The study provides a radiocarbon sequence for the Iron Age occupation in the elevated areas of the Phoenician settlement of Lisbon, located in the Tagus estuary (Portugal). The dataset is based in ten animal and human samples recovered during archaeological excavations at Largo de Santa Cruz do Castelo. These samples are associated with distinct phases of the Iron Age, dated by the ceramic findings between the 7th and 5th century BCE, as well as a latter sample from the Roman Republican Period (2nd half of the 2nd century BCE). Despite the challenges posed by the 1st millennium BCE radiocarbon calibration, this dataset proves valuable for establishing a more detailed chronological framework. It represents a significant contribution to refining the timeline of Lisbon’s Iron Age settlement and provides a stronger basis for interpreting local developments within the broader regional context.