi, T, Kawamoto, K, Minoda, R, Raphael, Y. Adenovirus-mediated expression of brain-derived neurotrophic factor protects spiral ganglion neurons from ototoxic damage. Audiol Neurootol 2004;9:135–43CrossRefGoogle ScholarPubMed
6Chen, Q, Wang, JZ, Liu, H, Wu, Y, Fan, M. Construction and characterization of recombinant adenoviruses expressing biologically active human brain-derived neurotrophic factor and neurotrophin-3. Acta Biochim Biophys Sin 2000;32:121–5Google ScholarPubMed
7Fang, YY, Yao, XQ, Jiang, SC, Wang, PY, Sun, JH, Li, Y. A quantitive study on the cochlea hair cell and spiral ganglion cell in senile guinea pigs. Chin J Stereo Image Anal 1996;1:64–7Google Scholar
8Agterberg, MJ, Versnel, H, de Groot, JC, Smoorenburg, GF, Albers, FW, Klis, SF. Morphological changes in spiral ganglion cells after intracochlear application of brain-derived neurotrophic factor in deafened guinea pigs. Hear Res 2008;244:25–34CrossRefGoogle ScholarPubMed
9Lefebvre, P, Weber, T, Rigo, JM, Staecker, H, Moonen, G, Van De Water, TR. Peripheral and central target-derived trophic factor(s) effects on auditory neurons. Hear Res 1992;58:185–92CrossRefGoogle ScholarPubMed
10Rubel, EW, Fritzsch, B. Auditory system development: primary auditory neurons and their targets. Annu Rev Neurosci 2002;25:51–101CrossRefGoogle ScholarPubMed
11Ylikoski, J, Pirvola, U, Virkkala, J, Suvanto, P, Liang, XQ, Magal, E et al. Guinea pig auditory neurons are protected by glial cell line-derived growth factor from degeneration after noise trauma. Hear Res 1998;124:17–26CrossRefGoogle ScholarPubMed
12Kanzaki, S, Stover, T, Kawamoto, K, Prieskorn, DM, Altschuler, RA, Miller, JM et al. Glial cell line-derived neurotrophic factor and chronic electrical stimulation prevent VIII cranial nerve degeneration following denervation. J Comp Neurol 2002;454:350–60CrossRefGoogle ScholarPubMed
13Ylikoski, JPirvola, U, Moshnyakov, M, Palgi, J, Arumäe, U, Saarma, M. Expression patterns of neurotrophin and their receptor mRNAs in the rat inner ear. Hear Res 1993;65:69–78CrossRefGoogle ScholarPubMed
14Lefebvre, PP, Malgrange, B, Staecker, H, Moghadass, M, Van de Water, TR, Moonen, G. Neurotrophins affect survival and neuritogenesis by adult injured auditory neurons in vitro. Neuroreport 1994;5:865–8CrossRefGoogle ScholarPubMed
15Shepherd, RK, Coco, A, Epp, SB, Crook, JM. Chronic depolarization enhances the trophic effects of brain-derived neurotrophic factor in rescuing auditory neurons following a sensorineural hearing loss. J Comp Neurol 2005;486:145–58CrossRefGoogle ScholarPubMed
16Lu, B. BDNF and activity-dependent synaptic modulation. Learn Mem 2003;10:86–98CrossRefGoogle ScholarPubMed
17Du, J, Feng, L, Yang, F, Lu, B. Activity- and Ca(2+)-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons. J Cell Biol 2000;150:1423–34CrossRefGoogle ScholarPubMed
18Davis, RL. Gradients of neurotrophins, ion channels, and tuning in the cochlea. Neuroscientist 2003;9:311–16CrossRefGoogle ScholarPubMed
19Bok, J, Zha, XM, Cho, YS, Green, SH. An extranuclear locus of cAMP-dependent protein kinase action is necessary and sufficient for promotion of spiral ganglion neuronal survival by cAMP. J Neurosci 2003;23:777–87CrossRefGoogle ScholarPubMed
20Balkowiec, A, Katz, DM. Cellular mechanisms regulating activity-dependent release of native brain-derived neurotrophic factor from hippocampal neurons. J Neurosci 2002;22:10399–407CrossRefGoogle ScholarPubMed
21Desagher, S, Martinou, JC. Mitochondria as the central control point of apoptosis. Trends Cell Biol 2000;10:369–77CrossRefGoogle ScholarPubMed