Management Implications
Results of a reseeding experiment suggest that diverse native seed mixes can be a valuable tool for the restoration of productivity and function to ecosystems previously infested by Vincetoxicum rossicum (pale swallow-wort). Biomass produced by these native seed mixes was dominated by grass species, including big bluestem, Andropogon gerardii Vitman, Canada wild rye, Elymus canadensis L., bottlebrush grass, Elymus hystrix L., and Virginia wildrye, Elymus virginicus L., and the nurse crop Italian ryegrass, Lolium perenne L. spp. multiflorum. Based on these results, we advocate for the inclusion of these species in seed mixes used for post–V. rossicum restoration. In contrast, use of Asclepias syriaca (common milkweed) should be carefully considered for restoration of invaded sites, because the plant can have negative effects on native seed germination and can promote V. rossicum regrowth from rootstock.
Additionally, results from germination and transplantation experiments suggest that following removal of V. rossicum from a site, residual seeds of the weed likely play a secondary role in the resurgence of the invasive species, as germination is inhibited in V. rossicum–conditioned soil. The rapid resurgence of the weed, often observed after control, is most likely driven by V. rossicum root crowns remaining in the soil. If these root crowns can be successfully removed from infestation sites, then the competitive advantage of the weed over native plants can be substantially reduced.
Introduction
Many invasive plants gain a foothold in new environments by manipulating soil conditions in their favor. Invasive weeds may undermine native plant growth by producing phytotoxic chemicals (allelopathy) (Bais et al. Reference Bais, Vepachedu, Gilroy, Callaway and Vivanco2003; Chen et al. Reference Chen, Liao, Chen, Wei and Peng2017), by disrupting mutualisms between native plants and their mycorrhizal associates (Van der Heijden et al. Reference Van der Heijden, Boller, Weimken and Sanders1998), or by sequestering soil pathogens on their roots (Mangla et al. Reference Mangla and Callaway2008). Such changes in soil conditions, particularly an altered rhizosphere, can have lasting impacts on the recovery of habitats even after the invasive weed has been removed. We conducted a series of greenhouse experiments, examining the effects of soil conditioned by pale swallow-wort [Vincetoxicum rossicum (Kleopow) Barbarich; Apocynaceae] on the growth of native plants. We sought to generate a clearer understanding of how V. rossicum–conditioned soil affects native plant growth and to provide direction for the restoration of invaded sites.
Vincetoxicum rossicum is a perennial vine native to southwestern Ukraine (Pobedimova Reference Pobedimova1952). The species was introduced to North America in the 1800s and has since become highly invasive in Ontario and Quebec, Canada, as well as in the northeastern United States (DiTommaso et al. Reference DiTommaso, Lawlor and Darbyshire2005; Douglass et al. Reference Douglass, Weston and DiTommaso2009). The weed reduces native plant diversity by smothering neighboring vegetation (Christensen Reference Christensen1998), and the displacement of native plants has cascading effects on native arthropod assemblages (Ernst and Cappuccino Reference Ernst and Cappuccino2005). Among the insects affected by V. rossicum are monarch butterflies (Danaus plexippus L. (Lepidoptera: Nymphalidae). Monarchs regularly lay eggs on V. rossicum, a close relative of common milkweed (Asclepias syriaca L.; Apocynaceae), but the caterpillars cannot complete development on the weed (Casagrande and Dacey Reference Casagrande and Dacey2007). Invasive swallow-worts (V. rossicum and V. nigrum) have also been linked to reductions in breeding birds in grassland habitats (DiTommaso et al. Reference DiTommaso, Lawlor and Darbyshire2005) and, of particular concern in Canada, the weeds are encroaching on rare alvar communities in Ontario that are home to multiple species at risk (Lawlor Reference Lawlor2000).
Allelopathy has long been considered a “novel weapon” that enhances the competitive abilities of weed species in their introduced ranges (Callaway and Ridenour Reference Callaway and Ridenour2004). Many exotic plants are known to suppress native neighbors and facilitate their invasion by releasing chemicals into the environment (Hierro and Callaway Reference Hierro and Callaway2003). These chemicals often have stronger negative effects on plants that have not coevolved with the weed (Ridenour and Callaway Reference Ridenour and Callaway2001). Diffuse knapweed (Centaurea diffusa Lam.; Asteraceae), for example, has far greater allelopathic effects on plants in its invasive range (North America) than on plants in its native Europe (Callaway and Aschehoug Reference Callaway and Aschehoug2000). Vincetoxicum rossicum is known to produce allelochemicals, one of which (antofine) has been identified (Gibson et al. Reference Gibson, Krasnoff, Biazzo and Milbrath2011; Mogg et al. Reference Mogg, Petit, Cappuccino, Durst, McKague, Foster, Yack, Arnason and Smith2008). The role of allelopathy in V. rossicum invasions, however, is unclear. In agar bioassays, V. rossicum has been shown to inhibit germination in lettuce (Lactuca sativa L.) (Douglass et al. Reference Douglass, Weston and Wolfe2011) and reduce root growth in several native species, including A. syriaca (Gibson et al. Reference Gibson, Krasnoff, Biazzo and Milbrath2011). More recent studies by Gibson et al. (Reference Gibson, Vaughan and Milbrath2015), however, showed that antofine is unstable and is often absent from soil samples collected from mature V. rossicum infestations. Furthermore, dose–response experiments conducted in nonsterile soil showed that the concentration of antofine needed to reduce lettuce root growth was 20 to 50 times higher than concentrations used in previous agar bioassays.
In addition to allelopathy, invasive plants can manipulate soil conditions in their favor by altering rhizosphere communities (Hawkes et al. Reference Hawkes, Belnap, D’Antonio and Fire-stone2006; Mangla et al. Reference Mangla and Callaway2008; Stinson et al. Reference Stinson, Campbell, Powell, Wolfe, Callaway, Thelen, Hallett, Prati and Klironomos2006; Vogelsang and Bever Reference Vogelsang and Bever2009). Associations between plants and arbuscular mycorrhizal fungi (AMF) are often host specific (Appoloni et al. Reference Appoloni, Lekberg, Tercek, Zabinski and Redecker2008; Bever Reference Bever2004). As such, any disruption to fungal communities brought about by invasive plants can lead to fundamental changes in plant community structure (Vandenkoornhuyse Reference Vandenkoornhuyse, Husband, Daniell, Watson, Duck, Fitter and Young2002). Vincetoxicum rossicum infestations in Canada cause significant shifts in the AMF communities associated with native plant roots compared with those in neighboring noninvaded sites (Bongard et al. Reference Bongard, Navaranjan, Yan and Fulthorpe2013). Additionally, V. rossicum has been observed to accumulate pathogenic fungal partners that negatively affect native plant species (Day et al. Reference Day, Dunfield and Antunes2016; Dickinson et al. Reference Dickinson, Bourchier, Fulthorpe, Shen, Jones and Smith2021). As with allelopathy, however, the role of soil fungal manipulation in V. rossicum invasions is unclear. For example, Dukes et al. (Reference Dukes, Koyama, Dunfield and Antunes2019) showed that some native plants exhibited increased growth in V. rossicum–conditioned soil.
We conducted a series of four controlled greenhouse experiments designed to explore the effects of V. rossicum–conditioned soil on native plant growth and to identify effective practices for the restoration of native diversity after V. rossicum removal. First, we examined how soil from a mature V. rossicum infestation affected seedling emergence and establishment of both A. syriaca and V. rossicum. Second, we explored the effects of the same soil on mature A. syriaca plants. These first two experiments were designed to identify the life stage of representative native plants that are most vulnerable to changes in soil conditions elicited by V. rossicum. Third, we conducted a reseeding experiment to determine the success of two native seed mixes in soil conditioned by 13 wk of V. rossicum growth from seed. We sought to test the viability of reseeding schemes for the restoration of biodiversity and ecosystem function to V. rossicum–infested sites. Finally, we conducted a greenhouse experiment to determine the effects of aqueous A. syriaca extracts on the growth of V. rossicum from rootstock, to see whether A. syriaca, a native plant known to produce allelopathic chemicals (Rasmussen Reference Rasmussen1975), might represent a potential tool for the control of V. rossicum. With these experiments, we aimed to determine (1) how V. rossicum–conditioned soil affects native plant establishment, (2) whether native seed mixes are effective for restoring native vegetation to sites previously infested with V. rossicum, and (3) whether A. syriaca’s allelopathy could be used against V. rossicum in the context of ecological restoration.
Materials and Methods
Experiment 1: Assessing the Effects of Vincetoxicum rossicum–conditioned Soil on Seedling Emergence in Asclepias syriaca and Vincetoxicum rossicum
We examined how soil from a mature infestation of V. rossicum affects early plant establishment using A. syriaca and V. rossicum as indicator species. For this experiment, we used a one-way independent-samples design with two treatments: invaded versus uninvaded soil. Forest soil was collected from Uxbridge, ON (44.088694, −79.105694) within (treatment) or 10 m away from (control) a 15-yr-old V. rossicum infestation. Treatment and control soil was collected within and adjacent to the same V. rossicum infestation to ensure similarities in environmental and edaphic variables and land use history (Cahill et al. Reference Cahill, Cale, Karst, Bao, Pec and Erbilgin2016; Dukes et al. Reference Dukes, Koyama, Dunfield and Antunes2019; Karst et al. Reference Karst, Erbilgin, Pec, Cigan, Najar, Simard and Cahill2015). Collected soil was sieved through a 6-mm mesh and placed in 72-cell plug trays (50 ml per cell). Plug trays were watered for 1 wk, and any germinating seeds were removed by hand. After 1 wk, each cell was planted with a single V. rosscium seed (field collected from Uxbridge, ON, in February) or an A. syriaca seed (collected from the same field site in March). Seeds were all collected from the same site, in late winter, so that they had received natural cold stratification and their germinability would mirror that found in natural conditions. All seeds had been soaked overnight in 0.2% Nutriboost 1 (Nutrilife Plant Products, Abbotsford, BC, Canada) to improve their chances of germination. A total of 1,440 soil plugs were used, and 720 seeds of each species were planted. For each species, exactly half of the seeds were planted in treatment and control soil. Plug trays were placed in the University of Toronto forestry production greenhouse and watered as needed. Trays were randomly reconfigured weekly to control for any spatial differences in greenhouse conditions. Germination and death were recorded weekly for 4 wk, at which point above- and belowground biomass were separated and roots were washed. Roots and stems were oven-dried at 60 C for at least 3 d before weighing (Day et al. Reference Day, Antunes and Dunfield2015; Ernst and Cappuccino Reference Ernst and Cappuccino2005).
Experiment 2: Assessing the Effects of Vincetoxicum rossicum–conditioned Soil on Potted Asclepias syriaca plants
We compared the growth and performance of potted A. syriaca plants transplanted into soil from a 15-yr-old V. rossicum infestation and into soil from a nearby uninfested site. For this experiment, we used a one-way independent-samples design, comparing growth in infested versus uninfested soil. Treatment soil was collected from a V. rossicum infestation in Uxbridge, ON (44.088698, −79.105780), and control soil was collected from a site 10 m away from the V. rossicum infestation, as with Experiment 1. All soil was collected in July 2021. Soil was sieved as in Experiment 1 and stored in the greenhouse at the University of Toronto.
Asclepias syriaca plants were grown from seed (SKU: C11570; Wildflower Farm, Coldwater, ON, Canada). In May 2021, seeds were soaked as in Experiment 1, and planted individually in 0.5-L pots containing triple mix (Less Mess Products, Concord, ON, Canada). Seeds were watered as necessary, and pots were rotated weekly to control for any spatial variations in greenhouse conditions. On July 6, 2021, at 10 wk after planting, 92 A. syriaca plants were randomly selected, and transplanted into either V. rossicum–conditioned soil (46 plants) or control soil (46 plants). Immediately after transplanting, stem height was recorded for each experimental plant to ensure that plant size was relatively even between treatments. Plants were then watered as needed and rotated every 3 d as described earlier. On August 13, 2021, just over 5 wk after transplantation, plant height and number of leaves were recorded again for all 92 experimental plants. All aboveground plant material was removed at the soil level and placed in labeled paper bags. Roots were then removed from the soil before being washed and placed in labeled paper bags. All plant material was dried at 60 C for 72 h before being weighed.
Experiment 3: Assessing the Effects of Soil Conditioned by Vincetoxicum rossicum or Asclepias syriaca on the Success of Native Seed Mixes
We compared coverage and biomass produced by two native seed mixes in control soils and soils conditioned with 13 wk of V. rossicum or A. syriaca growth from seed. This experiment used a two by three randomized factorial “soil feedback” design (Klironomos Reference Klironomos2002; Quinn and Keough Reference Quinn and Keough2002). The factorial design tested two factors, seed mix (sun mix and semi-shade mix; Table 1) and soil treatment (V. rossicum, A. syriaca, and control). Ten replicates of each combination were conducted for a total of 60 replicates. Landscape fabric was placed in the bottom of 60 clear plastic containers (29.8 by 46.3 by 20.8 cm), each with 13 drainage holes cut in the bottom. These containers were filled with a mixture of field soil and root tissue collected from three open meadows in Toronto, ON (43.760989, −79.244806; 43.757751, −79.249238; and 43.803128, −79.183694) (15%) and pro-mix BX (85%; Premier Tech Horticulture, Rivière-du-Loup, QC, Canada). In these soil/potting media mixtures, 32 V. rossicum seeds (field-collected from Crother’s Wood, Toronto, ON, Canada 43.696812, −79.359441), 32 A. syriaca seeds (Wildflower Farm), or no seeds (control) were sown in each container. Containers were positionally randomized each week as in earlier experiments and watered as needed. After 3 wk, 20 additional V. rossicum or 7 additional A. syriaca seeds were added to each container due to poor initial germination. After 8 wk, containers were thinned to five plants (except for one container in which only four plants were left, and one in which only three plants germinated). After 13 wk, stems of all plants were removed. The roots of two randomly selected plants per container were also removed for molecular analysis of fungal associates (data not included here), while the remaining root tissue was left in the containers as a source of allelochemicals and mycorrhizae.
Two seed mixes (sun and semi-shade) used in Experiment 3
a Seed mixes were obtained from Wildflower Farm (Coldwater, ON, Canada).
b Included as a nurse crop.
Each container was then seeded with 1.0 g of one of two native seed mixes (Wildflower Farm) (Table 1) that had been allowed to soak overnight in Nutriboost 1. After 5 wk, plant community cover was measured from overhead photographs (Figure 1; Supplementary Information). Graminoids were harvested for aboveground biomass at 12 wk after seeding, and forbs (a negligible contribution to overall community cover/biomass) were harvested at 13 wk after seeding. Harvested plants were dried and weighed as described previously.
Percentage plant cover assessments for soil feedback experiment. Images were manually cropped to container edges in GIMP (A), then converted to HSV colorspace, masked at [30,25, 25] and [90, 255,255] (determined experimentally to give the best results), decomposed into channels, smoothed with a 5 x 5 gaussian convolution kernel, and binarized with Otsu’s, (Reference Otsu1979) algorithm in Python (B). From these processed images, percent cover was calculated as the ratio of non-black pixels in the image to the total number of pixels, times 100. In this example, percent cover was 45.7%.
Experiment 4: Assessing the Effects of Aqueous Extracts of Asclepias syriaca on the Growth of Vincetoxicum rossicum from Root Crowns
We compared the growth and fitness of V. rossicum plants grown from root crowns treated with extracts of A. syriaca versus water controls. For this experiment, we used a one-way independent-samples design comparing growth of treated versus untreated plants. Aqueous A. syriaca extracts were prepared based on methods by Wilson and Rice (Reference Wilson and Rice1968) and Rasmussen (Reference Rasmussen1975). Leaves of A. syriaca were collected from Whitby, ON (43.958361, −78.942260) in July and stored at −18C. Aqueous extracts were produced by adding 750 g of leaves to 2,500 ml of distilled water and homogenizing the mixture in a blender. An additional 5 L of distilled water was then added, and the suspension was boiled for 10 min and allowed to cool until safe to handle. The suspension was then filtered through household coffee filters to remove particulates, and diluted 1:1 with distilled water. From this, 100-ml aliquots were prepared and frozen until use. Extracts and distilled water controls were allowed to thaw overnight before being used to treat V. rossicum plants.
Vincetoxicum rossicum root crowns were collected from Uxbridge, ON (44.088923, −79.107089), in June 2021 and stored in freezer bags at 5 C. To set up the experiment, 46 V. rosscium root crowns were potted in Pro-Mix HP + mycorrhizae medium (Premier Tech Horticulture) using round 750-ml greenhouse pots. Root sections were weighed before planting (7.0 ± 0.1 g, mean ± SE); the mean initial biomass of roots allocated to the treatments was not significantly different (P = 0.173). Planted roots were treated with either 100 ml of A. syriaca extract (treatment, n = 23) or distilled water (control, n = 23) weekly for 5 wk. Experimental pots were positionally randomized each week and watered as needed. Five weeks after the start of the experiment, the tallest stem in each pot was measured for stem height and chlorophyll content index (CCI). Measurement of the CCI was taken as the average of three repeat measurements with a CCM-200 Plus CCI meter (Opti-Sciences, Hudson, NH, USA) from a randomly selected, recently emerged leaf (Parry et al. Reference Parry, Blonquist and Bugbee2014). Additionally, all above- and belowground plant parts were harvested and dried as described previously for biomass measurements.
Statistical Analysis
For Experiment 1, germination was compared between soil types with a one-sided Fisher’s exact test. Mean above- and belowground plant biomass were compared between soil types with a Welch’s t-test for each species. Welch’s t-tests are more reliable when the populations being compared have unequal variances.
For Experiment 2, we compared plant height immediately after transplantation and after treatment using Welch’s t-tests. Root biomass and shoot biomass were also compared between soil types using Welch’s t-tests.
For Experiment 3, the normality assumptions of ANOVA were not satisfied with either biomass or cover. For community biomass, this was addressed by converting data values to ranks that indicate relative magnitude (e.g., Quinn and Keough Reference Quinn and Keough2002). For community biomass data, this transformation allowed comparison among treatments using a two-way ANOVA and Tukey’s honest significant difference (HSD) post hoc. Percentage plant cover was compared across soil treatments and seed mixes in an exploratory analysis using a Schreirer-Ray-Hare test. We assessed assumptions of data or residual normality and heteroscedasticity using visual inspection of quantile–quantile plots and box plots respectively. Data were borderline even after rank transformation, so as a conservative measure, we used the Schreirer-Ray-Hare test designed for nonparametric data.
Because neither seed mix nor the interaction of seed mix and soil treatment were significant factors in the model, both were removed. The percentage plant cover was then compared among soil types using a Kruskal-Wallis test with Dunn’s multiple-comparison method (Dunn Reference Dunn1964; Midway et al. Reference Midway, Robertson, Flinn and Kaller2020).
For Experiment 4, we compared biomass, CCI, and stem measurements across treatments with t-tests as before. Flowering was compared between treatments using generalized linear models with a binomial logistic family.
All statistical analyses were performed in R v. 4.3.1. (R Core Team 2023) using libraries agricolae for its implementation of Tukey’s HSD test (de Mendiburu Reference de Mendiburu2021), MASS for its implementation of generalized linear models (Venables and Ripley Reference Venables and Ripley2002), and FSA (Ogle et al. Reference Ogle, Doll, Wheeler and Dinno2021) for implementation of Dunn’s (Reference Dunn1964) multiple-comparison method.
Results and Discussion
Experiment 1 compared V. rossicum and A. syriaca seedling emergence using soil from a 15-yr-old V. rossicum infestation as well as control soil from 10 m outside the infested area. The proportion of seeds germinating in invaded soil was lower for both A. syriaca (0.161) and for V. rossicum (0.033) than for either A. syriaca (0.210) or V. rossicum (0.067) in uninvaded soil, with the effect being marginal in A. syriaca (N = 720, P = 0.058) and significant in V. rossicum (N = 720, P = 0.029) (Figure 2A). Mean V. rossicum above- (N = 24, t = −0.9758(23,1), P = 0.171) and belowground biomass (N = 24, t = −0.5202(23,1), P = 0.304) were not significantly different in invaded and uninvaded soil (Figure 2B and 2C). Conversely, in A. syriaca, both aboveground biomass (14.8 ± 0.8 mg, mean ± SE) (N = 83, t = −2.0333(82,1), P = 0.023) and belowground biomass (10.4 ± 0.8mg) (N = 83, t = −3.1782(82,1), P = 0.001) were significantly lower in V. rossicum–invaded soil than in control soil (16.8 ± 0.6 mg) (Figure 2B and 2C).
Germination and early establishment of Vincetoxicum rossicum and Asclepias syriaca in V. rossicum–invaded or control (uninvaded) soil. (A) Germination, (B) aboveground biomass, and (C) belowground biomass. n.s., not statistically different (P > 0.1); *significant (P < 0.05) difference; **highly significant (P < 0.01) difference.
Soil conditioned by a mature V. rossicum infestation inhibited seed germination in V. rossicum and, to a lesser extent, A. syriaca. This is not the first time that V. rossicum has been implicated in inhibiting seed germination (Cappuccino Reference Cappuccino2004; Douglass et al. Reference Douglass, Weston and Wolfe2011). Douglass et al. (Reference Douglass, Weston and Wolfe2011) found that the presence of V. rossicum seedlings in agar resulted in reduced germination in lettuce. While these results support those of prior work, the mode of action may be entirely different. Previous experiments involved no soil, and effects on germination were likely related to allelopathy. Our observations of reduced seedling emergence in V. rossicum–infested soil could equally be related to differences in the rhizosphere. Infestations of V. rossicum are known to cause shifts in AMF communities (Bongard et al. Reference Bongard, Navaranjan, Yan and Fulthorpe2013; Dickinson et al. Reference Dickinson, Bourchier, Fulthorpe, Shen, Jones and Smith2021), and such shifts have been associated with changes in seedling emergence in other plant systems (Hartnett et al. Reference Hartnett, Samenus, Fischer and Hetrick1994; Seiwa et al. Reference Seiwa, Negishi, Eto, Hishita, Masaka and Fukasawa2020). Future work should seek to isolate the effects of common fungal associates on seed germination and seedling emergence in V. rossicum and its native competitors. In addition to inhibiting seed germination, soil from a mature V. rossicum infestation led to a reduction in above- and belowground biomass in A. syriaca seedlings. Invasive plants have previously been shown to reduce the growth of native plants by eliciting changes in soil conditions. For example, garlic mustard [Alliaria petiolata (M. Bieb.) Cavara & Grande; Cruciferae], suppresses tree seedlings in North American forests by disrupting their mutualisms with AMF (Stinson et al. Reference Stinson, Campbell, Powell, Wolfe, Callaway, Thelen, Hallett, Prati and Klironomos2006). Vincetoxicum rossicum infestations have themselves been studied in connection with changes in native plant growth (Bongard et al. Reference Bongard, Navaranjan, Yan and Fulthorpe2013; Day et al. Reference Day, Dunfield and Antunes2016; Dickinson et al. Reference Dickinson, Bourchier, Fulthorpe, Shen, Jones and Smith2021; Dukes et al. Reference Dukes, Koyama, Dunfield and Antunes2019). A study comparing 54 infested sites across southern Ontario showed that V. rossicum biomass production is enhanced by the accumulation of fungal associates, including dark septate endophytes, many of which are pathogenic (Dickinson et al. Reference Dickinson, Bourchier, Fulthorpe, Shen, Jones and Smith2021). The degree to which this enhanced biomass is related to the disruption of fungal associations in native plants is unclear; however, it is likely that changes in fungal communities play a role in V. rossicum invasions.
For Experiment 2, we compared the growth of potted A. syriaca plants transplanted into soil from a 15-yr-old V. rossicum infestation with those transplanted into soil from 10 m outside the infested area. A comparison of stem heights immediately after transplantation showed no difference in plant size between experimental groups before treatment (N = 91, t = −0.416(90,1), P = 0.678). We observed no difference in A. syriaca plant height (N = 91, t = −0.933(90,1), P = 0.177), belowground biomass (N = 91, W = 1,196, P = 0.101), or aboveground biomass (N = 91, W = 848.5, P = 0.070) between control or V. rossicum–invaded soil. Soil conditioned by a mature V. rossicum infestation had no negative impact on the growth of transplanted A. syriaca plants. Experiments 1 and 2 showed a pattern in which V. rossicum–infested soil negatively affected germination and seedling emergence of both A. syriaca and V. rossicum, but not the growth of established A. syriaca plants. These results suggest that any changes in soil conditions brought about by V. rossicum infestations, in the case of A. syriaca, are more likely to prevent the establishment of new plants than impact the fitness of existing ones. The poor performance of A. syriaca seeds casts doubt on their usefulness as a tool for site restoration. However, the effects of V. rossicum on native plants is inconsistent across species (Dukes et al. Reference Dukes, Koyama, Dunfield and Antunes2019), so we went on to test the efficacy of diverse seed mixes in V. rossicum–conditioned soil. It should be noted that the use of a single field site for soil sampling in Experiments 1 and 2 limits the scope of statistical inference that can be drawn from the results (Hurlbert Reference Hurlbert1984); however, the use of a single site also helped to control for other variations in soil conditions that might have influenced the results.
For Experiment 3, we measured mean plant cover and community biomass produced by two native seed mixes in soils conditioned by V. rossicum and A. syriaca compared with control soils. Percentage plant cover was not significantly affected by seed mix (Schreirer-Ray-Hare: n = 10, H = 0.238(1), P = 0.626) or by the interaction of seed mix and soil history (Schreirer-Ray-Hare: n = 10, H = 2.307(2), P = 0.316). Percentage plant cover was, however, significantly affected by soil history (Kruskal-Wallis test: n = 20, χ2 = 22.215, P < 0.001). Soil conditioned with A. syriaca produced significantly lower plant cover (28.760 ± 2.725%) than control soil (47.632 ± 2.266%) (z = −4.220, P < 0.001) and soil conditioned with V. rossicum (45.670 ± 3.559%) (z = −3.929, P < 0.001). Soil conditioned with V. rossicum did not differ from control soil in terms of mean plant cover (z = 0.290, P = 0.772) (Figure 3A).
(A) Community percentage cover compared among three soil treatments after 5 wk (data from both seed mixes were combined, as seed mix was not a significant factor in the model). (B) Community biomass after 13 wk of growth in soil conditioned with Asclepias syriaca, Vincetoxicum rossicum, or control (unconditioned soil). Different letters indicate significant (P < 0.05) differences among treatments.
When measuring rank-transformed community biomass in Experiment 3, we observed a significant effect of soil history (n = 10, F = 15.312(2), P < 0.001) and a significant interaction between seed mix and soil history (n = 10, F = 5.86(2), P = 0.005). When the sun seed mix was used, neither soil conditioned with V. rossicum nor soil conditioned with A. syriaca differed from control soil (P = 0.136 and P = 0.751, respectively). Soil conditioned with A. syriaca, however, produced significantly lower community biomass (13.56 ± 2.12 g) than soil conditioned with V. rossicum (26.04 ± 2.37) (n = 10, difference = −23.4, P = 0.004). When the semi-shade seed mix was used, soil conditioned with A. syriaca produced significantly lower community biomass (13.52 ± 2.12 g) than control soil (29.10 ± 1.37 g) (n = 10, difference = −31, P = 0.001), and soil conditioned with V rossicum (22.98 ± 3.03 g) (n = 10, difference = −19, P = 0.03). Community biomass was not significantly different between control soil and soil conditioned with V. rossicum (n = 10, difference = 12.0, P = 0.362) (Figure 3B).
Overall, the two native seed mixes used in Experiment 3 were not inhibited by soil conditioned by V. rossicum, but they were inhibited by soil conditioned with A. syriaca. These results indicate that reseeding has potential as a tool for restoring ecosystem function to V. rossicum–infested sites, as has proven to be the case in some other systems (Barlow et al. Reference Barlow, Mortensen and Drohan2020; Cuneo and Leishman Reference Cuneo and Leishman2015). Past research has shown the effects of V. rossicum–conditioned soil on native plant growth to be highly inconsistent (Day et al. Reference Day, Antunes and Dunfield2015, Reference Day, Dunfield and Antunes2016; Dukes et al. Reference Dukes, Koyama, Dunfield and Antunes2019). By using seed mixes containing many species, we may provide the functional redundancy required to restore ecosystem function in the face of changes in soil conditions exerted by V. rossicum. During our reseeding experiment, much of the native biomass in V. rossicum–conditioned soil was made up of grass species including A. gerardii, E. canadensis, E. hystrix, and E. virginicus, and the nurse crop L. perenne spp. multiflorum. Based on these results we advocate for the inclusion of these species in seed mixes used for post–V. rossicum restoration.
Soil conditioned with A. syriaca yielded significantly lower percentage cover and biomass of native plants. The allelopathic effects of A. syriaca have been reported previously in Europe, where the plant is an important introduced weed in agricultural systems (Cramer and Burnside Reference Cramer and Burnside1982; Follak et al. Reference Follak, Bakacsy, Essl, Hochfellner, Lapin, Schwarz, Tokarska-Guzik and Wołkowycki2021; Nádasy et al. Reference Nádasy, Pásztor, Béres and Szilágyi2018; Rasmussen Reference Rasmussen1975). Asclepias syriaca would seem a logical choice as a species for ecological restoration after V. rossicum infestation, not only because of its relatedness, but because its displacement is problematic for monarch butterflies for which V. rossicum is an oviposition sink (Casagrande and Dacey Reference Casagrande and Dacey2007). Our results, however, highlight the possible allelopathic effects of A. syriaca and caution against its use. For this reseeding experiment, we chose to inoculate experimental soils by growing the test plants in greenhouse conditions for a period of 13 wk, rather than collecting soils from infested habitats. This choice allowed us to compare the effects V. rossicum and A. syriaca more accurately, because field sites with a monoculture of A. syriaca could not be found. Additionally, allelopathic compounds produced by V. rossicum have been found to be relatively unstable, persisting only for short periods in collected soil (Gibson et al. Reference Gibson, Vaughan and Milbrath2015). Although 13 wk has been shown to be enough time for plant roots to recruit fungal associates and produce bioactive concentrations of allelopathic chemicals (Day et al. Reference Day, Antunes and Dunfield2015; Dukes et al. Reference Dukes, Koyama, Dunfield and Antunes2019, Weißhuhn and Prati Reference Weißhuhn and Prati2009), future research should focus on testing seed mixes in soil collected from mature infestations and under field conditions.
Building on the effects of A. syriaca on native seed mixes, we tested the effects of aqueous A. syriaca extracts on V. rossicum plants grown from rootstock (Experiment 4) to determine whether phytotoxins found in A. syriaca tissue might be useful as a tool to suppress V. rossicum regrowth after removal. Stem height was significantly higher in V. rossicum plants treated with A. syriaca extracts (29.1 ± 2.0 cm) than in controls (15.0 ± 0.9 cm) (N = 46, W = 489.5, P < 0.001) (Figure 4A). Aboveground biomass was significantly higher in plants treated with A. syriaca extracts (0.433 ± 0.030 mg) than in controls (0.260 ± 0.027 mg) (N = 46, t = 4.7334(45,1), P < 0.001) (Figure 4B). CCI was also significantly higher in plants treated with A. syriaca extracts (19.7 ± 0.6) than in controls (15.8 ± 0.6) (N = 46, t = 4.3511(45,1), P < 0.001) (Figure 4C). In addition to exhibiting increased growth, plants treated with aqueous extracts of A. syriaca were significantly more likely to produce flowers (78%) than control plants (4.3%) (N = 46, z = −3.833(45,1), P < 0.001).
Growth of Vincetoxicum rossicum plants grown from rootstock and treated with aqueous extracts prepared from Asclepias syriaca leaves (white) or distilled water (gray). (A) Stem height, (B) aboveground biomass, and (C) chlorophyll content index (CCI). Asterisks indicate highly significant differences (***P < 0.0001; *P < 0.01) in group means.
The positive effects of A. syriaca extracts on V. rossicum plant growth and fitness were surprising, but not without precedent. Cramer and Burnside (Reference Cramer and Burnside1982) found that decomposing A. syriaca tissue enhanced growth in Sorghum bicolor L. (Poaceae), even though aqueous extracts of A. syriaca are phytotoxic to the same species (Rasmussen Reference Rasmussen1975). In our experiment, the cause of the increased V. rossicum growth is not clear, but may be the result of a fertilization effect if A. syriaca extracts contained previously limiting nutrients. Several studies have also demonstrated hormesis in response to low doses of allelopathic chemicals (Abbas et al. Reference Abbas, Nadeem, Tanveer and Chauhan2017). For example, Santa-Maria feverfew (Parthenium hysterophorus L.; Asteraceae) is known to produce several phytochemicals, including the sesquiterpene lactone parthenin. Although parthenin is known to inhibit the growth of neighboring plants, some species, including wild mustard (Sinapis arvensis L.; Brassicaceae), show enhanced growth when exposed to low doses (Belz Reference Belz2008). In the future, dose–response studies should be conducted to accurately characterize the relationship between V. rossicum and phytochemicals produced by A. syriaca (Belz et al. Reference Belz, Velini, Duke, Fujii and Hiradate2007).
The cause of earlier flowering in extract-treated plants is also unclear. Early flowering could be a response to stress (e.g., Takeno Reference Takeno2016), or it could simply be an indication of increased reproductive output in treated plants (Cappuccino Reference Cappuccino2004). Regardless of the specific mechanism, these results provide further evidence that A. syriaca may not be a suitable species for use in the restoration of ecosystems invaded by V. rossicum.
Our findings contribute to an understanding of the ecology of V. rossicum–invaded sites and can direct restoration efforts in two key ways. First, V. rossicum–conditioned soil negatively affected V. rossicum seedling establishment, suggesting that residual V. rossicum seeds will not necessarily dominate the competition after the removal of mature plants from an infested site. Second, the use of diverse native seed mixes shows great potential for restoring productivity and function to ecosystems affected by V. rossicum infestations. Testing the use of similar seed mixes at sites that have been cleared of V. rossicum infestations across a range of soil conditions could help to identify the native species best suited for restoration on a site-by-site basis.
Supplementary material
To view supplementary material for this article, please visit https://doi.org/10.1017/inp.2024.7
Data availability statement
Data generated or analyzed during this study are available from the corresponding author upon reasonable request.
Acknowledgments
The authors wish to thank the following for their assistance: Jenna Wolno, Alexandra Rossi, Sophie Cation, Ayumi Akimoto, Gary Taylor, Thibault Bardy-Renard, and Julian Alvarez-Barkham. Thanks also to Harris Snyder for his feedback on the image processing methodology, to Marie Thieburg for identifying field sites, to Less Mess for donating triple mix in the transplant experiment, and to CredoSense for donating temperature and relative humidity loggers for the greenhouse. This project was funded by the Toronto and Region Conservation Authority through the MITACS Accelerate Program, Natural Sciences and Engineering Research Council of Canada (NSERC), Agriculture and Agri-Food Canada, the Invasive Species Centre, the Ontario Ministry of Natural Resources and Forestry, and the Mitacs Elevate fellowship program in partnership with Ducks Unlimited Canada. No competing interests have been declared.
Open access
