Introduction
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), also known as coronavirus disease (COVID-19), was first identified in Wuhan, China, in late 2019 and rapidly spread worldwide [Reference Mallapaty1]. Governments implemented travel regulations and public health measures, including entry screening, masking, physical distancing, and lockdowns, which helped mitigate regional transmission [Reference Chinazzi2,Reference Gwee3]. Remote and isolated regions faced particular challenges during the pandemic, highlighting the need for context-specific, targeted preventive measures. Remote Japanese islands are especially vulnerable to infectious diseases owing to limited healthcare infrastructure and geographical isolation, amplifying the potential impact of outbreaks.
Comparable challenges were observed elsewhere. In Mali, authorities struggled to contact positive cases, lacked isolation facilities, and reported behavioural changes among displaced populations [Reference Gwee3]. In contrast, many US-affiliated Pacific Islands implemented early and strict border and quarantine measures, which helped delay community transmission, allowing time to establish testing capacity and achieve high immunization coverage. Guam and the Commonwealth of the Northern Mariana Islands maintained open borders but enforced mandatory quarantine protocols for arriving travellers, which also contributed to delaying widespread transmission during the early stages of the pandemic [Reference Cash McGinley4]. Primary strategies to prevent COVID-19 transmission included vaccination, travel restrictions, and diagnostic testing [Reference Girum5]. Polymerase chain reaction (PCR) testing, a cornerstone of SARS-CoV-2 detection [Reference Hay6], enables the rapid identification of infected individuals for isolation, which may help limit viral spread. Despite its recognized utility, the application of PCR testing in managing or monitoring COVID-19 transmission on remote islands remains understudied [Reference Terada-Hirashima7].
Although extensive research has focused on COVID-19 prevention, a significant gap exists in the evaluation of voluntary PCR testing among travellers to remote Japanese islands. Few studies have addressed the unique challenges faced by these islands, leaving key uncertainties regarding vulnerability and potential consequences. In a global context where the effectiveness of public health strategies is paramount, this study is particularly relevant for safeguarding vulnerable populations. Therefore, we examined the implementation and outcomes of voluntary pre-boarding PCR testing among travellers to remote Japanese islands and explored its association with reported infection trends, aiming to provide evidence to inform future infectious disease preparedness.
Methods
Study population and sample collection protocol
The Ogasawara Islands are located approximately 1,000 km from mainland Japan and are accessible only by a weekly 24-h ferry service. This study included travellers aged ≥6 years going from the mainland to Ogasawara; returnees were excluded. Participants voluntarily submitted saliva samples to the SB Coronavirus Inspection center Corp. the day before travel.
Case determination and data collection
Travellers received ZEESAN Saliva RNA Sample Collection Kits. Samples collected 24–96 h before departure were analysed at the SB Coronavirus Inspection center Corp. (Tokyo, Japan) using the SARS-CoV-2 Direct Detection RT-qPCR Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions. Positive results (Ct < 40) led to travel prohibition and referral for medical care. COVID-19 case data for Tokyo and other islands were obtained from official websites [8–14]. Entry restrictions and control measure data were obtained from the authorities and shipping companies. No pre-departure tests were conducted on other islands.
Descriptive epidemiology
This observational study utilized a survey using data derived from a testing programme implemented as part of Tokyo’s infection control policies. Ethics approval allowed the secondary use of data with online disclosure and opt-out. Demographic and health data (age, sex, current diseases, vaccination history, and testing awareness) were collected via a questionnaire. Disease and attitude data were collected from May 2021 to September 2022; vaccination history was collected from May to September 2022; the average stay was 1.5–4.0 days, based on a major Japanese travel agency itinerary [15–17].
Ethics approval
The procedures complied with national and institutional committees on human experimentation and with the Declaration of Helsinki (1975, revised in 2008). Sample collection ensured anonymity. Study details and opt-out information were provided on the hospital website. For minors, refusals from the participant or guardian were honoured. The study was approved by the Institutional Review Board of the National center for Global Health and Medicine (approval number: NCGM-G-003678-00; 5 August 2020).
Statistical analysis
Descriptive statistics are presented as medians (interquartile ranges) or counts (percentages). Incidence rates per 1,000 person-days were calculated for each island and study period. We implemented an island-by-period panel comparison, focusing on three predefined intervals reflecting policy and epidemiological contexts: (1) the entire study period (September 2020–September 2022), (2) the pre-Omicron phase (September 2020–May 2022), and (3) the 7th wave dominated by the Omicron BA.5 lineage (June–September 2022). For each interval, the incidence rate ratio (IRR) for each island was calculated using Ogasawara as a reference, and the corresponding 95% confidence interval (CI) was calculated assuming a Poisson distribution. The testing rate ratio and corresponding 95% CI values were estimated using a modified Poisson regression model [Reference Zou18]. Statistical analyses were performed using SPSS (version 25; IBM Corp., Armonk, NY, USA) or R version 4.3.1 (R Foundation for Statistical Computing, Vienna, Austria).
Results
Characteristics of travellers
Between September 2020 and September 2022, 45,900 individuals travelled to the Ogasawara Islands. Of these, 38,943 (86.7%) eligible individuals aged ≥6 years willingly provided specimens and participated in the questionnaire survey (Table 1). The median age of the travellers was 43 years, with 5,187 (13.3%) aged ≥65 years, indicating an elevated risk of severe illness. The sex distribution was 61% male and 38.4% female, with travel motives including tourism (56.2%), business (23.1%), and residence (13.4%). Notably, 13.6% had health conditions that posed potential risks. From May to September 2022, 85.4% of the participants were vaccinated.
Demographic and general characteristics of travellers to Ogasawara
a The information on diseases under treatment and attitudes towards testing was collected between May 2021 and September 2022, and that on vaccination history was collected only between May 2022 and September 2022.
b Refers to the actual number of passengers onboard, including those aged <6 years.
c All prospective passengers, except those aged <6 years, were considered for testing.
d Indicates the number of specimens actually received from individuals subject to testing. Passengers who cancelled boarding or were not allowed to board because of positive screening test results were included. Passengers who cancelled for other reasons were excluded.
e IQR, interquartile range.
f When counting ‘Symptoms’, ‘Reason for Visit’, and ‘Currently Treated Disease’, multiple answers were accepted.
qPCR, quantitative polymerase chain reaction.
Infection trends in Ogasawara
During the study period (1 September 2020–30 September 2022), Ogasawara Village reported 385 positive cases, of which 44 involved individuals aged >60 years (Figure 1). The highest daily caseload in Ogasawara, 25 cases, was recorded on 29 July 2022. In contrast, Tokyo reported a total of 3,147,331 cases across Waves 2–7, leading to 1,481,307 (47.1%) hospitalizations and 41,947 (1.3%) severe cases. Ogasawara Village reported neither hospitalizations nor severe cases during the same period.
Patients with COVID-19 in Ogasawara and Tokyo. The pink bars indicate COVID-19 patients in Ogasawara, and the purple bars indicate the individuals who tested positive for COVID-19 before boarding to Ogasawara between 1 September 2020 and 30 September 2022. The green line indicates the number of passengers travelling from Tokyo to Ogasawara, and the blue line indicates the testing rate of saliva samples using PCR before boarding the vessel. Orange bars indicate COVID-19 cases in Tokyo between 1 September 2020 and 30 September 2022. The period of the declaration of the state of emergency is indicated by pink blocks. The main prevalent variants are indicated by arrows. COVID-19: coronavirus disease.
Monthly incidence rates per 1,000 person-days were calculated for Ogasawara and other Tokyo islands to visualize temporal fluctuations (Figure 2a). Distinct seasonal and wave-specific patterns were observed, with Ogasawara showing consistently low rates, except during the nationwide 7th wave (June–September 2022).
(a) Temporal trends in incidence rates of COVID-19 on the Tokyo islands, calculated based on the number of days visitors stayed on each island. (b) Comparison of incidence rates per 1,000 person-days for Ogasawara and other Tokyo islands during three periods: the entire study period (September 2020–September 2022), the pre-Omicron phase (September 2020–May 2022), and the 7th wave dominated by the Omicron BA.5 lineage (June–September 2022). Each bar indicates the infection rate per 1,000 person-days. The left, middle, and right figures show the infection rates for all periods from September 2020 to May 2022 and from June 2022 to September 2022, respectively. The IRR for each island was calculated using Ogasawara as a reference, and the corresponding 95% confidence interval (95% CI) was calculated by assuming a Poisson distribution. IRR: incidence rate ratio; CI: confidence interval.
IRRs (cases per 1,000 person-days) were estimated for three predefined periods: the entire study period (September 2020–September 2022), the pre-Omicron phase (September 2020–May 2022), and the 7th wave dominated by Omicron BA.5 (June–September 2022) (Figure 2b, Table S1). During the pre-Omicron phase, all islands except Niijima and Miyakejima showed significantly higher IRRs than Ogasawara, whereas the differences diminished during the 7th wave.
Pre-boarding qPCR testing
From 1 September 2020 to 30 September 2022, 38,943 individuals underwent pre-travel PCR testing, representing 86.7% of those eligible (Table 1). Test participation increased to 93.8% during the emergency declaration period, compared with 84.7% in the remainder of the study (p < 0.001; Figure 3). Among all individuals who underwent testing during the study period, 148 who tested positive and did not travel (Figure 1) were not followed up. The median body temperature at the time of saliva collection was 36.3 °C, and 97.8% of those tested were asymptomatic (Table 1). Symptomatic cases included cough and phlegm (0.5%), fatigue (0.1%), olfactory impairment (0.1%), and dysgeusia (0.1%). Although most individuals were asymptomatic, those with positive pre-boarding test results had a higher proportion of symptoms (10.8%), particularly coughing and phlegm expression (7.4%). Most respondents reported that the test was easy to perform, necessary, and reassuring for safe travel.
Boxplot of testing rates during and after the Japanese government declared a state of emergency in response to the surge in coronavirus infections. The orange and blue circles indicate the testing rate for each week using PCR before boarding the vessel, and the × mark indicates the mean testing rate.
Comparative analysis
In this island-by-period panel comparison, Ogasawara, where pre-departure testing was implemented, generally maintained lower incidence rates than several other comparison islands (Figure 2b, Table S1). These findings suggest an association between widespread testing uptake and the lower incidence rates observed in Ogasawara. However, the observational design did not allow causal inference regarding subsequent community transmission.
Discussion
We investigated the association between voluntary pre-boarding PCR testing among travellers to Ogasawara and reported island-level infection trends from September 2020 to September 2022. We observed that periods with higher uptake of pre-boarding PCR testing were associated with lower reported infection rates in Ogasawara compared with those in several other Tokyo islands. Because this was an observational study conducted in the presence of multiple concurrent public health measures, causal relationships cannot be inferred.
The high testing rate of 86.7% over the entire period, particularly in emergency situations (93.8%), emphasizes the feasibility and acceptability of this preventative measure. Testing is an important component of COVID-19 control, yet barriers and hesitancy can affect participation rates [Reference Embrett19]. Local government coverage of test costs, combined with the use of self-collected saliva samples, likely enhanced acceptance. The same-day delivery of results by private companies may also have increased uptake.
Fortunately, 80% of the individuals who contracted COVID-19 in 7th Wave had already received two doses of the vaccine. Most cases during the Omicron-dominant period were mild, and no severe disease was reported in Ogasawara.
In Ogasawara, pre-boarding PCR testing was voluntary; thus, some of the 14% of travellers who did not undergo PCR testing prior to travel may have been infected. Schneitler et al. reported similarly low SARS-CoV-2 positivity in Germany during high transmission, partly due to travellers’ self-isolation 7–10 days before testing to avoid trip cancellations and costs [Reference Schneitler20]. Such behaviour may have occurred in Ogasawara; however, voluntary testing likely missed some infections, especially during surges in Tokyo. Schneitler et al. further noted that mandatory testing aids in detection and engages traveller groups otherwise unlikely to seek pre-travel medical advice – benefits not realized in Ogasawara’s voluntary system [Reference Schneitler20].
In 7th Wave, 77.08% of the Japanese population had completed a second vaccine dose as of 2 July 2022; however, the third-dose rate was still low at 62.60% and may have been even lower among younger people [21]. The average age of travellers was 46 years. Individuals who had received the second vaccination and believed themselves not to have COVID-19 might still have been infected. However, voluntary testing may result in missing infections, especially among asymptomatic individuals or during surges, and the potential advantages of mandatory testing – such as broader and more uniform coverage across traveller groups – were not realized in this setting.
Notably, no hospitalizations or severe cases were reported in Ogasawara despite the substantial number of travellers. During the pandemic, medical facilities initially transported patients with COVID-19 to Tokyo by helicopter. Subsequently, individuals with mild disease were managed through home- or accommodation-based isolation to avoid unnecessary hospitalization. Rapid PCR testing after symptom onset and voluntary isolation systems may have aided timely case management. These experiences illustrate how the Ogasawara healthcare system adapted to the constraints of a remote island setting during the COVID-19 pandemic. Local antigen and PCR testing were important for rapid identification and isolation, representing an adaptive approach to patient management. Unlike the previous practice of transporting patients to Tokyo, the revised strategy emphasized home or facility isolation of mild cases, optimizing local resources.
Even during the pandemic, tourism (56.2%) was the main reason for travel, followed by business (23.1%), residence (13.4%), and returning home (4.6%). The local government implemented several measures to mitigate transmission risk. Residents and tourists were urged to refrain from unnecessary outings after 8:00 PM. To enhance safety, individuals who underwent pre-travel testing were provided with wristbands as visible proof of testing. Tourist facilities prioritized those wearing wristbands, and restaurants adjusted operational hours to reduce service times. Vessel capacity, the exclusive mode of transport to Ogasawara, was restricted by 30–50% to ensure passenger spacing. These measures were implemented concurrently to reduce transmission risk while sustaining essential economic activity, and their individual effects could not be elucidated in this analysis.
The geographical context of Ogasawara – located approximately 1,000 km from mainland Japan and accessible only by a weekly 24-h ferry – provides a unique setting for examining infection trends (Supplementary Figure S1). Travellers spend 2–3 days longer on Ogasawara than on other Tokyo metropolitan islands. Despite longer stays, the infection rates in Ogasawara were similar to or lower than those in other islands. In particular, during the pre-Omicron phase (September 2020–May 2022), IRRs for all islands, except Miyakejima and Niijima, were significantly higher than those for Ogasawara, whereas differences diminished during the 7th wave (June–September 2022).
Several limitations of the study should be considered. It was observational, and we could not control for concurrent public health measures, behavioural changes, or vaccination trends. In addition, outbreak seeding and sustained transmission in small island populations can be highly stochastic, and the observed incidence is strongly influenced by chance contact patterns and low absolute case numbers. Saliva samples were self-collected, potentially increasing the risk of improper collection and false-negative PCR results. The sensitivity and specificity of the saliva test were 86.4% (95% CI: 82.8–89.4%) and 97.0% (95% CI: 95.0–98.3%), respectively, which could result in false negatives during early infection [Reference Zhu22]. Previous studies indicate that increasing screening frequency is more effective than improving test accuracy in preventing infection [Reference Kucirka23]. More detailed information on the infection status of travellers after arrival is required to rigorously evaluate the association between pre-boarding testing and island-level transmission. Additionally, the healthcare system may not have identified all COVID-19 cases. Travellers were advised to undergo PCR testing prior to travel and to wear wristbands as proof, suggesting that they acted cautiously while on the island. Although details of household composition were unavailable, close household contact may have facilitated local transmission. Such contextual data would enable more refined analysis and inform infection control measures. Future studies should consider the quantitative evaluation of vaccination efficacy and cost-effectiveness, as well as the behavioural context of testing.
In conclusion, voluntary pre-boarding PCR screening was associated with lower reported infection rates in Ogasawara during the study period. However, causal relationships could not be established owing to the observational study design, concurrent interventions, and the stochastic nature of transmission in small populations. Nevertheless, the findings underscore the potential value of integrated approaches that combine accessible testing, vaccination, and adaptive local healthcare systems in preparing for future infectious disease threats in remote settings.
Supplementary material
The supplementary material for this article can be found at http://doi.org/10.1017/S0950268826101289.
Data availability statement
Not applicable.
Acknowledgements
We thank all the study participants; Yoshida Shigeru and Yusuke Takahashi of the Health Sciences University of Hokkaido; the staff of the SB Coronavirus Inspection center Corp.; Masaaki Shibuya, Mayor of Ogasawara Village, and village office staff; and the employees of Ogasawara Kaiun Co. Ltd. for their study support. We also thank Editage for English language editing.
Author contribution
M.K.: Conceptualization, Data curation, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing; Y.A.: Formal analysis, Writing – original draft, Writing – review & editing; S.T.: Formal analysis, Writing – review & editing; Y.S.: Formal analysis, Writing – original draft, Writing – review & editing; Y.U.: Formal analysis, Writing – original draft, Writing – review & editing; Y.T.: Data curation, Investigation, Methodology; J.T.-H.: Conceptualization, Data curation, Investigation, Methodology, Project administration; M.I.: Investigation, Methodology; Y.K.: Data curation; M.I.: Project administration, Resources; T.K.: Data curation, Project administration, Resources; N.O.: Conceptualization, Project administration, Resources; W.S.: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing.
Funding statement
This study was supported by Japan Institute for Health Security (23300121 to M.K.) and JSPS KAKENHI (JP21 K17321, JP21H04595, JP23 K01288, JP24 K13477 to Y.A.). Funders had no role in the study design, data collection, analysis, interpretation, writing, or publication.
Competing interests
Wataru Sugiura received research grants from the SB Coronavirus Inspection Centre center Corp. outside the submitted work. The remaining authors declare no conflicts of interest.
Ethical approval statement
This survey was part of Tokyo’s infection control measures. Written informed consent was not obtained. The ethics committee approved the secondary use of the Tokyo survey results on the condition that study details were posted online and an opt-out option was provided. The Institutional Review Board of the National center for Global Health and Medicine approved the study (approval number: NCGM-G-003678-00, 5 August 2020). All saliva samples were processed to protect the participants’ identities. Study information, including possible use of records for research, was posted on the hospital website with an option to decline. For minors, refusals by the participant or their legal guardian were accepted.
Use of artificial intelligence (AI) tools
AI tools were used only to assist with proofreading and improving expressions. They were not used in developing the study concept, designing analyses, generating results, or interpreting data. All intellectual content and conclusions remain the authors’ responsibility.
Open access
