Hostname: page-component-76d6cb85b7-lrvh5 Total loading time: 0 Render date: 2026-07-13T12:54:45.398Z Has data issue: false hasContentIssue false
  • English
  • Français

Acid-soluble nucleotides of human milk at different stages of lactation

Published online by Cambridge University Press:  01 June 2009

Angel Gil  and
Fermin Sanchez-Medina
Angel Gil
Affiliation:
Departamento de Investigación de UNIASA, GranadaSpain
Fermin Sanchez-Medina
Affiliation:
Departamento Interfacultativo de Bioquímica, Universidad de Granada, Granada, Spain
Get access Rights & Permissions [Opens in a new window]

Summary

The acid-soluble ribonucleotides of human milk were measured by enzymic and ion-exchange chromatographic procedures at different stages of lactation. Human colostrum and milk contained CMP, AMP, GMP, UMP, GDP-mannose, UDP-N-acetyl-glucosamine, UDP-N-acetyl-galactosamine, UDP-glucose, UDP-galactose, UDP and other minor nucleotides. Cytidine and adenosine derivatives were present in relatively higher amounts than in milk from ruminant species. The nucleotide concentration decreased with advancing lactation. Human milk at 3 months of lactation contained about 10 µmol/100 ml nucleotides, which represented about 75% of the amount of nucleotides present in human colostrum. Human milk did not contain orotic acid. The UDP-glucose/UDP-galactose ratio was constant during lactation and was similar to that of milks from ruminant species.

Information

Type
Original Articles
Information
Journal of Dairy Research , Volume 49 , Issue 2 , May 1982 , pp. 301 - 307
Copyright
Copyright © Proprietors of Journal of Dairy Research 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

Denamur, R., Fauconneau, G. & Guntz, G. 1959 Les nucléotides acido-solubles des laits de brebis, vache, chévre et truie. Revista Española de Fisiología 15 301–310Google Scholar
Deutsch, A. & Mattsson, S. 1960 Acid-soluble nucleotides in cow's milk and colostrum. Milk and Dairy Research, Report No. 63, Alnarp, Sweden.Google Scholar
Gil, A. & Sánchez-Medina, F. 1981 a Acid-soluble nucleotides of cow's, goat's, and sheep's milks at different stages of lactation. Journal of Dairy Research 48 35–44Google Scholar
Gil, A. & Sánchez-Medina, F. 1981 b The determination of acid-soluble nucleotides in milk by improved enzymic methods: a comparison with the ion-exchange column chromatography procedure. Journal of the Science of Food and Agriculture 32 1123–1131Google Scholar
Hurlbert, R. B., Schmitz, H., Brumm, A. F. & Potter, V. R. 1954 Nucleotide metabolism. II. Chromatographic separation of acid-soluble nucleotides. Journal of Biological Chemistry 209 23–39CrossRefGoogle ScholarPubMed
Johke, T. & Goto, T. 1962 Acid-soluble nucleotides in cow's and goat's milk. Journal of Dairy Science 45 735–741CrossRefGoogle Scholar
Kobata, A. 1963 The acid-soluble nucleotides of milk. II. Isolation and identification of two novel uridine nucleotide oligosaccharide conjugates from human milk and colostrum. Journal of Biochemistry 53 167–175CrossRefGoogle ScholarPubMed
Kobata, A. 1966 The acid-soluble nucleotides of milk. IV. The chemical structure of UDP-X3. Journal of Biochemistry 59 63–66Google Scholar
Kobata, A., Ziro, S. & Kida, M. 1962 The acid-soluble nucleotides of milk. I. Quantitative and qualitative differences of nucleotide constituents in human and cow's milk. Journal of Biochemistry 51 277–287Google Scholar
Larson, B. L. & Hegarty, H. M. 1979 Orotic acid in milks of various species and commercial dairy products. Journal of Dairy Science 62 1641–1644CrossRefGoogle Scholar
Möllering, H. 1974 Orotate: Determination with 0-5-MP pyrophosphorylase. In Methods of Enzymatic Analysis, 2nd Edn Vol. IV pp. 1959–1963 (Ed. Bermeyer, H. U.) New York: Academic PressCrossRefGoogle Scholar
Münchberg, F., Tsompanidou, G. & Leskova, R. 1971 [Studies on the presence of orotic acid in milk.] Milchwissenschaft 26 210–214Google Scholar
Robinson, J. L. 1980 Bovine milk orotic acid: variability and significance for human nutrition. Journal of Dairy Science 63 865–871Google Scholar
Rosenbloom, F. M. & Seegmiller, J. E. 1964 An enzymatic spectrophotometric method for determination of orotic acid. Journal of Laboratory and Clinical Medicine 63 492–500Google ScholarPubMed
Standerfer, S. B. & Handler, P. 1955 Fatty liver induced by orotic acid feeding. Proceedings of the Society of Experimental Biology and Medicine 90 270–271CrossRefGoogle ScholarPubMed