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SCAR molecular markers of the B biotype and two non-B populations of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

Published online by Cambridge University Press:  20 March 2007

Zang Lian-Sheng ,
Jiang Tong ,
Xu Jing ,
Liu Shu-Sheng  and
Zhang You-Jun
Zang Lian-Sheng
Affiliation:
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China College of Agronomy, Jilin Agricultural University, Changchun 130118, China
Jiang Tong
Affiliation:
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Xu Jing
Affiliation:
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
Liu Shu-Sheng*
Affiliation:
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
Zhang You-Jun
Affiliation:
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
*Corresponding author. E-mail: shshliu@zju.edu.cn
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Abstract

Random amplified polymorphic DNA (RAPD) analyses were performed with random primer H16 for the B biotype and two non-B populations of Bemisia tabaci collected from Zhejiang (China). The specific sequence fragments containing 446, 390 and 1317 nucleotides were amplified for the B biotype, ZHJ-1, ZHJ-2 populations, respectively. The three specific fragments were cloned and sequenced, and three pairs of SCAR primers were designed according to the sequences determined. With improvement of the conditions of the polymerase chain reaction (PCR), the specific fragments of B biotype, ZHJ-1 and ZHJ-2 populations, namely 439, 366 and 1238 nucleotides, respectively, were amplified with the sequence characterized amplified region (SCAR) primer of the corresponding population, while specific fragments of the other populations of B. tabaci or Trialeurodes vaporariorum could not be amplified.

Keywords

Bemisia tabacibiotypemolecular markerspopulationRAPDSCAR

Information

Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 3 , December 2006 , pp. 189 - 194
Copyright
China Agricultural University and Cambridge University Press 2006

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