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DNA ligase activity in deteriorated maize embryo axes during germination: a model relating defects in DNA metabolism in seeds to loss of germinability

Published online by Cambridge University Press:  19 September 2008

Edgar Vázquez ,
Fernando Montiel  and
Jorge M. Vázquez-Ramos
Edgar Vázquez
Affiliation:
Depto. de Bioquímica Vegetal, División de Bioquímica y Farmacia, Facultad de Química, UNAM. Avenida Universidad y Copilco. México 04510, D.F.México
Fernando Montiel
Affiliation:
Depto. de Bioquímica Vegetal, División de Bioquímica y Farmacia, Facultad de Química, UNAM. Avenida Universidad y Copilco. México 04510, D.F.México
Jorge M. Vázquez-Ramos*
Affiliation:
Depto. de Bioquímica Vegetal, División de Bioquímica y Farmacia, Facultad de Química, UNAM. Avenida Universidad y Copilco. México 04510, D.F.México
*
* Correspondence
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Abstract

Chromosome integrity and DNA polymerase activity are negatively affected by improper storage of maize seeds. We have extended our studies on the effect of improper storage on DNA metabolism by analysing the effect of hot–dry and hot–humid conditions on DNA ligase activity. An assay to measure ligase activity was established by using linearized plasmid DNA as substrate and protein extracts from maize axes. Optimal conditions were established for the in vitro assay. Enzyme activity was importantly reduced under both storage conditions. Activity recovered 6–9 h after imbibition. A model for loss of viability and vigour due to damage of DNA metabolism is proposed.

Keywords

DNA ligasegerminationmaizeseed deteriorationZea mays
Type
Research Papers
Information
Seed Science Research , Volume 1 , Issue 4 , December 1991 , pp. 269 - 273
Copyright
Copyright © Cambridge University Press 1991

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