Hostname: page-component-89b8bd64d-4ws75 Total loading time: 0 Render date: 2026-05-10T19:56:38.288Z Has data issue: false hasContentIssue false
  • English
  • Français

The association of enterotoxigenic and enteropathogenicEscherichia coli and other enteric pathogens with childhood diarrhoea in Yugoslavia

Published online by Cambridge University Press:  15 May 2009

M. Čobeljić ,
D. Mel ,
B. Arsić ,
Lj. Krstić ,
B. Sokolovski ,
B. Nikolovski ,
E. Šopovski ,
M. Kulauzov  and
S. Kalenić
M. Čobeljić
Affiliation:
Institute of Preventive Medicine, Military Medical Academy, 11000 Belgrade, Yugoslavia
D. Mel
Affiliation:
Institute of Preventive Medicine, Military Medical Academy, 11000 Belgrade, Yugoslavia
B. Arsić
Affiliation:
Institute of Preventive Medicine, Military Medical Academy, 11000 Belgrade, Yugoslavia
Lj. Krstić
Affiliation:
Institute of Preventive Medicine, Military Medical Academy, 11000 Belgrade, Yugoslavia
B. Sokolovski
Affiliation:
Institute of Preventive Medicine, 91000 Skopje, Yugoslavia
B. Nikolovski
Affiliation:
Institute of Preventive Medicine, 91000 Skopje, Yugoslavia
E. Šopovski
Affiliation:
Institute of Preventive Medicine, 91000 Skopje, Yugoslavia
M. Kulauzov
Affiliation:
Institute of Public Health, 21000 Novi Sad, Yugoslavia
S. Kalenić
Affiliation:
Institute of Public Health, 41000 Zagreb, Yugoslavia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the 'Save PDF' action button.

The presence of enterotoxigenic and enteropathogenicEscherichia coli (ETEC and EPEC, respectively) was investigated in stool specimens of 1082 preschool children with diarrhoea and in stools of 335 healthy controls in localities in southern Yugoslavia, as well as in 566 children with diarrhoea and in 231 controls living in northern part of the country, during the seasonal peak (August— November) of enteric diseases in 1986.

ETEC were found in 114 (10·5%) children with diarrhoea and in 14 (4·2%) controls (P < 0·001) in the southern part, and in 26 (4·6%) ill children and one (0·4%) well child (P < 0·005) in the northern part of Yugoslavia. EPEC were isolated from stools of 85 (7·9%) children with diarrhoea and of 14 (4·2%) well children (P < 0·05) in localities of southern Yugoslavia, and from 22 (3·9%) ill children and from 10 (4·3%) controls in northern Yugoslavia. Nineteen EPEC strains expressed localized adherence to HEp-2 tissue culture cells; all were isolated from stools of ill children.

In southern Yugoslavia, where other enteropathogens were sought, the most commonly found agents in ill children were shigellae (17·5%), rotavirus (11·8%), ETEC, and EPEC. Potential pathogens were detected in 44·5 % cases of sporadic diarrhoea and in 15·8% controls.

This study revealed that ETEC were associated with acute diarrhoeal disease in Yugoslav preschool children. On the other hand, the diagnosis of EPEC-diarrhoea by routine determination of serogroup established the association of these agents with sporadic diarrhoea only in the 0-2 years age categories in all investigated localities. In the less developed southern part of Yugoslavia bacteria were the predominant causative agents of enteric illness during the seasonal peak of this disease.

Information

Type
Special Article
Information
Epidemiology & Infection , Volume 103 , Issue 1 , August 1989 , pp. 53 - 62
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

Black, R. E., Merson, M. H., Rahman, M. M., Yunus, M., Alim, A., Huq, I., Yolken, R. H. & Curlin, G. T. (1980). Two-year study of bacterial, viral, and parasitic agents associated with diarrhea in rural Bangladesh. Journal of Infectious Diseases 142, 660–664.CrossRefGoogle ScholarPubMed
Brunton, J., Hinde, D., Langston, C., Gross, R., Rowe, B. & Gurwith, M. (1980). Enterotoxigenic Escherichia coli in Central Canada. Journal of Clinical Microbiology 11, 343–348.CrossRefGoogle ScholarPubMed
Chatkaeomorakot, A., Echeverria, P., Taylor, D. N., Bettelheim, K. A., Blacklow, N. R., Sethabutr, O., Seriwatana, J. & Kaper, J. (1987). HeLa cell-adherent Escherichia coli in children with diarrhea in Thailand. Journal of Infectious Diseases 156, 669–672.CrossRefGoogle ScholarPubMed
Dean, A. G., Ching, Y. C., Williams, R. G. & Harden, L. B. (1972). Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. Journal of Infectious Diseases 125, 407–410.CrossRefGoogle Scholar
De Mol, P., Brasseur, D., Hemelhof, W., Kalala, T., Butzler, J. P. & Vis, H. L. (1983). Enteropathogenic agents in children with diarrhoea in rural Zaire. Lancet 1, 516–518.CrossRefGoogle ScholarPubMed
Donta, S. T., Wallace, R. B., Whipp, S. C. & Olarte, J. (1977). Enterotoxigenic Escherichia coli and diarrheal disease in Mexican children. Journal of Infectious Diseases 135, 482–485.Google Scholar
Echeverria, P., Seriwatana, J., Taylor, D. N., Yanggratoke, S. & Tirapat, C. (1985). A comparative study of enterotoxigenic Escherichia coli, Shigella, Aeromonas, and Vibrio as etiologies of diarrhea in northeastern Thailand. American Journal of Tropical Medicine and Hygiene 34, 547–554.CrossRefGoogle ScholarPubMed
Echeverria, P., Taylor, D. N., Donohue-Rolfe, A., Supawat, K., Ratchtrachenchai, O., Kaper, J. & Keusch, G. T. (1987). HeLa cell adherence and cytotoxin production by enteropathogenic Escherichia coli isolated from infants with diarrhea in Thailand. Journal of Clinical Microbiology 25, 1519–1523.CrossRefGoogle ScholarPubMed
Edwards, P. R. & Ewing, W. H. (1972). Identification of Enterobacteriaceae, 3rd edn.Burgess Publishing Company.Google Scholar
Evans, D. J., Evans, D. G. & DuPont, H. L. (1979). Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea-pig erythrocytes in the presence and absence of mannose. Infection and Immunity 23, 336–346.CrossRefGoogle ScholarPubMed
Gross, R. J. & Rowe, B. (1985). Escherichia coli diarrhoea. Journal of Hygiene 95, 531–550.CrossRefGoogle ScholarPubMed
Guerrant, R. L., Brunton, L. L., Schaitman, T. C., Rebhun, L. L. & Gilman, A. G. (1974). Cyclic adenosine monophosphate and alteration of chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infection and Immunity 10, 320–327.CrossRefGoogle ScholarPubMed
Guerrant, R. L., Kirchhoff, L. V., Shields, D. S., Nations, M. K., Leslie, J., Sousa, M. A., Araujo, J. G., Correia, L. L., Saver, K. T., McClelland, K. E., Trowbridge, F. L. & Hughes, J. M. (1983). Prospective study of diarrheal illnesses in Northeastern Brazil: patterns of disease, nutritional impact, etiologies, and risk factors. Journal of Infectious Diseases 148, 986–997.Google Scholar
Hughes, J. M., Rouse, J. D., Barada, F. A. & Guerrant, R. L. (1980). Etiology of summer diarrhea among the Navajo. American Journal of Tropical Medicine and Hygiene 29, 613–619.CrossRefGoogle ScholarPubMed
Kapikian, A. Z., Kim, H. W., Wyatt, R. G., Lee Cline, W., Arrobio, J. O., Brandt, C. D., Rodriguez, W. J., Sack, D. A., Chanock, R. M. & Parrot, R. H. (1976). Human reovirus-like agent as the major pathogen associated with ‘winter’ gastroenteritis in hospitalized infants and young children. New England Journal of Medicine 294, 965–972.Google Scholar
Karch, H., Heesemann, J. & Laufs, R. (1987). Phage associated cytotoxin production by and enteroadhesiveness of enteropathogenic Escherichia coli isolated from infants with diarrhea in West Germany. Journal of Infectious Diseases 155, 708–715.CrossRefGoogle ScholarPubMed
Konowalchuk, J., Speirs, J. I. & Stavric, S. (1977). Vero response to a cytotoxin of Escherichia coli. Infection and Immunity 18, 775–779.Google Scholar
Koopman, J. S., Turkish, V. J., Monto, A. S., Gouvea, V., Srivastava, S. & Isaacson, R. E. (1984). Patterns and etiology of diarrhea in three clinical settings. American Journal of Epidemiology 119, 114—123.CrossRefGoogle ScholarPubMed
Leksomboon, U., Echeverria, P., Suvongse, C. & Duangmani, C. (1981). Viruses and bacteria in pediatric diarrhea in Thailand: a study of multiple antibiotic-resistant enteric pathogens. American Journal of Tropical Medicine and Hygiene 30, 1281–1290.CrossRefGoogle ScholarPubMed
Levine, M. M. (1987). Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. Journal of Infectious Diseases 155, 377–389.CrossRefGoogle ScholarPubMed
Mathewson, J. J., Johnson, P. C., Dupont, H. L., Satterwite, T. K. & Winsor, D. K. (1986). Pathogenicity of enteroadherent Escherichia coli in adult volunteers. Journal of Infectious Diseases 154, 524–527.Google Scholar
Mathewson, J. J., Oberhelman, R. A., DuPont, H. L., Cabada, F. J. & Garibay, E. V. (1987). Enteroadherent Escherichia coli as a cause of diarrhea among children in Mexico. Journal of Clinical Microbiology 25, 1917–1919CrossRefGoogle ScholarPubMed
Nataro, J. P., Scaletsky, I. C. A., Kaper, J. B., Levine, M. M. & Trabulsi, L. R. (1985a). Plasmid-mediated factors conferring diffuse and localized adherence of entaropathogenic Escherichia coli. Infection and Immunity 48, 378–383.Google Scholar
Nataro, J. P., Baldini, M. M., Kaper, J. B., Black, R. E., Bravo, N. & Levine, M. M. (1985b). Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. Journal of Infectious Diseases 152, 560–565.CrossRefGoogle ScholarPubMed
Nataro, J. P., Kaper, J. B., Robins-Browne, R., Prado, V., Vial, P. & Levine, M. M. (1987). Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatric Infectious Diseases Journal 6, 829–831.Google Scholar
Robins-Browne, R. M. (1987). Traditional enteropathogenic Escherichia coli of infantile diarrhea. Journal of Infectious Diseases 9, 28–53.Google ScholarPubMed
Scaletsky, I. C. A., Silva, M. L. M. & Trabulsi, L. R. (1984). Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infection and Immunity 45, 534–536.CrossRefGoogle ScholarPubMed
Spencer, H. C., Wells, J. G., Gary, W. G., Sondy, J., Puhr, N. D. & Feldman, R. A. (1980). Diarrhea in a non-hospitalized rural Salvadoran population: the role of enterotoxigenie Escherichia coli and rotaviruses. American Journal of Tropical Medicine and Hygiene 29, 246–253.Google Scholar
Stintzing, G., Bäck, E., Tufveson, B., Johnson, T., Wadström, T. & Habte, D. (1981). Seasonal fluctuations in the occurrence of enterotoxigenic bacteria and rotavirus in paediatric diarrhoea in Addis Ababa. Bulletin of the World Health Organization. 59, 67–73.Google ScholarPubMed
Who, Geneva (1983). Weekly Epidemiological Report 58, 241.Google Scholar
Who, Copenhagen (1981). Surveillance and control of acute diarrhoeal diseases. Report on a WHO meeting. Rome, 8–11 04 1980. EURO Reports and Studies 44, pp. 2–8.Google Scholar