Introduction. In Malaysia, Limau Madu (Citrusreticulata Blanco; Citrus suhuiensis Hort.ex Tanaka) is commercially cultivated for local consumption. Itis a loose-peel mandarin with fruits that are spherical in shapewith shiny green or greenish yellow peel. In Malaysia, Limau Maduis vegetatively propagated, thus it is presumed to exhibit minimalvariability. In our study, the genetic variability and genetic relatednessamong cultivated samples collected from different states in Malaysiawere assessed using Simple Sequence Repeat (SSR) primers. Materialsand methods. Thirty pairs of SSR primers derived from Citrusunshiu were screened and 22 SSR primer pairs were utilizedto assess genetic variability and relatedness among 118 cultivatedsamples. Results and discussion. The percentage of SSR transferabilityfrom C. unshiu to C. reticulata (LimauMadu) was 73.33%, which indicated that the primer sequences flankingsimple sequence repeats were conserved among these Citrus species.Most SSR loci revealed a large excess of heterozygotes. In our study,low allelic diversity was shown, with an average of 5.227 allelesper locus. Polymorphic information content (PIC) ranged from 0.048to 0.674. Based on UPGMA clustering analysis, four groups were identifiedfrom these citrus genotypes with a mean genetic distance of 0.170. Lowgenetic variability within species was probably due to vegetativepropagation or inability to detect differences among samples usingthese primers.

Introduction. Turkey potentially has a very rich source of sweet(Prunus avium) and sour (P. cerasus) cherries.P. avium is apparently native to some parts of Northern Turkey, whereGiresun is located. Identification of the sweet cherry cultivars produced in Turkey willhelp in choosing appropriate cultivars and aid in the preservation of natural resourcesrequired for breeding studies. The most conventional method of cultivar identification isbased on the assessment of morphological characteristics. However, this method isinsufficient to distinguish closely related cultivars. The aims of our study were todetermine the molecular profile of sweet cherry accessions grown in Giresun, Turkey, andto determine their genetic relationships. Materials and methods. In ourstudy, we identified 44 sweet cherry accessions grown in Giresun by using genetic markers(SSR, Simple Sequence Repeat), and we determined the genetic relationships among the sweetcherry genotypes. For DNA isolation, we collected young leaves sampled on a single plantper accession, then amplification of microsatellite loci was performed. In total, ten SSRprimer pairs, previously isolated from peach and sweet cherry, were used. Geneticsimilarity values were calculated. A cluster analysis was performed to generate adendrogram. Results and discussion. Of the ten primers tested, six primerpairs did not result in suitable amplification products with the 44 accessions studied.The remaining four polymorphic SSR primer pairs produced 33 alleles with an average of8.25 putative alleles per locus, ranging from 7 to 11. Depending on the accessions,similarity ratios ranged from 0.32 to 0.98, with a mean value of 0.64. In conclusion, theresults obtained demonstrate a high level of polymorphism among sweet cherry genotypesfrom a single province in Turkey.