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Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy

Published online by Cambridge University Press:  08 June 2015

S. Riis ,
V. Zachar ,
S. Boucher ,
M.C. Vemuri ,
C. P. Pennisi  and
T. Fink
S. Riis
Affiliation:
Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark
V. Zachar
Affiliation:
Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark
S. Boucher
Affiliation:
Thermo Fisher Scientific, Frederick, MD, USA
M.C. Vemuri
Affiliation:
Thermo Fisher Scientific, Frederick, MD, USA
C. P. Pennisi
Affiliation:
Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark
T. Fink*
Affiliation:
Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark
*
* Corresponding author: Trine Fink, Frederik Bajers vej 3B, 9220 Aalborg Ø, Denmark. E-mail: trinef@hst.aau.dk
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Abstract

Since the discovery of adipose-derived stem cells (ASCs), there have been high expectations of their putative clinical use. Recent advances support these expectations, and it is expected that the transition from pre-clinical and clinical studies to implementation as a standard treatment modality is imminent. However ASCs must be isolated and expanded according to good manufacturing practice guidelines and a basic assurance of quality, safety, and medical effectiveness is needed for authorisation by regulatory agencies, such as European Medicines Agency and US Food and Drug Administration. In this review, a collection of studies investigating the influence of different steps of the isolation and expansion protocol on the yield and functionality of ASCs has been presented in an attempt to come up with best recommendations that ensure potential beneficial clinical outcome of using ASCs in any therapeutic setting. If the findings confirm the initial observations of beneficial effects of ASCs, the path is paved for implementing these ASC-based therapies as standard treatment options.

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Type
Review Article
Information
Expert Reviews in Molecular Medicine , Volume 17 , 2015 , e11
Copyright
Copyright © Cambridge University Press 2015 

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