Hostname: page-component-8448b6f56d-gtxcr Total loading time: 0 Render date: 2024-04-16T21:41:52.404Z Has data issue: false hasContentIssue false
  • English
  • Français

Opisthorchis viverrini-like liver fluke in birds from Vietnam: morphological variability and rDNA/mtDNA sequence confirmation

Published online by Cambridge University Press:  31 May 2013

T.H. Dao ,
T.G. Nguyen ,
B. Victor ,
S. Gabriël  and
P. Dorny
T.H. Dao
Affiliation:
Department of Parasitology, National Institute of Veterinary Research, Hanoi, Vietnam
T.G. Nguyen
Affiliation:
Ministry of Public Health, Vietnam
B. Victor
Affiliation:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Belgium
S. Gabriël
Affiliation:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
P. Dorny*
Affiliation:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Belgium
*
* E-mail: pdorny@itg.be
Get access Rights & Permissions [Opens in a new window]

Abstract

Flukes were found in the bile ducts of domestic ducks (Anas platyrhynchos), necropsied in the Binh Dinh province of Central Vietnam. Following staining, morphological characteristics of the bird flukes were compatible with Opisthorchis viverrini, although some characteristics differed from those described in specimens collected from mammal hosts. Computation of the phylogenetic trees on the partial sequences of the second internal ribosomal spacer (ITS2) of the ribosomal DNA and cytochrome c oxidase subunit I (COI) markers of the mitochondrial DNA showed close similarity of the ‘bird’ Opisthorchis sp. with O. viverrini. We speculate that these bird flukes are O. viverrini that show intraspecies morphological and molecular variability compared to isolates from mammals. This demonstrates the complex epidemiological situation of opisthorchiasis in Vietnam and urges investigations on the potential of birds as a reservoir host of this zoonotic fluke.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 4 , December 2014 , pp. 441 - 446
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anh, N.T. (2009) Fishborne zoonotic trematodes in domestic animals and their role in the transmission to cultured fish in North Vietnam.PhD Thesis, Department of Veterinary Disease Biology, University of Copenhagen, Denmark.Google Scholar
Anh, N.T., Madsen, H., Dalsgaard, A., Phuong, N.T., Thanh, D.T. & Murrell, K.D. (2010) Poultry as reservoir hosts for fishborne zoonotic trematodes in Vietnamese fish farms. Veterinary Parasitology 169, 391–394.Google Scholar
Aunpromma, S., Tangkawattana, P., Papirom, P., Kanjampa, P., Tesana, S., Sripa, B. & Tangkawattana, S. (2012) High prevalence of Opisthorchis viverrini infection in reservoir hosts in four districts of Khon Kaen Province, an opisthorchiasis endemic area of Thailand. Parasitology International 61, 60–64.Google Scholar
Bilqees, F.M., Shabbir, I. & Parveen, S. (2003) Neometorchis lobatum gen. n. sp. n. (Trematoda: Opisthorchiidae, Metorchinea) from the bird Anas sp. from Kalri Lake, Sindh. Proceedings of Parasitology 36, 7–12.Google Scholar
Bowles, J., Hope, M., Tiu, W.U., Liu, X. & McManus, D.P. (1993) Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum. Acta Tropica 55, 217–229.Google Scholar
Bray, R.A., Gibson, D.I. & Jones, A. (2008) Keys to the Trematoda, Volume 3. 848 pp. Wallingford, CABI and New York, Oxford University Press.CrossRefGoogle Scholar
Chai, J., Murrell, K.D. & Lymbery, A. (2005) Fish-borne parasitic zoonoses: status and issues. International Journal for Parasitology 35, 1233–1254.CrossRefGoogle ScholarPubMed
De, N.V. & Le, T.H. (2011) Human infections of fish-borne trematodes in Vietnam: prevalence and molecular specific identification at an endemic commune in Nam Dinh province. Experimental Parasitology 129, 355–361.Google Scholar
Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for window 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Kaewkes, S. (2003) Taxonomy and biology of liver flukes. Acta Tropica 88, 177–186.Google Scholar
Katoh, K. & Standley, D.M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: improvements in performance and usability. Molecular Biology and Evolution 30, 772–780.Google Scholar
Keiser, J. & Utzinger, J. (2005) Emerging foodborne trematodiasis. Emerging Infectious Diseases 11, 1507–1514.Google Scholar
Laoprom, N., Saijuntha, W., Sithithaworn, P., Wongkham, S., Laha, T., Ando, K., Andrews, R.H. & Petney, T.N. (2009) Biological variation within Opisthorchis viverrini sensu lato in Thailand and Lao PDR. Journal of Parasitology 95, 1307–1313.CrossRefGoogle ScholarPubMed
Le, N.T. (2000) Fauna of Vietnam. pp. 236–246. Hanoi, Science and Techniques Publishing House.Google Scholar
Mas-Coma, S. & Bargues, M.D. (1997) Human liver flukes: a review. Research and Reviews in Parasitology 57, 145–218.Google Scholar
Mordvinov, V.A., Yurlova, N.I., Ogorodova, L.M. & Katokhin, A.V. (2012) Opisthorchis felineus and Metorchis bilis are the main agents of liver fluke infection of humans in Russia. Parasitology International 61, 25–31.Google Scholar
Rice, P., Longden, I. & Bleasby, A. (2000) EMBOSS: the European Molecular Biology Open Software Suite. Trends in Genetics 16, 276–277.Google Scholar
Sithithaworn, P., Nuchjungreed, C., Srisawangwong, T., Ando, K., Petney, T.N., Chilton, N.B. & Andrews, R.H. (2007) Genetic variation in Opisthorchis viverrini (Trematoda: Opisthorchiidae) from northeast Thailand and Laos PDR based on random amplified polymorphic DNA analyses. Parasitology Research 100, 613–617.Google Scholar
Sohn, W.M., Shin, E.H., Yong, T.S., Eom, K.S., Jeong, H.G., Sinuon, M., Socheat, D. & Chai, J.Y. (2011) Adult Opisthorchis viverrini flukes in humans, Takeo, Cambodia. Emerging Infectious Diseases 17, 1302–1304.Google Scholar
Sripa, B., Brindley, P.J., Mulvenna, J., Laha, T., Smout, M.J., Mairiang, E., Bethony, J.M. & Loukas, A. (2012) The tumorigenic liver fluke Opisthorchis viverrini – multiple pathways to cancer. Trends in Parasitology 28, 395–407.Google Scholar
Thaenkham, U., Visetsuk, K., Dung, D.T. & Waikagul, J. (2007) Discrimination of Opisthorchis viverrini from Haplorchis taichui using COI sequence marker. Acta Tropica 103, 26–32.Google Scholar
Thaenkham, U., Nuamtanong, S., Vonghachack, Y., Yoonuan, T., Sanguankiat, S., Dekumyoy, P., Prommasack, B., Kobayashi, J. & Waikagul, J. (2011) Discovery of Opisthorchis lobatus (Trematoda: Opisthorchiidae): a new record of small liver flukes in the Greater Mekong Sub-region. Journal of Parasitology 97, 1152–1158.Google Scholar
Valero, M.A., Darce, N.A., Panova, M. & Mas-Coma, S. (2001) Relationships between host species and morphometric patterns in Fasciola hepatica adults and eggs from the northern Bolivian Altiplano hyperendemic region. Veterinary Parasitology 102, 85–100.Google Scholar
WHO (2011) A review of human carcinogens part B: biological agents. Opisthorchis viverrini and Clonorchis sinensis. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 100 B, 341–370.Google Scholar