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Outer membrane characteristics of Salmonella enteritidis phage type 4 growing in chickens

Published online by Cambridge University Press:  15 May 2009

H. Chart ,
D. Conway  and
B. Rowe
H. Chart
Affiliation:
Laboratory of Enteric Pathogens
D. Conway
Affiliation:
Biological Services Department, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens
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Summary

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Strains of Salmonella enteritidis belonging to phage type 4 (SE4) were grown in the peritoneal cavities of chickens, and without subculture on laboratory media examined for inducible in vivo phenotypic characteristics. These bacteria expressed three major outer membrane proteins (OMPs) of 33, 35 and 36 kilodaltons (kDa), and iron regulated OMPs of 74, 78 and 81 kDa. Bacteria growing in vivo did not express flagella, or fimbriae with a subunit molecular mass of 14 kDa (14 kDa fimbriae). Two OMPs of 55 and 23 kDa, expressed during culture in nutrient broth, were repressed during growth in chickens. Possession of a 38 MDa ‘mouse virulence’ plasmid did not influence the expression of OMPs, flagella or fimbriae. It was concluded that strains of SE4 growing in chicken tissues, use an enterobactin mediated iron uptake system to obtain ferric ions, do not express flagella or 14 kDa fimbriae and appear not to express novel OMPs involved in survival in vivo.

Information

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 3 , December 1993 , pp. 449 - 454
Copyright
Copyright © Cambridge University Press 1993

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