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Chapter 2 - Immunohistochemistry Quality Assurance and Quality Control

Published online by Cambridge University Press:  16 June 2022

By
Bronwyn Christiansen  and
Hazel Chambers
Edited by
Trung Nguyen
Trung Nguyen
Affiliation:
Peter MacCallum Cancer Centre, Australia
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Summary

Many parameters are associated with IHC testing assays. With so many variables, it is quite easy to accumulate errors within the system. To make things more manageable, these considerations are categorized into three main groups. Pre-analytic aspects occur before the assay, analytic factors are concerned with the staining protocol and post-analytic elements relate to interpreting of results. It has also come to reason that any one variable can impact the reliability and consistency of the overall IHC assay. In this regard, standardization requirements have been enlisted to assist laboratories achieve optimal results. In addition, monitoring proficiency testing regimens and various organizations are in place to ensure high levels of standards are attained. All these endeavours are known as quality assurance and quality control measures. They are arranged under the overall umbrella of a facility’s quality management system.

Keywords

pre-analyticanalyticpost-analyticvariablesparametersin vitro diagnosticresearch use onlystandardizationquality managementTherapeutic Goods Administration (TGA)
Type
Chapter
Information
Immunohistochemistry
A Technical Guide to Current Practices
 , pp. 24 - 66
Publisher: Cambridge University Press
Print publication year: 2022

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