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The Effects of electromagnetic field exposure at 900 mHz frequency emitted from mobile phones on cochlear cells

Presenting Author: Yüksel Olgun

Published online by Cambridge University Press:  03 June 2016

Safiye Aktaş ,
Zekiye Altun ,
Ayça Pamukoğlu ,
Banu Demir ,
Serkan Saygan ,
Burak Çiçeksoy ,
Pınar Erçetin ,
Efe Serinan ,
Yüksel Olgun  and
Nur Olgun
Safiye Aktaş
Affiliation:
Dokuz Eylül University Institute of Oncology Department of Basic Oncology
Zekiye Altun
Affiliation:
Dokuz Eylül University Institute of Oncology Department of Basic Oncology
Ayça Pamukoğlu
Affiliation:
Dokuz Eylül University Institute of Oncology Department of Basic Oncology
Banu Demir
Affiliation:
Dokuz Eylül University Institute of Oncology Department of Basic Oncology
Serkan Saygan
Affiliation:
Derinuzay Science and Techology Labs R&D Assc
Burak Çiçeksoy
Affiliation:
University of Economics Deparment of Electric and Electronical Engineering
Pınar Erçetin
Affiliation:
Dokuz Eylül University Institute of Oncology Department of Basic Oncology
Efe Serinan
Affiliation:
Dokuz Eylül University Institute of Oncology Department of Basic Oncology
Yüksel Olgun
Affiliation:
Dokuz Eylül UniversitySchool of Medicine Department of Otorhinolaryngology
Nur Olgun
Affiliation:
Dokuz Eylül University, Institute of Oncology
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Abstract

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Type
Abstracts
Information
The Journal of Laryngology & Otology , Volume 130 , Supplement S3: Abstracts for the 10th International Conference on Cholesteatoma , May 2016 , pp. S150
Copyright
Copyright © JLO (1984) Limited 2016 

Learning Objectives:

Aim: Technological developments encountered radiofrequency field from mobile phones in our lives. Possible side effects of electromagnetic field (EMF) need to be investigated. The aim of this study is to evaluate cytotoxic, apoptotic and DNA damage effects of 900 MHz EMF emitted by mobile phones on House Ear Institute- Organ of Corti 1 (HEI-OC1 cell line) cochlear cells.

Methods: Cochlear cells were cultured in 6 well plates at 33°C, 10%CO2 in humidified conditions. They were exposed to 900 Mhz EMF in conditions of 5 minutes and 15 minutes, directly and 10 cm away from EMF. EMF was applied by a 3 G cell phone and measured by Arduino EMF detector. Cell viability and apoptosis were evaluated after 24 and 48 hours after exposure for each condition and control group by trypan blue and Annexin V methods respectively. DNA damage related gene expressions was evaluated by real time PCR after RNA isolation and cDNA synthesis.

Results: Cell death was more prominent in cells which received 5 minutes of EMF at 48 h. The apoptosis ratio in cells situated 10 cm away from EMF were similar to cells that were directly exposed to 5 minutes of EMF. In gene expression results it was observed that DNA damage related gene expressions were increased in cells after EMF exposure in 48 hours. The expression levels are nearly same in cells that were 10 cm away from EMF. The genes that showed high expression than control are Bax, Gadd45a, Gadd45 g, Mpg, Msh2, Rad51c and Xrcc3, which are related to apoptosis induction and DNA repair.

Conclusion: EMF at high dose for 5 minutes caused cell death via apoptosis in HEI-OC1 cell line in vitro. This result was supported by apoptosis detection and DNA damage related gene expressions. Apoptosis was prominent in 5 minutes and similar for both direct and close distance exposure. Further in vivo and in vitro studies with different doses and distances are needed to evaluate the effects of EMF on cochlea.