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RETRACTED – Immune recognition of excretory and secretory products of the filarial nematode Onchocerca ochengi in cattle and human sera

Published online by Cambridge University Press:  11 September 2015

B. Djafsia ,
D. Ndjonka ,
J.V. Dikti ,
S. van Hoorn ,
K. Manchang ,
N. Brattig  and
E. Liebau
B. Djafsia
Affiliation:
Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, PO Box 454 Ngaoundéré, Cameroon Tropical Medicine Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany
D. Ndjonka*
Affiliation:
Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, PO Box 454 Ngaoundéré, Cameroon
J.V. Dikti
Affiliation:
Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, PO Box 454 Ngaoundéré, Cameroon Department of Molecular Physiology, Institute for Zoophysiology, Schlossplatz 8, 48143 Muenster, Germany
S. van Hoorn
Affiliation:
Tropical Medicine Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany
K. Manchang
Affiliation:
Institute of Agricultural Research for Development, Veterinary Research Laboratory, Wakwa regional centre, PO Box 65 Ngaoundéré, Cameroon
N. Brattig
Affiliation:
Tropical Medicine Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany
E. Liebau
Affiliation:
Department of Molecular Physiology, Institute for Zoophysiology, Schlossplatz 8, 48143 Muenster, Germany
*
* E-mail: dede_ndjonka@yahoo.com
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Abstract

Excretory–secretory (ES) products of nematodes and other helminths are the first molecules to interact with cell surfaces and soluble proteins within the host. In the present study, ES products of the filarial nematode Onchocerca ochengi were investigated as a model for Onchocerca volvulus, the causative agent of river blindness. These products were collected from adult and larval stages in vitro over a period of 7 days, to compare their immunological recognition in cattle and human sera, infected with species of Onchocerca. From the 156 sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) ES products or extracts, protein bands showed different patterns between female and male products. A comparison of antibody recognition of the different ES products by sera from infected cattle and humans, when analysed by enzyme-linked immunosorbent assay (ELISA), revealed a relatively higher reactivity of the female somatic extract to human and cattle sera compared to ES products of both genders. Nevertheless, similar reactivity of the O. ochengi male and female ES products to human and cattle sera was noticed. As a result, the interaction of ES products with the surface of the host and immune system often led to host responses, including the generation of antibodies. The O. ochengi ES products are therefore good sources of potential immunogenic proteins. The identification of these ES products is in progress, with the aim of developing vaccine candidates against human onchocerciasis.

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Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e90
Copyright
Copyright © Cambridge University Press 2015 

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