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Dynamics of the contents and distribution of ABA, auxins and aquaporins in developing caryopses of an ABA-deficient barley mutant and its parental cultivar

Published online by Cambridge University Press:  11 December 2019

Oksana A. Seldimirova Open the ORCID record for Oksana A. Seldimirova [Opens in a new window] ,
Guzel R. Kudoyarova Open the ORCID record for Guzel R. Kudoyarova [Opens in a new window] ,
Maki Katsuhara ,
Ilshat R. Galin ,
Denis Yu. Zaitsev ,
Natalia N. Kruglova Open the ORCID record for Natalia N. Kruglova [Opens in a new window] ,
Dmitry S. Veselov  and
Stanislav Yu. Veselov
Oksana A. Seldimirova*
Affiliation:
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa450054, Russia
Guzel R. Kudoyarova
Affiliation:
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa450054, Russia
Maki Katsuhara
Affiliation:
Okayama University, Institute of Plant Science and Resources, Kurashiki, Okayama710-0046, Japan
Ilshat R. Galin
Affiliation:
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa450054, Russia
Denis Yu. Zaitsev
Affiliation:
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa450054, Russia
Natalia N. Kruglova
Affiliation:
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa450054, Russia
Dmitry S. Veselov
Affiliation:
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa450054, Russia
Stanislav Yu. Veselov
Affiliation:
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa450054, Russia Department of Biochemistry and Biotechnology, Faculty of Biology, Bashkir State University, Zaki Validi Street 32, Ufa450076, Russia
*
Author for correspondence: Oksana A. Seldimirova, Email: o_seldimirova@mail.ru
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Abstract

Dynamics of abscisic acid (ABA) and indole-3-acetic acid (IAA) contents were followed in developing barley caryopses of the ABA-deficient mutant AZ34 and its parental cultivar Steptoe. Distribution of these hormones and HvPIP2 aquaporins (AQPs) was studied with the help of immunohistochemical methods in the roots and coleorhiza of developing embryos. In Steptoe, maturation of the caryopsis was accompanied by vast accumulation of ABA, while this hormone accumulated more slowly in the caryopsis of AZ34 and its content was lower than in Steptoe. Accumulation of ABA was accompanied by a decline in IAA level in the developing caryopsis, the process being delayed in AZ34 in accordance with the slower accumulation of ABA. ABA accumulated to high levels in the coleorhiza cells of Steptoe, while the effect was absent in AZ34. The high level of ABA was likely to be important for maintaining the barrier function of the coleorhiza, preventing germination of seminal roots and enabling seed dormancy, while the absence of ABA accumulation in coleorhiza of AZ34 may be responsible for the initiation of root germination inside the caryopsis. The abundance of HvPIP2 AQPs in the seminal roots was higher at the beginning of maturation of Steptoe caryopsis and declined afterwards, while the levels of APQs increased later in AZ34 in accordance with the delay in ABA accumulation. These results suggest the importance of ABA accumulation in coleorhiza for preventing precocious growth of seminal roots, and suggest regulation of IAA and aquaporin levels by this hormone during maturation of embryos.

Keywords

abscisic acidaquaporinauxinbarleycaryopsis developmentembryo formation

Information

Type
Research Paper
Information
Seed Science Research , Volume 29 , Issue 4 , December 2019 , pp. 261 - 269
Copyright
Copyright © Cambridge University Press 2019

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