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Hypoxia combined with chilling maintains the quality of irradiated Drosophila flies: a simulated shipment experiment

Published online by Cambridge University Press:  25 August 2021

Thomas Enriquez Open the ORCID record for Thomas Enriquez [Opens in a new window] ,
Fabiana Sassù Open the ORCID record for Fabiana Sassù [Opens in a new window] ,
Carlos Cáceres Open the ORCID record for Carlos Cáceres [Opens in a new window]  and
Hervé Colinet Open the ORCID record for Hervé Colinet [Opens in a new window]
Thomas Enriquez
Affiliation:
University of Rennes, CNRS, ECOBIO [(Ecosystèmes, biodiversité, évolution)], UMR 6553, F-35000 Rennes, France
Fabiana Sassù
Affiliation:
Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Wagramerstrasse 5, PO Box 100, 1400 Vienna, Austria Department of Forest and Soil Sciences, Boku, University of Natural Resources and Life Sciences, Vienna, Austria
Carlos Cáceres
Affiliation:
Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Wagramerstrasse 5, PO Box 100, 1400 Vienna, Austria
Hervé Colinet*
Affiliation:
University of Rennes, CNRS, ECOBIO [(Ecosystèmes, biodiversité, évolution)], UMR 6553, F-35000 Rennes, France
*
Author for correspondence: Hervé Colinet, Email: herve.colinet@univ-rennes1.fr
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Abstract

Drosophila suzukii is an invasive fruit pest in Europe and America. Females lay eggs into mature fruit that larvae consume causing important losses. Sterile insect technique (SIT) is under development to control this pest. The efficiency of this technique relies on insect quality. However, during the process from mass production to field release, several steps may compromise insect quality and therefore SIT success. Shipment of sterile insects after irradiation is a key step of SIT programmes. Generally, insects are shipped as pupae and conditions during transport need to be adapted to prevent emergence before field release, while guaranteeing insect quality. To do so, transport is usually performed under low temperature, hypoxia or a combination of both. However, the impact of multiple stressors such as irradiation followed by chilling combined with hypoxia is poorly described and has not been studied in D. suzukii. Therefore, the aim of this study was to simulate a shipment of D. suzukii pupae (irradiated or not) under different conditions (chilling combined or not with hypoxia) for various durations, and to assess consequences on emerged adults. Irradiation followed by hypoxia and/or chilling only weakly altered emergence. However, 48 h of hypoxia without chilling altered the flight ability of flies whether or not they were irradiated. Conversely, when hypoxia was combined with chilling, flight ability remained similar to that of untreated flies. The use of chilling in combination with hypoxia for 48 h could be implemented as a transportation method for SIT programme on D. suzukii.

Keywords

Drosophila suzukiiinsect qualitylow temperaturesterile insect techniquetransport

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Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 6 , December 2021 , pp. 645 - 657
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors made equal contributions to the publication.

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