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Fresh oocyte cycles yield improved embryo quality compared with frozen oocyte cycles in an egg-sharing donation programme

Published online by Cambridge University Press:  18 January 2021

Amanda Souza Setti Open the ORCID record for Amanda Souza Setti [Opens in a new window] ,
Daniela Paes de Almeida Ferreira Braga ,
Assumpto Iaconelli Jr  and
Edson Borges Jr
Amanda Souza Setti*
Affiliation:
Fertility Medical Group, Av. Brigadeiro Luis Antonio, 4545, São Paulo, SP 01401-002, Brazil Sapientiae Institute – Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62 São Paulo, SP 04503-040, Brazil
Daniela Paes de Almeida Ferreira Braga
Affiliation:
Fertility Medical Group, Av. Brigadeiro Luis Antonio, 4545, São Paulo, SP 01401-002, Brazil Sapientiae Institute – Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62 São Paulo, SP 04503-040, Brazil
Assumpto Iaconelli Jr
Affiliation:
Fertility Medical Group, Av. Brigadeiro Luis Antonio, 4545, São Paulo, SP 01401-002, Brazil Sapientiae Institute – Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62 São Paulo, SP 04503-040, Brazil
Edson Borges Jr
Affiliation:
Fertility Medical Group, Av. Brigadeiro Luis Antonio, 4545, São Paulo, SP 01401-002, Brazil Sapientiae Institute – Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62 São Paulo, SP 04503-040, Brazil
*
Author for correspondence: Amanda Setti. Av. Brigadeiro Luis Antonio, 4545, São Paulo, SP 01401-002, Brazil. Tel: +55 11 3018 8181. E-mail: amanda@sapientiae.org.br
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Summary

The objective of this study was to investigate any effect of cryopreservation of donated eggs on laboratorial and clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles. This retrospective cohort study included 320 oocyte recipients undergoing 307 vitrified and 119 fresh oocyte recipient ICSI cycles, participating in an egg-sharing donation programme, from 2015 to 2018, in a private university-affiliated in vitro fertilization (IVF) centre. A review of donor and recipient ICSI cycles was charted. A general mixed models fit by restricted maximum likelihood, followed by Bonferroni post hoc test was used to compare the means between fresh and warm oocyte donation groups and investigate the effect of cryopreservation on recipient ICSI outcome. The main outcome measure was blastocyst development rates. Fertilization rate, high-quality embryo rates on days 2 and 3, normal cleavage speed rates on days 2 and 3, and blastocyst development rate were significantly higher for the fresh oocyte donation cycles compared with warmed oocyte donation cycles. In the egg-sharing donation programme, fertilization and embryo developmental competence were reduced when vitrified oocytes from infertile couples were used for ICSI compared with fresh oocytes.

Keywords

CryopreservationEmbryoICSIOocyteVitrification

Information

Type
Research Article
Information
Zygote , Volume 29 , Issue 3 , June 2021 , pp. 234 - 238
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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References

Boiso, I, Veiga, A and Edwards, RG (2002). Fundamentals of human embryonic growth in vitro and the selection of high-quality embryos for transfer. Reprod Biomed Online 5, 328–50.CrossRefGoogle ScholarPubMed
Braga, DP, Setti, AS, Figueira, RC, Azevedo Mde, C, Iaconelli, A Jr, Lo Turco, EG and Borges, E Jr (2016). Freeze-All, oocyte vitrification, or fresh embryo transfer? Lessons from an egg-sharing donation program. Fertil Steril 106, 615–22.CrossRefGoogle ScholarPubMed
Chamayou, S, Alecci, C, Ragolia, C, Storaci, G, Maglia, E, Russo, E and Guglielmino, A (2006). Comparison of in-vitro outcomes from cryopreserved oocytes and sibling fresh oocytes. Reprod Biomed Online 12, 730–6.CrossRefGoogle ScholarPubMed
Chen, C (1986). Pregnancy after human oocyte cryopreservation. Lancet 1, 884–6.CrossRefGoogle ScholarPubMed
Chen, SU, Lien, YR, Chang, LJ, Tsai, YY, Ho, HN and Yang, YS (2004). Cryopreserved sibling oocytes and intracytoplasmic sperm injection rescue unexpectedly poor fertilization in conventional in vitro fertilization. J Assist Reprod Genet 21, 367–9.CrossRefGoogle ScholarPubMed
Cobo, A, Kuwayama, M, Pérez, S, Ruiz, A, Pellicer, A and Remohí, J (2008). Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril 89, 1657–64.CrossRefGoogle ScholarPubMed
Cobo, A, Coello, A, Remohí, J, Serrano, J, de Los Santos, JM and Meseguer, M (2017). Effect of oocyte vitrification on embryo quality: time-lapse analysis and morphokinetic evaluation. Fertil Steril 108, 491–7.e493.CrossRefGoogle ScholarPubMed
Coello, A, Pellicer, A and Cobo, A (2018). Vitrification of human oocytes. Minerva Ginecol 70, 415–23.Google ScholarPubMed
Forman, EJ, Li, X, Ferry, KM, Scott, K, Treff, NR and Scott, RT Jr (2012). Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting. Fertil Steril 98, 644–9.CrossRefGoogle ScholarPubMed
García, JI, Noriega-Portella, L and Noriega-Hoces, L (2011). Efficacy of oocyte vitrification combined with blastocyst stage transfer in an egg donation program. Hum Reprod 26, 782–90.CrossRefGoogle Scholar
Goldman, KN, Noyes, NL, Knopman, JM, McCaffrey, C and Grifo, JA (2013). Oocyte efficiency: does live birth rate differ when analyzing cryopreserved and fresh oocytes on a per-oocyte basis? Fertil Steril 100, 712–7.CrossRefGoogle ScholarPubMed
Hara, H, Hwang, IS, Kagawa, N, Kuwayama, M, Hirabayashi, M and Hochi, S (2012). High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization. Theriogenology 77, 908–15.CrossRefGoogle ScholarPubMed
Iussig, B, Maggiulli, R, Fabozzi, G, Bertelle, S, Vaiarelli, A, Cimadomo, D, Ubaldi, FM and Rienzi, L (2019). A brief history of oocyte cryopreservation: arguments and facts. Acta Obstet Gynecol Scand 98, 550–8.CrossRefGoogle ScholarPubMed
Jones, A, Van Blerkom, J, Davis, P and Toledo, AA (2004). Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence. Hum Reprod 19, 1861–6.CrossRefGoogle ScholarPubMed
Kuwayama, M, Vajta, G, Kato, O and Leibo, SP (2005). Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online 11, 300–8.CrossRefGoogle ScholarPubMed
Magli, MC, Lappi, M, Ferraretti, AP, Capoti, A, Ruberti, A and Gianaroli, L (2010). Impact of oocyte cryopreservation on embryo development. Fertil Steril 93, 510–6.CrossRefGoogle ScholarPubMed
Moussa, M, Shu, J, Zhang, X and Zeng, F (2014). Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci 57, 903–14.CrossRefGoogle ScholarPubMed
Nohales-Córcoles, M, Sevillano-Almerich, G, Di Emidio, G, Tatone, C, Cobo, AC, Dumollard, R and De Los Santos Molina, MJ (2016). Impact of vitrification on the mitochondrial activity and redox homeostasis of human oocyte. Hum Reprod 31, 1850–8.CrossRefGoogle ScholarPubMed
Parmegiani, L, Cognigni, GE, Bernardi, S, Cuomo, S, Ciampaglia, W, Infante, FE, Tabarelli de Fatis, C, Arnone, A, Maccarini, AM and Filicori, M (2011). Efficiency of aseptic open vitrification and hermetical cryostorage of human oocytes. Reprod Biomed Online 23, 505–12.CrossRefGoogle ScholarPubMed
Pickering, SJ, Braude, PR, Johnson, MH, Cant, A and Currie, J (1990). Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Steril 54, 102–8.CrossRefGoogle ScholarPubMed
Rienzi, L, Martínez, F, Ubaldi, F, Minasi, MG, Iacobelli, M, Tesarik, J and Greco, E (2004). Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures. Hum Reprod 19, 655–9.CrossRefGoogle ScholarPubMed
Rienzi, L, Romano, S, Albricci, L, Maggiulli, R, Capalbo, A, Baroni, E, Colamaria, S, Sapienza, F and Ubaldi, F (2010). Embryo development of fresh ‘versus’ vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study. Hum Reprod 25, 66–73.CrossRefGoogle ScholarPubMed
Siano, L, Engmann, L, Nulsen, J and Benadiva, C (2013). A prospective pilot study comparing fertilization and embryo development between fresh and vitrified sibling oocytes. Conn Med 77, 211–7.Google ScholarPubMed
Solé, M, Santaló, J, Boada, M, Clua, E, Rodríguez, I, Martínez, F, Coroleu, B, Barri, PN and Veiga, A (2013). How does vitrification affect oocyte viability in oocyte donation cycles? A prospective study to compare outcomes achieved with fresh versus vitrified sibling oocytes. Hum Reprod 28, 2087–92.CrossRefGoogle ScholarPubMed
Trokoudes, KM, Pavlides, C and Zhang, X (2011). Comparison outcome of fresh and vitrified donor oocytes in an egg-sharing donation program. Fertil Steril 95, 1996–2000.CrossRefGoogle Scholar
Van Blerkom, J, Davis, P and Alexander, S (2000). Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence. Hum Reprod 15, 2621–33.CrossRefGoogle ScholarPubMed