This fully updated new edition of a successful and popular practical guide is an indispensable account of modern in-vitro fertilization practice. Initial chapters cover theoretical aspects of gametogenesis and embryo development at the cellular and molecular level, while the latter half of the book describes the requisites for a successful IVF laboratory and the basic technologies in ART. Advanced techniques, including pre-implantation genetic diagnosis, vitrification and stem-cell technology, are comprehensively covered, providing up-to-date analyses of these groundbreaking technologies. This edition includes:New practical techniques, including preservation of fertility for cancer patients, stem-cell biology/technology, vitrification and in-vitro maturationA 'refresher' study review of fundamental principles of cell and molecular biologyThe latest information available from animal and human research in reproductive biologyPacked with a wealth of practical and scientific detail, this is a must for all IVF practitioners.

Review of the previous edition:‘… an excellent handbook for good IVF laboratory practice right down to a description of total quality management (TQM) systems … concise, well written and should be mandatory reading for all potential and actual embryologists, as well as for those clinicians seeking a wider understanding of the subject.'

Source: Human Fertility

Review of the previous edition:‘The authors are masters of this work. Their views are up-to-date, practical, and well documented. This work will be of greatest interest to clinical practitioners of in vitro fertilization that want a relatively short and very precise overview of laboratory methods. It is highly recommended.'

John E. Buster Source: Fertility and Sterility