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Genetic diversity among potato species as revealed by phenotypic resistances and SSR markers

Published online by Cambridge University Press:  03 January 2013

D. Carputo ,
D. Alioto ,
R. Aversano ,
R. Garramone ,
V. Miraglia ,
C. Villano  and
L. Frusciante
D. Carputo*
Affiliation:
Department of Soil Plant, Environmental, and Animal Production Sciences, University of Naples Federico II, Via Università 100, 80055Portici, Italy
D. Alioto
Affiliation:
Department of Arboriculture, Botany and Plant Pathology, University of Naples Federico II, Via Università 100, 80055, Portici, Italy
R. Aversano
Affiliation:
Department of Soil Plant, Environmental, and Animal Production Sciences, University of Naples Federico II, Via Università 100, 80055Portici, Italy
R. Garramone
Affiliation:
Department of Soil Plant, Environmental, and Animal Production Sciences, University of Naples Federico II, Via Università 100, 80055Portici, Italy
V. Miraglia
Affiliation:
Department of Soil Plant, Environmental, and Animal Production Sciences, University of Naples Federico II, Via Università 100, 80055Portici, Italy
C. Villano
Affiliation:
Department of Soil Plant, Environmental, and Animal Production Sciences, University of Naples Federico II, Via Università 100, 80055Portici, Italy
L. Frusciante
Affiliation:
Department of Soil Plant, Environmental, and Animal Production Sciences, University of Naples Federico II, Via Università 100, 80055Portici, Italy
*
*Corresponding author. E-mail: carputo@unina.it
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Abstract

The evolutionary diversity of wild potato species makes them excellent materials for improving the narrow genetic basis of the cultivated potato Solanum tuberosum. Understanding their genetic diversity is important not only to choose the best parents for breeding, but also to design proper crossing schemes and selection strategies. The objectives of this study were to determine the resistance response to Ralstonia solanacearum, Potato virus Y and low temperatures of 21 clones of 12 potato species, and to determine their genetic diversity through simple sequence repeat (SSR) markers. Sources of resistance have been found for all the investigated traits, with high resistance variability not only between but also within species. Combined resistances were also identified, with positive implications for efficient breeding. SSR analysis allowed the detection of 12 loci and 46 alleles across all genotypes, with an average value of 3.8 alleles per locus. Both unique and rare alleles useful for marker-assisted selection were found. SSR-based cluster analysis revealed that resistant genotypes were distributed among all clusters, suggesting that genetically different resistant genotypes were identified. The information obtained in this study is discussed from a breeding perspective.

Keywords

low temperaturesmarker-assisted selectionPVYRalstonia solanacearumSolanum spp

Information

Type
Research Article
Information
Plant Genetic Resources , Volume 11 , Issue 2 , August 2013 , pp. 131 - 139
Copyright
Copyright © NIAB 2013 

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References

Aversano, R, Ercolano, MR, Frusciante, L, Monti, L, Bradeen, JM, Cristinzio, G, Zoina, A, Greco, N, Vitale, S and Carputo, D (2007) Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes. Genetic Resources and Crop Evolution 27: 1797–1806.Google Scholar
Bisognin, DA and Douches, DS (2002) Genetic diversity in diploid and tetraploid potato late blight resistant germplasm. Horticultural Science 37: 178–183.Google Scholar
Bradeen, JM, Carputo, D and Douches, DS (2008) Potato. In: Kole, C and Hall, TC (eds) Compendium of Transgenic Crop Plants: Transgenic Sugar, Tuber and Fiber Crops. Oxford: Blackwell, pp. 117–156.CrossRefGoogle Scholar
Bradshaw, J (2007) Potato-breeding strategies. In: Vreugdenhil, D, Bradshaw, J, Gebhardt, C, Governs, F, Mackerron, DKL, Taylor, MA and Ross, HA (eds) Potato Biology and Biotechnology: Advances and Perspectives. Oxford: Elsevier, pp. 157–179.Google Scholar
Caicedo, AL and Schaal, BA (2004) Heterogeneous evolutionary processes affect R gene diversity in natural populations of Solanum pimpinellifolium. Proceedings of the National Academy of Sciences of the United States of America 101: 17444–17449.CrossRefGoogle ScholarPubMed
Camadro, EL, Erazzú, LE, Maune, JF and Bedogni, MC (2012) A genetic approach to the species problem in wild potato. Plant Biology 14: 543–554.Google Scholar
Carputo, D, Terra, A, Barone, A, Esposito, F, Fogliano, V, Monti, L and Frusciante, L (2003) Glycoalkaloids and acclimation capacity of hybrids between Solanum tuberosum and the incongruent hardy species S. commersonii. Theoretical and Applied Genetics 107: 1187–1194.Google Scholar
Carputo, D, Camadro, EL and Peloquin, SJ (2006) Terminology for polyploids based on cytogenetic behavior: consequences in genetics and breeding. Plant Breeding Reviews 26: 105–124.Google Scholar
Carputo, D, Aversano, R, Barone, A, Di Matteo, A, Iorizzo, M, Sigillo, L, Zoina, A and Frusciante, L (2009) Resistance to Ralstonia solanacearum of sexual hybrids between Solanum commersonii and S. tuberosum. American Journal of Potato Research 86: 196–202.Google Scholar
Dodig, D, Zorić, M, Kobiljski, B, Šurlan-Momirović, G and Quarrie, SA (2010) Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data. Crop and Pasture Science 61: 812–824.Google Scholar
Feingold, S, Lloyd, J, Norero, N, Bonierbale, M and Lorenzen, J (2005) Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.). Theoretical and Applied Genetics 111: 456–466.Google Scholar
Felsenstein, J (2004) PHYLIP (Phylogeny Inference Package) Version 3.6. Seattle, WA: University of Washington.Google Scholar
Fock, I, Collonnier, C, Luisetti, J, Purwito, A, Souvannavong, V, Vedel, F, Servaes, A, Ambroise, A, Kodja, H, Ducreux, G and Sihachakr, D (2001) Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato. Plant Physiology and Biochemistry 39: 899–908.Google Scholar
Garcia-Arenal, F, Fraile, A and Malpica, JM (2001) Variability and genetic structure of plant virus populations. Annual Review of Phytopathology 39: 157–186.Google Scholar
Garcia-Vallvé, S, Palau, J and Romeu, A (1999) Horizontal gene transfer in glycosyl hydrolases inferred from codon usage in Escherichia coli and Bacillus subtilis. Molecular Biology and Evolution 16: 1125–1134.Google Scholar
Gebhardt, C (2007) Molecular markers, maps and population genetics. In: Vreugdenhil, D, Bradshaw, J, Gebhardt, C, Governs, F, Mackerron, DKL, Taylor, MA and Ross, HA (eds) Potato Biology and Biotechnology: Advances and Perspectives. Oxford: Elsevier, pp. 77–86.Google Scholar
Gebhardt, C and Valkonen, JP (2001) Organization of genes controlling disease resistance in the potato genome. Annual Review of Phytopathology 39: 79–102.Google Scholar
Ghislain, M, Spooner, DM, Rodríguez, F, Villamón, F, Núñez, J, Vásquez, C, Waugh, R and Bonierbale, M (2004) Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theoretical and Applied Genetics 108: 881–890.CrossRefGoogle ScholarPubMed
Ghislain, M, Núñez, J, del Rosario Herrera, M, Pignataro, J, Guzman, F, Bonierbale, DM and Spooner, DM (2009) Robust and highly informative microsatellite-based genetic identity kit for potato. Molecular Breeding 23: 377–388.Google Scholar
Gower, JC (1971) A general coefficient of similarity and some of its properties. Biometrics 27: 857–871.Google Scholar
Janácek, J and Prásil, I (1991) Quantification of plant frost injury by nonlinear fitting of an S-shaped function. Cryo-Letters 12: 47–52.Google Scholar
Jansky, SH (2000) Breeding for disease resistance in potato. Plant Breeding Reviews 19: 69–155.Google Scholar
Jansky, SH (2006) Overcoming hybridization barriers in potato. Plant Breeding 125: 1–12.Google Scholar
Jansky, SH (2011) Parental effects on the performance of cultivated × wild species hybrids in potato. Euphytica 178: 273–281.Google Scholar
Kalia, RK, Rai, MK, Kalia, S, Singh, R and Dhawan, AK (2011) Microsatellite markers: an overview of the recent progress in plants. Euphytica 177: 309–334.Google Scholar
Kloosterman, AD, Budowle, B and Daselaar, P (1993) PCR-amplification and detection of the human D1S80 VNTR locus. Amplification conditions, population genetics and application in forensic analysis. International Journal of Legal Medicine 105: 257–264.CrossRefGoogle ScholarPubMed
Letunic, I and Bork, P (2011) Interactive Tree Of Life v2: online annotation and display of phylogenetic trees made easy. Nucleic Acids Research 39 (Web Server issue): W475–W478.Google Scholar
Llop, P, Caruso, P, Cubero, J, Morente, C and López, MM (1999) A simple extraction procedure for efficient routine detection of pathogenic bacteria in plant material by polymerase chain reaction. Journal of Microbiological Methods 37: 23–31.Google Scholar
Maccaferri, M, Stefanelli, S, Rotondo, F, Tuberosa, R and Sanguineti, MC (2007) Relationships among durum wheat accessions. I. Comparative analysis of SSR, AFLP and phenotypic data. Genome 50: 373–384.Google Scholar
Martinez, L, Cavagnaro, P, Masuelli, R and Rodríguez, J (2003) Evaluation of diversity among Argentine grapevine (Vitis vinifera L.) varieties using morphological data and AFLP markers. Electronic Journal of Biotechnology 6: 241–246.Google Scholar
Mauricio, R, Stahl, EA, Korves, T, Tian, D, Kreitman, M and Bergelson, J (2003) Natural selection for polymorphism in the disease resistance gene Rps2 of Arabidopsis thaliana. Genetics 163: 735–746.Google Scholar
Milbourne, D, Meyer, RC, Collins, AJ, Ramsay, LD, Gebhardt, C and Waugh, R (1998) Isolation, characterisation and mapping of simple sequence repeat loci in potato. Molecular and General Genetics 259: 233–245.Google Scholar
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.Google Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70: 3321–3323.Google Scholar
Ochoa, CM (1990) The Potatoes of South America: Bolivia. Cambridge, New York: Cambridge University Press, p. 512.Google Scholar
Palta, JP and Simon, G (1993) Breeding potential for improvement of freezing stress resistance: genetic separation of freezing tolerance, freezing avoidance, and capacity to cold acclimate. In: Li, PH and Christersson, L (eds) Advances in Plant Cold Hardiness. Boca Raton, FL: CRC Press, pp. 299–310.Google Scholar
Potato Genome Sequencing Consortium (2011) Genome sequence and analysis of the tuber crop potato. Nature 475: 189–195.Google Scholar
Rigotti, S and Gugerli, P (2007) Rapid identification of potato virus Y strains by one-step triplex RT-PCR. Journal of Virological Methods 140: 90–94.Google Scholar
Seal, SE, Jackson, LA, Young, JPW and Daniels, MJ (1993) Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii and the Blood Disease Bacterium by partial 16s rRNA sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. Journal of General Microbiology 139: 1587–1594.CrossRefGoogle ScholarPubMed
Shehzad, T, Okuizumi, H, Kawase, M and Okuno, K (2009) Development of SSR-based sorghum (Sorghum bicolor (L.) Moench) diversity research set of germplasm and its evaluation by morphological traits. Genetic Resources and Crop Evolution 56: 809–827.Google Scholar
Simko I, Jansky S, Stephenson S and Spooner D (2007) Genetics of resistance to pests and diseases. In: Vreugdenhil D, Bradshaw J, Gebhardt C, Governs F, Mackerron DKL, Taylor MA and Ross HA (eds) Potato Biology and Biotechnology: Advances and Perspectives. Oxford: Elsevier, pp. 117–155.Google Scholar
Singh, RP, Valkonen, JPT, Gray, SM, Boonham, N, Jones, RAC, Kerlan, C and Schubert, J (2008) Discussion paper: the naming of Potato virus Y strains infecting potato. Archives of Virology 153: 1–13.CrossRefGoogle ScholarPubMed
Smith, SM, Pryor, AJ and Hulbert, SH (2004) Allelic and haplotypic diversity at the Rp1 rust resistance locus of maize. Genetics 167: 1939–1947.Google Scholar
Sneath PH and Sokal RR (1973) Numerical Taxonomy. The Principle and Practice of Numerical Classification. San Francisco, CA: WH Freeman and Company.Google Scholar
Song, J, Bradeen, JM, Naess, SK, Raasch, JA, Wielgus, SM, Haberlach, GT, Liu, J, Kuang, H, Austin-Phillips, S, Buell, CR, Helgeson, JP and Jiang, J (2003) Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences of the United States of America 100: 9128–9133.Google Scholar
Tar'an, B, Zhang, C, Warkentin, T, Tullu, A and Vandenberg, A (2005) Genetic diversity among varieties an wild species accessions of pea (Pisum sativum L.) based on molecular markers, and morphological and physiological characters. Genome 48: 257–272.CrossRefGoogle ScholarPubMed
Zimnoch-Guzowska E, Lojkowska E and Pérombelon M (2006) Resistance to bacterial pathogens. In: Razdan MK and Mattoo AK (eds) Genetic Improvement of Solanaceous Crops, Vol. 1: “Potato”. Enfield: Science Publishers, Inc., pp. 339–395.Google Scholar
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