The adoption of Artificial Intelligence (AI) in the maritime sector marks a significant technological advancement with broad implications for operational efficiency, crewing, and regulatory frameworks. While these innovations are expected to enhance safety, reduce operating costs, and promote environmental sustainability, they are also likely to introduce challenges related to workforce displacement, cybersecurity, and evolving labor regulations at sea. This chapter examines the impact of AI on the maritime workforce, more specifically seafarers. It explores how AI may affect crew size, the emergence of new roles, and new skills in the future. It also offers an analysis of the significant impact of AI on working conditions and labor rights at sea under international maritime regulations, particularly the Maritime Labour Convention (MLC), 2006, and the Standards of Training, Certification and Watchkeeping for Seafarers Convention (1978, STCW Convention), as amended. This chapter explores the intersection areas of AI and maritime law, focusing on the emerging regulatory frameworks, including the EU AI Act and the International Maritime Organization’s Maritime Autonomous Surface Ships (MASS) Code. The findings point out that while AI presents opportunities for improving the working conditions of seafarers, its use must adhere to acceptable labor standards, legal clarity, and robust cybersecurity measures.