Hostname: page-component-76d6cb85b7-6jg5l Total loading time: 0 Render date: 2026-07-18T04:44:02.246Z Has data issue: false hasContentIssue false
  • English
  • Français

Genotypic variation in callus induction and plant regeneration of Saccharum barberi

Published online by Cambridge University Press:  16 September 2025

Min Zhang ,
Jun Mao ,
Xin Lu ,
Xujuan Li ,
Chunyan Tian ,
Xin Hu ,
Chunjia Li  and
Luodong Huang Open the ORCID record for Luodong Huang [Opens in a new window]
Min Zhang
Affiliation:
College of Life Science and Technology, Guangxi University, Nanning, China National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Jun Mao
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Xin Lu
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Xujuan Li
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Chunyan Tian
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Xin Hu
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Chunjia Li*
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Luodong Huang*
Affiliation:
College of Life Science and Technology, Guangxi University, Nanning, China
*
Corresponding author: Luodong Huang; Email: ynhuangld@gxu.edu.cn; Chunjia Li; Email: lcjzt@163.com
Corresponding author: Luodong Huang; Email: ynhuangld@gxu.edu.cn; Chunjia Li; Email: lcjzt@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Saccharum barberi is regarded as a sugarcane germ plasm resource of potential value. Tissue culture serves multiple purposes in breeding-related research for sugarcane. The response to tissue culture varies considerably among sugarcane genotypes; however, the influence of genetic differences on the tissue culture performance of S. barberi had not been previously investigated. This study evaluated the genotypic variation in tissue culture response among six accessions of S. barberi. Seven parameters were assessed to determine the tissue culture performance: callus induction frequency (CIF), embryogenic callus ratio, embryogenic callus induction frequency, callus regeneration frequency, callus regeneration coefficient, overall regeneration frequency (ORF) and overall regeneration coefficient (ORC). Significant variations (P < 0.05) were observed among the S. barberi genotypes for all parameters. The broad-sense heritability ranged from 80.77% to 93.10%, indicating that genetic differences were the primary source of genotypic variation. ORF exhibited the highest diversity among the parameters, with a genotypic coefficient of variation up to 70.06%. Pansahi was identified as the most amenable genotype to tissue culture, demonstrating superior performance in both callus induction and plant regeneration. CIFs at different induction periods were strongly positively correlated with both ORF and ORC, particularly during the first week, suggesting that CIF may serve as a promising early predictor of overall regeneration competence. This study is the first to report the effect of genotypic variation on callus induction and plant regeneration of S. barberi, and the findings will be valuable for future research involving tissue culture in this species.

Keywords

callus inductiongenotypic variationplant regenerationSaccharum barberisugarcane

Information

Type
Research Article
Information
Plant Genetic Resources , Volume 24 , Issue 1 , February 2026 , pp. 38 - 46
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany.

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Abu, G, Mekbib, F and Teklewold, A (2014) Effect of genotype on in vitro propagation of elite sugarcane (Saccharum officinarum L.) varieties of Ethiopian sugar estates. International Journal of Technology Enhancements and Emerging Engineering Research 2, 123–128.Google Scholar
Adhikari, PB, Yoon, CS and Kang, WH (2016) Callus induction and shoot regeneration of commercial tomatoes (Solanum lycopersicum) through anther culture. Acta Horticulturae 1142, 389–394.CrossRefGoogle Scholar
Basnayake, SW, Moyle, R and Birch, RG (2011) Embryogenic callus proliferation and regeneration conditions for genetic transformation of diverse sugarcane cultivars. Plant Cell Reports 30, 439–448.CrossRefGoogle ScholarPubMed
Basso, MF, da Cunha, BADB, Ribeiro, AP, Martins, PK, de Souza, WR, de Oliveira Ng, , Nakayama, TJ, Das Chagas Noqueli Casari, RA, Santiago, TR, Vinecky, F, Cancado, LJ, de Sousa, CAF, de Oliveira, PA, de Souza, SACD, de Almeida Cancado, GM, Kobayashi, AK and Molinari, HBC (2017) Improved genetic transformation of sugarcane (Saccharum spp.) embryogenic callus mediated by Agrobacterium tumefaciens. Current Protocols in Plant Biology 2, 221–239.CrossRefGoogle ScholarPubMed
Birch, RG (2014) Sugarcane biotechnology: Axenic culture, gene transfer, and transgene expression. In Moore, PH and Botha, FC (eds), Sugarcane: physiology, Biochemistry, and Functional Biology. New Jersey: John Wiley & Sons, pp. 645–681.Google Scholar
Brant, EJ, Eid, A, Kannan, B, Baloglu, MC and Altpeter, F (2024) The extent of multiallelic, co-editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield. Plant Biotechnology Journal 22, 2660–2671.CrossRefGoogle ScholarPubMed
Brown, JS, Schnell, RJ, Power, EJ, Douglas, SL and Kuhn, DN (2007) Analysis of clonal germplasm from five Saccharum species: S. barberi, S. robustum, S. officinarum, S. sinense and S. spontaneum. A study of inter-and intraspecies relationships using microsatellite markers. Genetic Resources and Crop Evolution 54, 627–648.CrossRefGoogle Scholar
Chandran, K (2010) In vitro multiplication and conservation of Saccharum germplasm. Indian Journal of Plant Genetic Resources 23, 65–68.Google Scholar
Daniels, C and Daniels, J (1975) The geographical, historical, and cultural aspects of the origin of Indian and Chinese sugarcanes, S. barberi and S. sinense. International Journal of Sugar Cane Breeders’ Newsletter 36, 4–123.Google Scholar
Dewanti, P, Widuri, LI, Ainiyati, C, Okviandari, P, Maisaro, and Sugiharto, B (2016) Elimination of SCMV (sugarcane Mozaik virus) and rapid propagation of virus-free sugarcane (Saccharum officinarum L.) using somatic embryogenesis. Procedia Chemistry 18, 96–102.CrossRefGoogle Scholar
D’Hont, A, Ison, D, Alix, K, Roux, C and Glaszmann, JC (1998) Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes. Genome 41, 221–225.CrossRefGoogle Scholar
D’hont, A, Paulet, F and Glaszmann, JC (2002) Oligoclonal interspecific origin of ‘North Indian’and ‘Chinese’sugarcanes. Chromosome Research 10, 253–262.CrossRefGoogle ScholarPubMed
Falconer, DS (1989) Introduction to Quantitative Genetics, 3rd Edn. New York: Longman.Google Scholar
Geijskes, RJ, Wang, L, Lakshmanan, P, Mckeon, MG, Berding, N, Swain, RS, Elliott, AR, Grof, CPL, Jackson, JA and Smith, GR (2003) SmartsettTM seedlings: Tissue cultured seed plants for the Australian sugar industry. Sugarcane International, 13–17. doi: 10.5555/20033099958.Google Scholar
Grogg, D, Rohner, M, Yates, S, Manzanares, C, Bull, SE, Dalton, S, Bosch, M, Studer, B and Broggini, GAL (2022) Callus induction from diverse explants and genotypes enables robust transformation of perennial ryegrass (Lolium perenne L.). Plants 11, 2054.CrossRefGoogle ScholarPubMed
Guo, Y, Sun, S, Chen, S, Zhang, Y, Wang, W, Deng, Z, Liu, Z, Yu, Z, Xu, H, Guo, J, Zhang, S, Zhong, Y, Zhang, W, Chen, J, Zhou, F, Chang, H, Gao, S and Wang, Q (2024) In vivo induction of sugarcane (Saccharum spp.) haploids by genome editing. Plant Physiology 196, 731–734.CrossRefGoogle ScholarPubMed
Gupta, V and Sharma, S (2024) Advancements in Sugarcane Tissue Culture: A Review. Agricultural Reviews Advance online publication. doi: 10.18805/ag.R-2723.CrossRefGoogle Scholar
Han, Y, Jin, XL, Wu, FB and Zhang, GP (2011) Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.). Journal of Zhejiang University Science B 12, 399–407.CrossRefGoogle ScholarPubMed
Kaur, A and Sandhu, JS (2015) High throughput in vitro micropropagation of sugarcane (Saccharum officinarum L.) from spindle leaf roll segments: Cost analysis for agri-business industry. Plant Cell Tissue and Organ Culture 120, 339–350.CrossRefGoogle Scholar
Khvatkov, P, Chernobrovkina, M, Okuneva, A, Shvedova, A, Chaban, I and Dolgov, S (2015) Callus induction and regeneration in Wolffia arrhiza (L.) Horkel ex Wimm. Plant Cell Tissue and Organ Culture 120, 263–273.CrossRefGoogle Scholar
Kumar, T, Wang, JG, Xu, CH, Lu, X, Mao, J, Lin, XQ, Kong, CY, Li, CJ, Li, XJ, Tian, CY, Ebid, MHM, Liu, XL and Liu, HB (2024) Genetic engineering for enhancing sugarcane tolerance to biotic and abiotic stresses. Plants 13, 1739.CrossRefGoogle ScholarPubMed
Li, CJ, Li, XJ, Lin, XQ, Qin, W, Lu, X, Mao, J and Liu, XL (2021) Genotypic variation in the response to embryogenic callus induction and regeneration in Saccharum spontaneum. Plant Genetic Resources: Characterization and Utilization 19, 153–158.CrossRefGoogle Scholar
Li, SC, Duan, WX, Zhao, JH, Jing, YF, Feng, MF, Kuang, BW, Wei, N, Chen, BS and Yang, XP (2022) Comparative analysis of chloroplast genome in Saccharum spp. and related members of ‘Saccharum Complex’. International Journal of Molecular Sciences 23, 7661.CrossRefGoogle ScholarPubMed
Lu, G, Wang, Z, Xu, F, Pan, YB, Grisham, MP and Xu, L (2021) Sugarcane mosaic disease: Characteristics, identification and control. Microorganisms 9, 1984.CrossRefGoogle ScholarPubMed
Masoabi, M, Lloyd, J, Kossmann, J and Van der Vyver, C (2018) Ethyl methanesulfonate mutagenesis and in vitro polyethylene glycol selection for drought tolerance in sugarcane (Saccharum spp.). Sugar Technology 20, 50–59.CrossRefGoogle Scholar
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.CrossRefGoogle Scholar
Nogueira, GF, Luis, ZG, Pasqual, M and Scherwinski-Pereira, JE (2019) High-efficiency somatic embryogenesis of a broad range of Brazilian Saccharum spp. hybrids (sugarcane) varieties using explants from previously established in vitro plants. Vitro Cellular & Developmental Biology-Plant 55, 26–35.CrossRefGoogle Scholar
Pan, YB, Todd, JR, Lomax, L, White, PM Jr, Simpson, SA and Scheffler, BE (2023) Molecular Dissection of the 5S Ribosomal RNA-Intergenic Transcribed Spacers in Saccharum spp. and Tripidium spp. Agronomy 13, 2728.CrossRefGoogle Scholar
Patel, VS, Mehta, R, Naik, KH, Singh, D, Patel, DU and Mali, SC (2015) Callus induction & whole plant regeneration in sugarcane (Saccharum spp. complex) variety Co 86032. Green Farming 6, 935–939.Google Scholar
Pompidor, N, Charron, C, Hervouet, C, Bocs, S, Droc, G, Rivallan, R, Manez, A, Mitros, T, Swaminathan, K, Glaszmann, JC, Garsmeur, O and D’Hont, A (2021) Three founding ancestral genomes involved in the origin of sugarcane. Annals of Botany 127, 827–840.CrossRefGoogle ScholarPubMed
Ram, B and Hemaprabha, G (1998) Nature and pattern of genetic divergence of sugar yield and its components in the progenies of Saccharum barberi. Indian Journal of Genetics and Plant Breeding 58, 193–199.Google Scholar
Rao, S and Jabeen, FTZ (2013) Optimization of protocols for callus induction, regeneration and acclimatization of sugarcane (Saccharum officinarum L.) cultivar CO-86032. Current Trends in Biotechnology and Pharmacy 7, 862–869.Google Scholar
Reed, S, Ayala‐Silva, T, Brown, S, Glaz, B and Comstock, J (2012) Screening Saccharum barberi and sinense accessions for flood tolerance and biomass production. Journal of Agronomy and Crop Science 198, 236–244.CrossRefGoogle Scholar
Schmidt, P, Hartung, J, Rath, J and Piepho, HP (2019) Estimating broad‐sense heritability with unbalanced data from agricultural cultivar trials. Crop Science 59, 525–536.CrossRefGoogle Scholar
Snyman, SJ, Meyer, GM, Richards, JM, Haricharan, N, Ramgareeb, S and Hucket, BI (2006) Refining the application of direct embryogenesis in sugarcane: Effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency. Plant Cell Reports 25, 1016–1023.CrossRefGoogle ScholarPubMed
Tai, PYP and Miller, JD (2001) A core collection for Saccharum spontaneum L. from the world collection of sugarcane. Crop Science 41, 879–885.CrossRefGoogle Scholar
Taylor, PW, Ko, HL, Adkins, SW, Rathus, C and Birch, RG (1992) Establishment of embryogenic callus and high protoplast yielding suspension cultures of sugarcane (Saccharum spp. hybrids). Plant Cell Tissue and Organ Culture 28, 69–78.CrossRefGoogle Scholar
Türkoğlu, A, Bolouri, P, Haliloğlu, K, Eren, B, Demirel, F, Işık, Mİ, Piekutowska, M, Wojciechowski, T and Niedbała, G (2023) Modeling callus induction and regeneration in hypocotyl explant of fodder pea (Pisum sativum var. arvense L.) using machine learning algorithm method. Agronomy 13, 2835.CrossRefGoogle Scholar
Wang, WZ, Yang, BP, Feng, XY, Cao, ZY, Feng, CL, Wang, JG, Xiong, GR, Shen, LB, Zeng, J, Zhao, TT and Zhang, SZ (2017) Development and characterization of transgenic sugarcane with insect resistance and herbicide tolerance. Frontiers in Plant Science 8, 1535.CrossRefGoogle ScholarPubMed
Yadav, S, Ahmad, A, Rastogi, J and Lal, M (2012) Tissue culture strategies in sugarcane (Saccharum officinarum L.). International Journal of Pharma and Bio Sciences 3, 426–441.Google Scholar
Yang, WT, Zhai, HW, Wu, FM, Deng, L, Chao, Y, Meng, XW, Chen, Q, Liu, CH, Bie, XM, Sun, CL, Yu, Y, Zhang, XF, Zhang, XY, Chang, ZQ, Xue, M, Zhao, YJ, Meng, XB, Li, BS, Zhang, XS, Zhang, DJ, Zhao, XY, Gao, CX, Li, JY and Li, CY (2024) Peptide REF1 is a local wound signal promoting plant regeneration. Cell 187, 3024–3038.CrossRefGoogle ScholarPubMed
Zaman, S, Erum, S, Javed, H, Khan, S and Kanwal, S (2014) In-vitro conservation of sugarcane germplasm. Pakistan Journal of Agricultural Research 36, 549–556.Google Scholar
Supplementary material: File

Zhang et al. supplementary material

Zhang et al. supplementary material
Download Zhang et al. supplementary material(File)
File 717.7 KB