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Exploiting phenotypic and genotypic diversity against Colletotrichum truncatum in chilli hybrids developed using resistant breeding lines

Published online by Cambridge University Press:  01 February 2024

H.M.S.N. Herath Open the ORCID record for H.M.S.N. Herath [Opens in a new window] ,
M. Y. Rafii ,
Siti Izera Ismail ,
Juju Nakasha Jaafar  and
Shairul Izan Ramlee
H.M.S.N. Herath
Affiliation:
Field Crops Research and Development Institute, Mahailluppallama, Sri Lanka
M. Y. Rafii
Affiliation:
Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang, Malaysia
Siti Izera Ismail
Affiliation:
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
Juju Nakasha Jaafar
Affiliation:
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
Shairul Izan Ramlee*
Affiliation:
Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang, Malaysia Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
*
Corresponding author: Shairul Izan Ramlee; Email: shairul@upm.edu.my
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Abstract

In an effort to control anthracnose disease, one of the major problems that has been faced by farmers, 14 chilli hybrids and their parents were screened phenotypically using the fruit inoculation method under laboratory conditions. Genotypic screening of 14 chilli hybrids and their parents was done by the identified polymorphic markers, HpmsE 051 and HpmsE 082. Based on the phenotypic and genotypic data, chilli hybrids, H1, H2, H3, H4, H6, H7, H8, H9, H11 and H12 were identified as resistant chilli hybrids against anthracnose disease caused by the C. truncatum. Molecular markers, HpmsE 051 and HpmsE 082 could be utilized as polymorphic markers to isolate resistant genotypes against C. truncatum.

Keywords

disease resistancefruit inoculationhybridsparentspolymorphic markers

Information

Type
Research Article
Information
Plant Genetic Resources , Volume 22 , Issue 1 , February 2024 , pp. 37 - 44
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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Footnotes

This article has been updated since its original publication. A notice detailing this change can be found here: https://doi.org/10.1017/S147926212400011X

References

Ananthan, R, Subhash, K and Longvah, T (2018) Capsaicinoids, amino acid and fatty acid profiles in different fruit components of the world hottest Naga king chilli (Capsicum chinense Jacq). Food Chemistry 238, 51–57.CrossRefGoogle ScholarPubMed
Anilkumar, C (2021) Breeding potential of crosses derived from parents differing in fruiting habit traits in chilli (Capsicum annuum L.). Genetic Resources and Crop Evolution 68, 45–50.CrossRefGoogle Scholar
Chunying, S, Sheng, M, Zheng, Z, Alain, P and Li, W (2015) Scientia Horticulturae Resistances to anthracnose (Colletotrichum acutatum) of Capsicum mature green and ripe fruit are controlled by a major dominant cluster of QTLs on chromosome P5. Scientia Horticulturae 181, 81–88.Google Scholar
Doyle, J and Doyle, J (1987) DNA isolation from small amounts of plant tissue. Phytochemical Bulleting 19, 11–15.Google Scholar
FAO STAT (2021) https://www.fao.org/faostat/en/#data/QCL. Retrived on 17.09.2023.Google Scholar
Garg, R, Kumar, S, Kumar, R, Loganathan, M, Saha, S, Kumar, S, Rai, AB and Roy, BK (2013) Novel source of resistance and differential reactions on chilli fruit infected by Colletotrichum capsici. Australasian Plant Pathology 42, 227–233.CrossRefGoogle Scholar
Herath, HMSN, Rafii, MY, Smil, SI, Jaafar, JN and Ramlee, SI (2022) Genetic diversity of inbred lines in chilli based on phenotypic and genotypic responses against Colletotrichum truncatum. Archives of Phytopathology and Plant Protection 55, 583–596.CrossRefGoogle Scholar
Kim, SH, Yoon, JB, Do, JW and Park, HG (2008) A major recessive gene associated with anthracnose resistance to Colletotrichum capsici in chili pepper (Capsicum annuum L.). Breeding Science 58, 137–141.CrossRefGoogle Scholar
Kothari, SL, Joshi, A, Kachhwaha, S and Ochoa-Alejo, N (2010) Chilli peppers - A review on tissue culture and transgenesis. Biotechnology Advances 28, 35–48.CrossRefGoogle ScholarPubMed
Lee, J, Do, JW and Yoon, JB (2011). Development of STS markers linked to the major QTLs for resistance to the pepper anthracnose caused by Colletotrichum acutatum and C. capsici. Horticulture Environment and Biotechnology 52, 596–601.CrossRefGoogle Scholar
Ly, VA, Truong, TPT and Nguyen, TH (2020) Application of anthracnose resistance-associated molecular markers in the detection of resistant chili pepper cultivars in Vietnam. Science &TechnologyDevelopment Journal 23, 576–584.Google Scholar
MARDI (1997) Panduan Pengeluaran Sayur-sayuran. Kuala Lumpur, Malaysia: Institut Penyelidikan dan Kemajuan Pertanian (MARDI), pp. 32–47.Google Scholar
Mashuk, P, Khumpeng, N, Wasee, S, Taylor, PWJ and Mongkolporn, O (2009) Inheritance of resistance to anthracnose (Colletotrichum capsici) at seedling and fruiting stages in chili pepper (Capsicum spp.). Plant Breeding 128, 701–706.CrossRefGoogle Scholar
Meena, OP, Dhaliwal, MS and Jindal, SK (2020) Heterosis breeding in chilli pepper by using cytoplasmic male sterile lines for high-yield production with special reference to seed and bioactive compound content under temperature stress regimes. Scientia Horticulturae 262, 109036.CrossRefGoogle Scholar
Mishra, R, Rout, E and Joshi, RK (2019a) Identification of resistant sources against anthracnose disease caused by Colletotrichum truncatum and Colletotrichum gloeosporioides in Capsicum annuum L. Proceedings of the National Academy of Sciences India Section B - Biological Sciences 89, 517–524.CrossRefGoogle Scholar
Mishra, R, Rout, E, Mohanty, JN and Joshi, RK (2019b) Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.). 3 Biotech 9, 1–13.CrossRefGoogle ScholarPubMed
Montri, P, Pathom, N and Ten, C (2009) Pathotypes of Colletotrichum capsici, the causal agent of chili anthracnose, in Thailand. Plant Disease 93, 17–20.CrossRefGoogle ScholarPubMed
Nanda, C, Mohan Rao, A, Ramesh, S, Hittalmani, S and Prathibha, VH (2016) Tagging SSR markers associated with genomic regions controlling anthracnose resistance in chilli (Capsicum baccatum L.). Vegetos (Bareilly, India) 29, 130–134.CrossRefGoogle Scholar
Noor, NM and Zakaria, L (2018) Identification and characterization of Colletotrichum spp. associated with chilli anthracnose in peninsular Malaysia. European Journal of Plant Pathology 151, 961–973.CrossRefGoogle Scholar
Park, HK, Kim, BS and Lee, WS (1990) Inheritance of resistance to anthracnose (Colletotrichum spp.) in pepper (Capsicum annuum L.) II. Genetic analysis of resistance to Colletotrichum dematium. Korean Journal of Horticultural Science and Technology 31, 207–212.Google Scholar
Ridzuan, R (2018). Development of anthracnose resistant chilli varieties through marker assisted pedigree selection (PhD thesis). Retrieved from http://ethesis.upm.edu.my/.Google Scholar
Sahid, ZD, Syukur, M and Maharijaya, A (2020) Combining ability and heterotic effects of chili pepper (Capsicum annuum l.) genotypes for yield components and capsaicin content. Sabrao Journal of Breeding and Genetics 52, 390–401.Google Scholar
Shuro, AR (2017) Review paper on approaches in developing inbred lines in cross-pollinated crops. Biochemistry and Molecular Biology 2, 40.CrossRefGoogle Scholar
Siddappa, S, Ravindra, M and Shashikanth, E (2019) Combining ability analysis in Chilli (Capsicum Annum L.). Agricultural Science Digest 39, 220–223.Google Scholar
Silva, DD, Groenewald, JZ, Crous, PW, Ades, PK, Nasruddin, A, Mongkolporn, O and Taylor, PWJ (2019) Identification, prevalence and pathogenicity of Colletotrichum species causing anthracnose of Capsicum annuum in Asia. IMA Fungus 10, 1–32.CrossRefGoogle ScholarPubMed
Sindhusha, P and Rawat, M (2020). Genetic variability and inter-relationship studies among growth, yield and quality parameters in Chilli (Capsicum annuum L.). Journal of Pharmacognosy and Phytochemistry 9, 1526–1530.CrossRefGoogle Scholar
Son, S, Kim, S, Lee, KS, Oh, J, Choi, I, Do, JW and Yoon, JB (2021) Identification of the Capsicum baccatum NLR protein CbAR9 conferring disease resistance to anthracnose. International Journal of Molecular Sciences 22, 12612.CrossRefGoogle ScholarPubMed
Srivastava, A and Mangal, M (2019) Capsicum Breeding: History and Development. In Ramchiary N and Kole C (ed.), The Capsicum Genome. Switzerland: Springer Nature, pp. 25–56.CrossRefGoogle Scholar
Suwor, P, Sanitchon, J, Thummabenjapone, P, Kumar, S and Techawongstien, S (2017) Inheritance analysis of anthracnose resistance and marker-assisted selection in introgression populations of chilli (Capsicum annuum L.). Scientia Horticulturae 220, 20–26.CrossRefGoogle Scholar
Thakur, H, Jindal, SK, Sharma, A and Dhaliwal, MS (2019) A monogenic dominant resistance for leaf curl virus disease in chilli pepper (Capsicum annuum L.). Crop Protection 116, 115–120.CrossRefGoogle Scholar
Voorrips, RE, Finkers, R and Sanjaya, L (2004) QTL mapping of anthracnose (Colletotrichum spp.) resistance in a cross between Capsicum annuum and C. chinense. Theoretical and Applied Genetics 109, 1275–1282.CrossRefGoogle Scholar
Welideniya, WA, Rienzie, KDRC, Wickramaarachchi, WART and Aruggoda, AG B (2019) Characterization of fungal pathogens causing anthracnose in capsicum pepper (Capsicum annuum L.) and their seed-borne nature. Ceylon Journal of Science 48, 261.CrossRefGoogle Scholar
Yi, G, Lee, J, Lee, S, Choi, D and Kim, B (2006) The exploitation of pepper EST – SSRs and an SSR-based linkage map. Theory and Applied Genetics (2006), 114, 113–130.CrossRefGoogle Scholar
Ying, SC, Li, MS, Hai, ZZ, Alain, P, Hao, WL and Xi, ZB (2015) Resistances to anthracnose (Colletotrichum acutatum) of Capsicum mature green and ripe fruit are controlled by a major dominant cluster of QTLs on chromosome P5. Scientia Horticulturae 181, 81–88.Google Scholar
Zhao, Y, Liu, Y, Zhang, Z, Cao, Y, Yu, H, Ma, W, Zhang, B, Wang, R, Gao, J and Wang, L (2020) Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense “PBC932.”. BMC Plant Biology 20, 1–8.CrossRefGoogle ScholarPubMed