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The effect of Coenzyme Q10 on mitochondrial biogenesis in mouse ovarian follicles during in vitro culture

Published online by Cambridge University Press:  04 December 2023

Roya Harsini ,
Saeed Zavareh Open the ORCID record for Saeed Zavareh [Opens in a new window] ,
Meysam Nasiri  and
Sara Seyfi
Roya Harsini
Affiliation:
School of Biology, Damghan University, Damghan, Iran
Saeed Zavareh*
Affiliation:
School of Biology, Damghan University, Damghan, Iran Institute of Biological Sciences, Damghan University, Damghan, Iran
Meysam Nasiri
Affiliation:
School of Biology, Damghan University, Damghan, Iran Institute of Biological Sciences, Damghan University, Damghan, Iran
Sara Seyfi
Affiliation:
School of Biology, Damghan University, Damghan, Iran
*
Corresponding author: Saeed Zavareh; Email: zavareh.s@du.ac.ir
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Summary

The aim of this research was to investigate the effect of Coenzyme Q10 (CoQ10) on the expression of the Transcription Factor A Mitochondrial (Tfam) gene and mtDNA copy number in preantral follicles (PFs) of mice during in vitro culture. To conduct this experimental study, PFs were isolated from 14-day-old National Medical Research Institute mice and cultured in the presence of 50 µm CoQ10 for 12 days. On the 12th day, human chorionic gonadotropin was added to stimulate ovulation. The fundamental parameters, including preantral follicle developmental rate and oocyte maturation, were evaluated. Additionally, the Tfam gene expression and mtDNA copy number of granulosa cells and oocytes were assessed using the real-time polymerase chain reaction. The results revealed that CoQ10 significantly increased the diameter of PFs, survival rate, antrum formation, and metaphase II (MII) oocytes (P < 0.05). Moreover, in the CoQ10-treated groups, the Tfam gene expression in granulosa cells and oocytes increased considerably compared with the control group. The mtDNA copy number of granulosa cells and oocytes cultured in the presence of CoQ10 was substantially higher compared with the control groups (P < 0.05). The addition of CoQ10 to the culture medium enhances the developmental competence of PFs during in vitro culture by upregulating Tfam gene expression and increasing mtDNA copy number in oocyte and granulosa cells.

Keywords

Coenzyme Q10mtDNAOvaryPreantral follicleTfam

Information

Type
Research Article
Information
Zygote , Volume 32 , Issue 1 , February 2024 , pp. 14 - 20
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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