Hostname: page-component-89b8bd64d-ktprf Total loading time: 0 Render date: 2026-05-11T21:53:18.388Z Has data issue: false hasContentIssue false
  • English
  • Français

Novel sequence types (STs) of Staphylococcus aureus isolates causing clinical and subclinical mastitis in flocks of sheep in the northeast of Brazil

Published online by Cambridge University Press:  21 July 2011

Lara M de Almeida ,
Mayra Zilta PRB de Almeida ,
Carla L de Mendonça  and
Elsa M Mamizuka
Lara M de Almeida*
Affiliation:
Department of Clinical Analysis, Pharmaceutical Science Faculty, University of São Paulo, SP, Brazil
Mayra Zilta PRB de Almeida
Affiliation:
Clinic of Ruminants, Federal University of Pernambuco, PE, Brazil
Carla L de Mendonça
Affiliation:
Clinic of Ruminants, Federal University of Pernambuco, PE, Brazil
Elsa M Mamizuka
Affiliation:
Department of Clinical Analysis, Pharmaceutical Science Faculty, University of São Paulo, SP, Brazil
*
*For correspondence; e-mail: larameal@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Staphylococcus aureus is one of the most important infectious mastitis causative agents in small ruminants. In order to know the distribution of Staph. aureus strains associated with infectious mastitis in flocks of sheep in the northeast of Brazil and establish whether these clones are related to the strains distributed internationally, this study analysed the genetic diversity of Staph. aureus isolates from cases of clinical and subclinical mastitis in ewes by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). In this research, 135 ewes with mastitis from 31 sheep flocks distributed in 15 districts were examined. Staph. aureus was isolated from sheep milk in 9 (29%) out of 31 herds located in 47% of the districts surveyed. MLST analysis allowed the identification of four STs (ST750, ST1728, ST1729 and ST1730). The last three with their respective novel alleles (glp-220; pta-182 and yqil-180) were recently reported in the Staph. aureus MLST database (http://www.mlst.net). Each novel allele showed only a nucleotide different from those already described. The occurrence of CC133 (ST750 and ST1729) in this study is in agreement with other reports that only a few clones of Staph. aureus seem to be responsible for most cases of mastitis in dairy farms and that some of these clones may have broad geographic distribution. However, the prevalence of CC5 (ST1728 and ST1730)—an important group related to cases of colonization or infection in humans—differs from previous studies by its widespread occurrence and may suggest human contamination followed by selective pressures of the allelic diversifications presented for these STs.

Keywords

Staphylococcus aureusmastitissheep flockmultilocus sequence typing

Information

Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 3 , August 2011 , pp. 373 - 378
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Aires-de-Sousa, M, Parente, CESR, Vieira-da-Motta, O, Bonna, ICF, Silva, DA & Lencastre, H 2007 Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine and caprine milk samples collected in Rio de Janeiro state, Brazil. Applied and Environmental Microbiology 73 3845–3849CrossRefGoogle Scholar
Annemuller, C, Lammler, C & Zschock, M 1999 Genotyping of Staphylococcus aureus isolated from bovine mastitis. Veterinary Microbiology 69 217–224CrossRefGoogle ScholarPubMed
Barrett, TJ, Gerner-Smidt, P & Swaminathan, B 2006 Interpretation of pulsed-field gel electrophoresis patterns in foodborne disease investigations and surveillance. Foodborne Pathogens and Disease 3 20–31CrossRefGoogle ScholarPubMed
Bergonier, D, de Cremoux, R, Rupp, R, Lagriffoul, G & Berthelot, X 2003 Mastitis of dairy small ruminants. Veterinary Research 34 689–716CrossRefGoogle ScholarPubMed
Clinical and Laboratory Standards Institute (CLSI) 2007 Performance standards for antimicrobial susceptibility testing: 17th Informational supplement. M100-S17, vol. 27, n1. Wayne PA, USA: Clinical and Laboratory Standards InstituteGoogle Scholar
Diffay, BC, Mc Kenzie, D, Wolf, C & Pugh, DG 2005 [Approach and examination of sheep and goats] In: [Clinic of Sheep and Goats] (Ed. Pugh, DG) pp 1–19. São Paulo, Brazil: RocaGoogle Scholar
Enright, MC, Day, NPJ, Davies, CE, Peacock, SJ & Spratt, BG 2000 Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. Journal of Clinical Microbiology 38 1008–1015CrossRefGoogle ScholarPubMed
Enright, MC, Robinson, DA, Randle, G, Feil, EJ, Grundmann, H & Spratt, BG 2002 The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proceedings of the National Academy of Sciences USA 99 7687–7692CrossRefGoogle ScholarPubMed
Feil, EJ, Li, BC, Aanensen, DM, Hanage, WP & Spratt, BG 2004. eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. Journal of Bacteriology 186 1518–1530CrossRefGoogle ScholarPubMed
Fitzgerald, JR, Meaney, WJ, Hartigan, PJ, Smyth, CJ & Kapur, V 1997 Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows. Epidemiology and Infection 119 261–269CrossRefGoogle ScholarPubMed
Fitzgerald, JR; Sturdevant, DE, Mackie, SM, Oil, SR & Musser, JM 2001 Evolutionary genomics of Staphylococcus aureus: insights into the origin of methicillin-resistant strains and the toxic shock syndrome epidemic. Proceedings of the National Academy of Sciences USA 98 8821–8826CrossRefGoogle ScholarPubMed
Kapur, V, Sischo, WM, Greer, RS, Whittman, TS & Musser, JM 1995 Molecular population genetic analysis of Staphylococcus aureus recovered from cows. Journal of Clinical Microbiology 33 376–380CrossRefGoogle ScholarPubMed
Katsuda, K, Hata, E, Kobayashi, H, Kohmoto, M, Kawashima, K, Tsunemitsu, H & Eguchi, M 2005. Molecular typing of Staphylococcus aureus isolated from bovine mastitic milk on the basis of toxin genes and coagulase gene polymorphisms. Veterinary Microbiology 25 301–305CrossRefGoogle Scholar
Kwon, NH, Park, KT, Moon, JS, Jung, WK, Kim, SH, Kim, JM, Hong, SK, Koo, HC, Joo, YS & Park, YO 2005 Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. Journal of Antimicrobial Chemotherapy 56 624–632CrossRefGoogle ScholarPubMed
Lee, JH 2003 Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Applied and Environmental Microbiology 69 6489–6494CrossRefGoogle ScholarPubMed
Lindsay, JA & Holden, MT 2006 Understanding the rise of the superbug: investigation of the evolution and genomic variation of Staphylococcus aureus. Functional and Integrative Genomics 6 186–201CrossRefGoogle ScholarPubMed
Menzies, PI & Ramanoon, SZ 2001 Mastitis of sheep and goats. Veterinary Clinics of North America: Food Animal Practice 17 333–355Google ScholarPubMed
Mørk, T, Tollersrud, T, Kvitle, B, Jorgensen, HJ & Waage, S 2005 Comparison of Staphylococcus aureus genotypes recovered from cases of bovine, ovine and caprine mastitis. Journal of Clinical Microbiology 43 3979–3984CrossRefGoogle ScholarPubMed
National Mastitis Council 1999 Laboratory Handbook on Bovine Mastitis. Madison WI, USA: National Mastitis CouncilGoogle Scholar
Pfaller, MA 1993 Chromosomal restriction fragment analisys by pulsed-field gel electrophoresis. In: Clinical Microbiology Procedures Handbook, (Ed.Isemberg, HD) Washington DC, USA: American Society for MicrobiologyGoogle Scholar
Quinn, PJ, Carter, ME, Markey, B & Carter, GR 1994 Clinical Veterinary Microbiology. London, UK: Wolfe Publishing. p. 648Google Scholar
Rabello, RF, Moreira, BM, Lopes, RMM, Teixeira, LM, Riley, LW & Castro, ACD 2007 Multilocus sequence typing of Staphylococcus aureus isolates recovered from cows with mastitis in Brazilian dairy herds. Journal of Medical Microbiology 56 1505–1511CrossRefGoogle ScholarPubMed
Radostits, OM, Gay, CC, Blood, DC & Hinchcliff, KW 2000 Veterinary Medicine 9th Edition. London, UK: W.B. Saunders. p. 1877Google Scholar
Sambrook, J, Fritsch, EF & Maniatis, T 1989 Molecular Cloning: a Laboratory Manual 2nd Edition, v.3, Appendix B, Cold Spring Harbor Laboratory Press, USAGoogle Scholar
Schalm, OW, Carroll, EJ & Jain, NC 1971 Bovine Mastitis. Philadelphi PA, USA: Lea&Febiger. p. 360Google Scholar
Smith, EM, Green, LE, Medley, GF, Bird, HE, Fox, LK, Schukken, YH, Kruze, JV, Bradley, AJ, Zadoks, RN & Dowson, CG 2005 Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates. Journal of Clinical Microbiology 43 4737–4743CrossRefGoogle ScholarPubMed
Struelens, MJ, Deplano, A, Godard, C, Mães, N & Serruys, E 1992 Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis. Journal of Clinical Microbiology 30 2599–2605CrossRefGoogle ScholarPubMed
Zadoks, RN, van Leeuwen, W, Barkema, H, Sampimon, O, Verbrugh, H, Schukken, YH & van Belkum, A 2000 Application of pulsed-field gel electrophoresis and binary typing as tools in veterinary clinical microbiology and molecular epidemiologic analysis of bovine and human Staphylococcus aureus isolates. Journal of Clinical Microbiology 38 1931–1939CrossRefGoogle ScholarPubMed