Book contents
- Frontmatter
- Contents
- List of contributors
- Foreword by Sidney Altman
- Foreword by Victor R. Ambros
- Introduction
- I Discovery of microRNAs in various organisms
- II MicroRNA functions and RNAi-mediated pathways
- III Computational biology of microRNAs
- IV Detection and quantitation of microRNAs
- 17 Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes
- 18 Detection and quantitation of microRNAs using the RNA Invader® assay
- 19 A single molecule method to quantify miRNA gene expression
- 20 Real-time quantification of microRNAs by TaqMan® assays
- 21 Real-time quantification of miRNAs and mRNAs employing universal reverse transcription
- V MicroRNAs in disease biology
- VI MicroRNAs in stem cell development
- Index
- Plate section
- References
18 - Detection and quantitation of microRNAs using the RNA Invader® assay
from IV - Detection and quantitation of microRNAs
Published online by Cambridge University Press: 22 August 2009
Edited by
Book contents
- Frontmatter
- Contents
- List of contributors
- Foreword by Sidney Altman
- Foreword by Victor R. Ambros
- Introduction
- I Discovery of microRNAs in various organisms
- II MicroRNA functions and RNAi-mediated pathways
- III Computational biology of microRNAs
- IV Detection and quantitation of microRNAs
- 17 Detection and analysis of microRNAs using LNA (locked nucleic acid)-modified probes
- 18 Detection and quantitation of microRNAs using the RNA Invader® assay
- 19 A single molecule method to quantify miRNA gene expression
- 20 Real-time quantification of microRNAs by TaqMan® assays
- 21 Real-time quantification of miRNAs and mRNAs employing universal reverse transcription
- V MicroRNAs in disease biology
- VI MicroRNAs in stem cell development
- Index
- Plate section
- References
Summary
Introduction
MicroRNAs are short single-stranded RNA molecules ranging in length from 17 to 24 nucleotides. During the past few years, hundreds of miRNAs have been identified in plants, animals, and a number of viruses with their exact biological function not fully understood (Lagos-Quintana et al., 2001; Lagos-Quintana et al., 2002; Reinhart et al., 2002; Hunter and Poethig, 2003; Lagos-Quintana et al., 2003; Pfeffer et al., 2004; Dunn et al., 2005; Pasquinelli et al., 2005; Wienholds and Plasterk, 2005; Wienholds et al., 2005). It has been shown that miRNAs target messenger RNA (mRNAs) at specific sites inducing cleavage of the RNA or result in inhibition of translation (Bartel, 2004). MiRNAs also exhibit unique expression patterns in tumor cells and therefore maybe useful as molecular markers for cancer cells (Michael et al., 2003; Calin et al., 2004; Croce and Calin, 2005; Eis et al., 2005). Moreover, microRNAs have been identified to be involved in regulation of cell and tissue development as well as several biological processes including cell proliferation and death, apoptosis, neuron development, DNA methylation and chromatin modification, and fat metabolism (Reinhart et al., 2000; Pasquinelli and Ruvkun, 2002; Ambros, 2003; Brennecke et al., 2003; Johnston and Hobert, 2003; Xu et al., 2003; Bao et al., 2004; Alvarez-Garcia and Miska, 2005; Croce and Calin, 2005; Miska, 2005).
Several methods of detecting and quantitating miRNAs have been developed. The size and sequence homology of some miRNAs makes their quantitation and differentiation challenging to conventional RT-PCR methods or standard microchip hybridization techniques.
- Type
- Chapter
- Information
- MicroRNAsFrom Basic Science to Disease Biology, pp. 242 - 254Publisher: Cambridge University PressPrint publication year: 2007
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