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Wolbachia does not give an advantage to the ectoparasitoid Habrobracon hebetor (Say, 1836) when it develops on an infected host

Published online by Cambridge University Press:  30 January 2025

Natalia A. Kryukova Open the ORCID record for Natalia A. Kryukova [Opens in a new window] ,
Olga V. Polenogova ,
Ulyana N. Rotskaya ,
Karina A. Zolotareva  and
Ekaterina A. Chertkova
Natalia A. Kryukova*
Affiliation:
Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia
Olga V. Polenogova
Affiliation:
Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia
Ulyana N. Rotskaya
Affiliation:
Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia
Karina A. Zolotareva
Affiliation:
Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia
Ekaterina A. Chertkova
Affiliation:
Laboratory of ecological parasitology, Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia
*
Corresponding author: Natalia A. Kryukova; Email: dragonfly6@yandex.ru
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Abstract

The effect of Wolbachia on the viability and antimicrobial activity of the ectoparasitoid Habrobracon hebetor was evaluated in laboratory experiments. Two lines of the parasitoid, Wolbachia-infected (W+) and Wolbachia-free (W−), were used. Parasitoid larvae were fed with a host orally infected with a sublethal dose of Bacillus thuringiensis (Bt) and on the host uninfected with Bt. Parasitoid survival was assessed at developmental stages from second-instar larvae to adults. At all developmental stages, there were no statistically significant differences in survival between lines W+ and W−, regardless of host Bt infection. In both W+ and W− lines, the expression of lysozyme-like proteins, antimicrobial peptides (AMPs), and Hsp70 genes was analysed in fourth-instar larvae fed with an infected and uninfected host. In addition, lysozyme-like activity and antibacterial activity were evaluated. The expression of AMPs was significantly higher in W− larvae and did not get induced during the feeding on the Bt-infected host. mRNA expression of lysozyme-like proteins and lysozyme activity were significantly higher in W+ larvae than in W− larvae and did not get induced when the larvae were fed with the infected host. In whole-body homogenates of H. hebetor larvae fed with the uninfected host, antibacterial activity against gram-positive bacteria (Bacillus cereus and Bacillus subtilis) was significantly higher in the W+ line and did not get induced during the feeding with the Bt-infected host. Therefore, there is no obvious immunostimulatory effect of Wolbachia in H. hebetor larvae when they feed on a host infected with an entomopathogenic bacterium.

Keywords

bacteriosisHabrobracon hebetorWolbachia

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Type
Research Paper
Information
Bulletin of Entomological Research , Volume 115 , Issue 2 , April 2025 , pp. 131 - 140
Copyright
© The Author(s), 2025. Published by Cambridge University Press.

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