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Effects of sorbitol on porcine oocyte maturation and embryo development in vitro

Published online by Cambridge University Press:  02 January 2014

Tao Lin ,
Jin Yu Zhang ,
Yun Fei Diao ,
Jung Won Kang  and
Dong-Il Jin
Tao Lin
Affiliation:
Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305–764, Republic of Korea
Jin Yu Zhang
Affiliation:
Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305–764, Republic of Korea
Yun Fei Diao
Affiliation:
Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305–764, Republic of Korea
Jung Won Kang
Affiliation:
Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305–764, Republic of Korea
Dong-Il Jin*
Affiliation:
Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305–764, Republic of Korea Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305–764, Republic of Korea
*
All correspondence to: Dong-Il Jin. Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305–764, Republic of Korea. e-mail: dijin@cnu.ac.kr
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Summary

In the present study, a porcine system was supplemented with sorbitol during in vitro maturation (IVM) or in vitro culture (IVC), and the effects of sorbitol on oocyte maturation and embryonic development following parthenogenetic activation were assessed. Porcine immature oocytes were treated with different concentrations of sorbitol during IVM, and the resultant metaphase II stage oocytes were activated and cultured in porcine zygote medium-3 (PZM-3) for 7 days. No significant difference was observed in cumulus expansion and the nuclear maturation between the control and sorbitol-treated groups, with the exception of the 100 mM group, which showed significantly decreased nuclear maturation and cumulus expansion. There was no significant difference in the intracellular reactive oxygen species (ROS) levels between oocytes matured with 10 or 20 mM sorbitol and control groups, but 50 and 100 mM groups had significantly higher ROS levels than other groups. The 20 mM group showed significant increases in intracellular glutathione and subsequent blastocyst formation rates following parthenogenetic activation compared with the other groups. During IVC, supplementation with sorbitol significantly reduced blastocyst formation and increased the apoptotic index compared with the control. The apoptotic index of blastocysts from the sorbitol-treated group for entire culture period was significantly higher than those of the partially sorbitol-exposed groups. Based on these findings, it can be concluded that the addition of a low concentration of sorbitol (20 mM) during IVM of porcine oocytes benefits subsequent blastocyst development and improves embryo quality, whereas sorbitol supplement during IVC has a negative effect on blastocyst formation.

Keywords

ApoptosisEmbryonic developmentOocyte maturationPorcine oocyteSorbitol
Type
Research Article
Information
Zygote , Volume 23 , Issue 2 , April 2015 , pp. 297 - 306
Copyright
Copyright © Cambridge University Press 2014 

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