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Residual photosensitivity in mice lacking both rod opsin and cone photoreceptor cyclic nucleotide gated channel 3 α subunit

Published online by Cambridge University Press:  01 September 2004

ALUN R. BARNARD ,
JOANNE M. APPLEFORD ,
SUMATHI SEKARAN ,
KRISHNA CHINTHAPALLI ,
AARON JENKINS ,
MATHEAS SEELIGER ,
MARTIN BIEL ,
PETER HUMPHRIES ,
RON H. DOUGLAS  and
ANDREAS WENZEL
...Show all authors
ALUN R. BARNARD
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK Faculty of Life Sciences, University of Manchester, Manchester, UK
JOANNE M. APPLEFORD
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK
SUMATHI SEKARAN
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK
KRISHNA CHINTHAPALLI
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK
AARON JENKINS
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK
MATHEAS SEELIGER
Affiliation:
Retinal Electrodiagnostics Research Group, University Eye Hospital Tübingen, Department of Pathophysiology of Vision and Neuroophthalmology, Tübingen, Germany
MARTIN BIEL
Affiliation:
Lehrstuhl Pharmakologie für Naturwissenshcaften, Zentrum für Pharmaforschung, Ludwig-Maximilians Universität München, München, Germany
PETER HUMPHRIES
Affiliation:
Department of Genetics, Trinity College Dublin, Republic of Ireland
RON H. DOUGLAS
Affiliation:
Department of Optometry and Visual Science, City University, Northampton Square, London, UK
ANDREAS WENZEL
Affiliation:
Laboratory for Retinal Cell Biology, ONO-EM, H-Lab-13, Zürich, Switzerland
RUSSELL G. FOSTER
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK
MARK W. HANKINS
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK
ROBERT J. LUCAS
Affiliation:
Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, London, UK Faculty of Life Sciences, University of Manchester, Manchester, UK
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Abstract

The mammalian retina contains three classes of photoreceptor. In addition to the rods and cones, a subset of retinal ganglion cells that express the putative sensory photopigment melanopsin are intrinsically photosensitive. Functional and anatomical studies suggest that these inner retinal photoreceptors provide light information for a number of non-image-forming light responses including photoentrainment of the circadian clock and the pupil light reflex. Here, we employ a newly developed mouse model bearing lesions of both rod and cone phototransduction cascades (Rho−/−Cnga3−/−) to further examine the function of these non-rod non-cone photoreceptors. Calcium imaging confirms the presence of inner retinal photoreceptors in Rho−/−Cnga3−/− mice. Moreover, these animals retain a pupil light reflex, photoentrainment, and light induction of the immediate early gene c-fos in the suprachiasmatic nuclei, consistent with previous findings that pupillary and circadian responses can employ inner retinal photoreceptors. Rho−/−Cnga3−/− mice also show a light-dependent increase in the number of FOS-positive cells in both the ganglion cell and (particularly) inner nuclear layers of the retina. The average number of cells affected is several times greater than the number of melanopsin-positive cells in the mouse retina, suggesting functional intercellular connections from these inner retinal photoreceptors within the retina. Finally, however, while we show that wild types exhibit an increase in heart rate upon light exposure, this response is absent in Rho−/−Cnga3−/− mice. Thus, it seems that non-rod non-cone photoreceptors can drive many, but not all, non-image-forming light responses.

Keywords

MelanopsinCircadian entrainmentRetinal degenerationHeart rateLight

Information

Type
Research Article
Information
Visual Neuroscience , Volume 21 , Issue 5 , September 2004 , pp. 675 - 683
Copyright
2004 Cambridge University Press

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