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The development and evaluation of a μ-capture ELISA detecting chlamydia-specific Igm

Published online by Cambridge University Press:  15 May 2009

T. G. Wreghitt ,
V. J. Robinson ,
E. O Caul ,
I. D. Paul  and
S. Gatley
T. G. Wreghitt
Affiliation:
Clinical Microbiology and Public Health Laboratory, Level 6, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW.
V. J. Robinson
Affiliation:
Department of Pathology, University of Cambridge.
E. O Caul
Affiliation:
Joint Regional Public Health Laboratory Service and District Virology Laboratory, Myrtle Road, Kingsdown, Bristol.
I. D. Paul
Affiliation:
Joint Regional Public Health Laboratory Service and District Virology Laboratory, Myrtle Road, Kingsdown, Bristol.
S. Gatley
Affiliation:
Novo BioLabs Ltd., Downham House, Downham's Lane, Milton Road, Cambridge.
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Summary

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A μ-capture enzyme-linked immunosorbent assay (ELISA) for detecting chlamydia-specific IgM was developed by use of the heat stable, lipopolysaccharide group-specific antigen and an alkaline phosphatase-labelled anti-chlamydia group-specific monoclonal antibody conjugate. The test was used to study the serological response in chlamydial respiratory tract infection among patients with acute respiratory tract symptoms in Cambridgeshire during the past 7 years. Results were compared with those of the complement fixation test (CFT) in routine use as well as those of a whole inclusion indirect immunofluorescence (WIF) test for IgM. Correlation between results of the μ-capture ELISA and those of the WIF test was 87·5%.

Information

Type
Research Article
Information
Epidemiology & Infection , Volume 101 , Issue 2 , October 1988 , pp. 387 - 395
Copyright
Copyright © Cambridge University Press 1988

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