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Nuclear-to-cytoplasmic ratios of 1PN and 2PN zygotes after in vitro fertilization of mouse oocytes

Published online by Cambridge University Press:  28 June 2021

Natsumi Okajima ,
Wei Xiao ,
Alex Lopata ,
Tadashi Sankai ,
Lubna Yasmin ,
Yasushi Nagai ,
Ryota Okamoto ,
Hidetaka Tasaki Open the ORCID record for Hidetaka Tasaki [Opens in a new window]  and
Junko Otsuki Open the ORCID record for Junko Otsuki [Opens in a new window]
Natsumi Okajima
Affiliation:
Department of Animal Science, Okayama University, Okayama, Japan
Wei Xiao
Affiliation:
Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan
Alex Lopata
Affiliation:
Retired, previously worked at the Department of Obstetrics and Gynecology, Royal Women’s Hospital, University of Melbourne, Australia
Tadashi Sankai
Affiliation:
Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Japan
Lubna Yasmin
Affiliation:
Department of Oncology, Cross Cancer Institute, University of Alberta, Alberta, Canada
Yasushi Nagai
Affiliation:
Nagai Mother’s Hospital, Saitama, Japan
Ryota Okamoto
Affiliation:
Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
Hidetaka Tasaki
Affiliation:
Department of Animal Science, Okayama University, Okayama, Japan Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan Assisted Reproductive Technology Center, Okayama University, Okayama, Japan
Junko Otsuki*
Affiliation:
Department of Animal Science, Okayama University, Okayama, Japan Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan Assisted Reproductive Technology Center, Okayama University, Okayama, Japan
*
Author for correspondence: J. Otsuki. Department of Animal Science, Okayama University, Okayama, Japan. Email: otsuki.midori.junko@gmail.com
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Summary

Numerous studies have reported comparisons of the nuclear-to-cytoplasmic (NC) ratio during mitosis. However, little information is known about how the pronuclear size is regulated and determined at the end of meiosis II in mammalian zygotes. The present study aims to analyze the NC ratio of female and male pronuclei, and also to compare the size of single pronuclei using photographs that were obtained during experiments to create chimeric hermaphrodites from 2-cell oocytes. The volume of both the female and the male pronucleus was found to correlate with the volume of the oocyte cytoplasm. The NC ratio of the male pronucleus was greater than that of the female pronucleus. The NC ratio of the average volume of the female and male pronuclei was greater than the NC ratio of the mononucleate oocytes. The occurrence of 1PN oocytes was significantly higher when the volume of cytoplasm was lower than the cut-off value. These results indicated that the NC ratio is retained during pronuclear formation. A higher NC ratio in male compared with the female pronucleus indicated structural and/or molecular difference between the two pronuclei. 1PN formation may occur when sperm enters close to the MII spindle.

Keywords

Pronucleuscell volumenucleus-cell ratiomeiosis1PN
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 120 - 124
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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