Chapter 7 deals with neuroimaging methods for investigating the structural components underlying brain function. Beginning with lesion-symptom mapping (LSM), which identifies relationships between localized brain damage and specific cognitive deficits, the chapter examines how structural abnormalities correlate with functional impairments. Three primary approaches to measuring brain structures with MRI are discussed: structure tracing for hypothesis-driven volumetric analysis, voxel-based morphometry (VBM) for whole-brain comparison of tissue concentration, and surface-based morphometry (SBM) for analyzing the cortical sheet’s unique properties including thickness, curvature, and gyrification. The chapter then explores diffusion tensor imaging (DTI), a technique that visualizes white-matter tracts by measuring the anisotropic diffusion of water molecules along axon bundles. DTI tractography reveals the brain’s “highways,” short, intermediate, and long-range fiber pathways that connect functional modules within and across hemispheres. Together, these complementary techniques provide critical insights into the structural architecture supporting brain networks, offering a more complete understanding of brain organization when combined with functional imaging methods.