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Morphological evidence of biofilm in chronic obstructive sialadenitis

Published online by Cambridge University Press:  10 July 2018

S A Schrøder ,
S Eickhardt ,
T Bjarnsholt ,
T Nørgaard  and
P Homøe
S A Schrøder*
Affiliation:
Department of Otorhinolaryngology, Nordsjaellands University Hospital, Hillerød, Denmark Department of Otorhinolaryngology Head and Neck Surgery, Rigshospitalet – Copenhagen University Hospital, University of Copenhagen, Denmark
S Eickhardt
Affiliation:
Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
T Bjarnsholt
Affiliation:
Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark Department of Clinical Microbiology, Copenhagen University Hospital, Denmark
T Nørgaard
Affiliation:
Department of Pathology, Rigshospitalet – Copenhagen University Hospital, University of Copenhagen, Denmark
P Homøe
Affiliation:
Department of Otorhinolaryngology and Maxillofacial Surgery, Zealand University Hospital, Køge, Denmark
*
Address for correspondence: Dr Stine A Schrøder, Department of Otorhinolaryngology, Nordsjaellands University Hospital, Hillerød, Denmark Fax: +45 4829 3811 E-mail: stine.eva.attrup.schroeder@regionh.dk
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Abstract

Objective

Bacterial infection is a common finding in acute sialadenitis and may play a role in the chronicity of the condition. This study investigated if bacterial biofilm is present in submandibular chronic obstructive sialadenitis.

Methods

A descriptive case–control study was conducted that compared 10 histological sections of submandibular glands with chronic obstructive sialadenitis, to 10 histological sections of the healthy part of submandibular glands with pleomorphic adenoma. Fluorescence in situ hybridisation and confocal laser scanning microscopy visualised evidence of bacterial biofilm.

Results

In the chronic obstructive sialadenitis group, 5 out of 10 histological sections showed morphological evidence of bacterial biofilm. In the control group, there was no sign of bacterial biofilm formation.

Conclusion

Morphological evidence of bacterial biofilm was found in the submandibular gland sections from patients with chronic sialadenitis and suggests a role in the chronicity of submandibular chronic obstructive sialadenitis.

Keywords

BacteriaBiofilmsSalivary Gland CalculiSialadenitis

Information

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 132 , Issue 7 , July 2018 , pp. 611 - 614
Copyright
Copyright © JLO (1984) Limited, 2018 

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Footnotes

Dr S A Schrøder takes responsibility for the integrity of the content of the paper

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