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The complete mitochondrial genome of Brachytarsina amboinensis (Diptera: Hippoboscoidea: Streblidae) provides new insights into phylogenetic relationships of Hippoboscoidea

Published online by Cambridge University Press:  18 December 2024

Jinting Yang Open the ORCID record for Jinting Yang [Opens in a new window] ,
Wang Yujuan ,
Huijuan Yang  and
Xiaobin Huang Open the ORCID record for Xiaobin Huang [Opens in a new window]
Jinting Yang
Affiliation:
Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors, Dali University, Dali, Yunnan, China
Wang Yujuan
Affiliation:
Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, China
Huijuan Yang
Affiliation:
Department of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou, China
Xiaobin Huang*
Affiliation:
Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Institute of Pathogens and Vectors, Dali University, Dali, Yunnan, China
*
Corresponding author: Xiaobin Huang; Email: huangxb633@nenu.edu.cn
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Abstract

The family Streblidae is a significant grouping of dipteran insects within the superfamily Hippoboscoidea, which parasitizes the body surface of bats. With the global spread of bat-related pathogens in recent years, Streblidae has gained increasing attention due to its potential for pathogen transmission. A sample of Brachytarsina amboinensis was sequenced on the B. amboinensis were obtained, compared with available Streblidae mitogenomes, and the phylogeny of Hippoboscoidea was reconstructed. The results indicate that the mitochondrial genome of B. amboinensis exhibits a relatively high degree of conservation, with an identical gene count, arrangement, and orientation as the ancestral insect's genome. Base composition analysis revealed a strong bias towards A and T in the base composition. Selection pressure analysis indicated strong purifying selection acting on cox1. Pairwise genetic distance analysis showed that cox1 evolved at a relatively slow rate. Regarding phylogenetic relationships, the constructed phylogenetic trees using Bayesian inference and Maximum Likelihood methods supported the monophyly of the Hippoboscoidea, Glossinidae, Hippoboscidae, and Nycteribiidae clades, with high nodal support values. Our research confirmed the paraphyly of the families Streblidae. In the familial relations between Nycteribiidae and Streblidae, New World Streblidae share a closer kinship with Nycteribiidae. This contrasts with prior findings which indicated that Old World Streblidae share a closer kinship with Nycteribiidae. This study not only enhances the molecular database for bat flies but also provides a valuable reference for the identification and phylogenetic analysis of Streblidae.

Keywords

bat fliesBrachytarsina amboinensiscomparative analysisHippoboscoideamitogenomeStreblidae

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Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 6 , December 2024 , pp. 836 - 846
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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