Introduction. Chestnut represents one of the most important cropsfor the Portuguese economy. The most common consumption mode ofthese fruits is roasted or boiled. In this context, the major aimof our study was to evaluate the amino acid contents and the mineralcomposition of raw and cooked chestnuts. Materials and methods.Amino acids were determined by HPLC and minerals were determinedby molecular absorption spectrophotometry, by atomic absorptionspectrophotometry or by flame emission photometry. The most importantPortuguese cultivars were evaluated: Aveleira, Boaventura, Côta,Judia, Lada, Lamela, Longal Padrela, Longal Soutos da Lapa, Negraand Martaínha. Results and discussion. The cooking method significantlyaffected the total amino acid composition with contents in roastedsamples 13% and 12 % higher than in boiled and raw chestnuts, respectively.Roasted chestnuts presented higher alanine, arginine, isoleucine,leucine, phenylalanine, threonine, tyrosine and valine contentsthan either raw or boiled chestnuts. Moreover, serine presentedthe highest content in raw or roasted chestnut kernels. Regarding themineral composition, potassium (K) was the predominant macronutrient inthe chestnuts, whereas phosphorus (P), calcium (Ca) and magnesium (Mg)were found in low contents. Cooking significantly affected the mineral composition,except for iron (Fe), copper (Cu), and manganese (Mn) contents.In general, calcium decreased upon cooking. K, Ca, Mg and B decreased withboiling. The present data confirm that cooked chestnuts are a good sourceof amino acids and minerals, both affected by boiling and roasting, whichhave been associated with positive health benefits.