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3 - Estimation of recombination frequencies

Published online by Cambridge University Press:  05 August 2013

J. W. Van Ooijen
Affiliation:
Kyazma B.V., Wageningen, The Netherlands
J. Jansen
Affiliation:
Biometris, Wageningen University and Research Centre, The Netherlands
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Summary

The rate of recombination between two loci is a measure of the distance these loci are apart on the chromosomes. Measuring these values for all loci is the starting point for estimating the map of the loci. In Chapter 2, the recombination frequency of two loci was introduced as the measure of genetic recombination: this is the proportion of gametes that are recombinant between the two loci in a single meiosis. A complication with higher plants and animals is that we do not observe the gametes but the diploid individuals in which two gametes are combined. Because in diploids two alleles are present for each locus, there is not always a one-to-one relationship between the observation of a locus and its genotype. Strictly speaking, we should use the term phenotype for observations regarding the genotype. In some cases, it is possible to determine exactly the genotype from the phenotype, for instance in the backcross. In such cases, estimates of recombination frequencies can be obtained by simple counting. We show that the same estimates are obtained by employing the maximum likelihood principle. This same principle can then also be employed in situations where there is no one-to-one relationship between phenotype and genotype. In yet other situations, recombinant genotypes are the result of several subsequent meioses. Here, the observed recombination must be translated into the probability of recombination in a single meiosis.

What do we observe? From separate loci to pairs, from phenotype to genotype

Recombination is a phenomenon that occurs with respect to pairs of genes or markers. In a regular linkage analysis, however, we start with observing the phenotypes of loci separately. Only as a next step are the observations combined into pairs, which allows the study of recombination. Let us first look at phenotypes: what exactly is a phenotype? The term phenotype was introduced by the Danish geneticist W. Johannsen back in 1909 in order to be able to make the distinction between what is observed of an organism and its genetic constitution. By definition, a phenotype is what we can observe of the genotype of an individual. Although somewhat confusing, the pleasant characteristic of many genetic markers is that the phenotype is equal to the genotype. This is why the words are regularly used as synonymous in the context of linkage analysis. However, there can be a major difference.

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Publisher: Cambridge University Press
Print publication year: 2013

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References

Bateson, W. (1909). Mendel's Principles of Heredity. Cambridge: Cambridge University Press. .CrossRefGoogle Scholar
Bulmer, M. G. (1985). The Mathematical Theory of Quantitative Genetics. Oxford: Clarendon Press.Google Scholar
Darvasi, A. & Soller, M. (1995). Advanced intercross lines, an experimental population for fine genetic mapping. Genetics, 141, 1199–207.Google ScholarPubMed
Dempster, A. P., Laird, N. M. & Rubin, D. B. (1977). Maximum likelihood from incomplete data via the EM algorithm. Journal of the Royal Statistical Society Series B, 39, 1–38.Google Scholar
Johannsen, W. (1909). Elemente der exakten Erblichkeitslehre. Jena: Gustav Fischer Verlag. .Google Scholar
Mather, K. (1936). Types of linkage data and their value. Annals of Eugenics, 7, 251–64.CrossRefGoogle Scholar
Mather, K. (1951). The Measurement of Linkage in Heredity. London: Methuen.Google Scholar
Punnett, R. C. (1905). Mendelism. Cambridge: Macmillan & Bowes.Google Scholar
Sturtevant, A. H. (1913). The linear arrangement of six sex-linked factors in Drosophila, as shown by their mode of association. Journal of Experimental Zoology, 14, 43–59. .CrossRefGoogle Scholar
Winkler, C. R., Jensen, N. M., Cooper, M., Podlich, D. W. & Smith, O. S. (2003). On the determination of recombination rates in intermated recombinant inbred populations. Genetics, 164, 741–5.Google ScholarPubMed

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