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8 - Separating autotrophic and heterotrophic components of soil respiration: lessons learned from trenching and related root-exclusion experiments

Published online by Cambridge University Press:  11 May 2010

By
Daniel Epron
Edited by
Werner L. Kutsch ,
Michael Bahn  and
Andreas Heinemeyer
Werner L. Kutsch
Affiliation:
Max-Planck-Institut für Biogeochemie, Jena
Michael Bahn
Affiliation:
Leopold-Franzens-Universität Innsbruck, Austria
Andreas Heinemeyer
Affiliation:
Stockholm Environmental Institute, University of York
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Summary

INTRODUCTION

Soil respiration (RS, Fig. 8.1) is the sum of an autotrophic component (RA) produced by roots and the associated rhizosphere (mycorrhizae and rhizosphere bacteria) and a heterotrophic component (RH) originating from soil micro-organisms that decompose the organic materials from both above-ground and below-ground litter (Bowden et al., 1993; Boone et al., 1998; Epron et al., 1999). Autotrophic respiration involves root carbohydrates and root exudates that have very low residence time in soil, while the heterotrophic component involves carbon compounds with a longer residence time, ranging from months to years for fresh litter and from years to centuries for old soil organic matter. All components are thought to be differently influenced by climatic conditions and site characteristics (Boone et al., 1998; Epron et al., 2001; Lavigne et al., 2004; Dilustro et al., 2005) and are thought to respond differently to elevated atmospheric CO2 or soil warming (Rustad et al., 2001; King et al., 2004; Pendall et al., 2004; Soe et al., 2004; Eliasson et al., 2005). Thus, separate estimations of these components are required for analyzing and modelling soil respiration and its response to climate or perturbation, for providing a better knowledge of carbon budgets of ecosystems and for improving carbon sequestration in soil (Hanson et al., 2000; Ryan and Law, 2005; Subke et al., 2006).

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Type
Chapter
Information
Soil Carbon Dynamics
An Integrated Methodology
 , pp. 157 - 168
Publisher: Cambridge University Press
Print publication year: 2010

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References

Andersen, C., Nikolov, I., Nikolova, P., Matyssek, R. and Häberle, K.-H. (2005) Estimating autotrophic belowground respiration in spruce and beech forests: decreases following girdling. European Journal of Forest Research, 124, 155–63.CrossRefGoogle Scholar
Andrews, J. A., Harrison, K. G., Matamala, R. and Schlesinger, W. H. (1999) Separation of root respiration from total soil respiration using carbon-13 labelling during free-air carbon dioxide enrichment (FACE). Soil Science Society of America Journal, 63, 1429–35.CrossRefGoogle Scholar
Bahn, M., Knapp, M., Garajova, Z., Pfahringer, N. and Cernusca, A. (2006) Root respiration in temperate mountain grasslands differing in land use. Global Change Biology, 12, 995–1006.CrossRefGoogle Scholar
Bhupinderpal-Singh, N. A., Löfvenius, M. O., Högberg, M. N., Mellander, and , P.-E., Högberg, P. (2003) Tree root and soil heterotrophic respiration as revealed by girdling of boreal Scots pine forest: extending observations beyond the first year. Plant, Cell and Environment, 26, 1287–96.CrossRefGoogle Scholar
Binkley, D., Stape, J. L., Takahashi, E. N. and Ryan, M. G. (2006) Tree-girdling to separate root and heterotrophic respiration in two Eucalyptus stands in Brazil. Oecologia, 148, 447–54.CrossRefGoogle Scholar
Bond-Lamberty, B., Wang, C. and Gower, S. T. (2004a) Contribution of root respiration to soil surface CO2 flux in a boreal black spruce chronosequence. Tree Physiology, 24, 1387–95.CrossRefGoogle Scholar
Bond-Lamberty, B., Wang, C. and Gower, S. T. (2004b) A global relationship between the heterotrophic and autotrophic components of soil respiration?Global Change Biology, 10, 1756–66.CrossRefGoogle Scholar
Boone, R. D., Nadelhoffer, K. J., Canary, J. D. and Kaye, J. P. (1998) Roots exert a strong influence on the temperature sensitivity of soil respiration. Nature, 396, 570–2.CrossRefGoogle Scholar
Bouma, T., Nielsen, K. L., Eissenstat, D. M. and Lynch, J. P. (1997) Estimating respiration of roots in soil: interactions with soil CO2, soil temperature and soil water content. Plant and Soil, 195, 221–32.CrossRefGoogle Scholar
Bowden, R. D., Nadelhoffer, K. J., Boone, R. D., Melillo, J. M. and Garrison, J. B. (1993) Contributions of aboveground litter, belowground litter, and root respiration to total soil respiration in a temperate mixed hardwood forest. Canadian Journal of Forest Research, 23, 1402–7.CrossRefGoogle Scholar
Brumme, R. (1995) Mechanisms of carbon and nutrient release and retention in beech forest gaps. III. Environmental regulation of soil respiration and nitrous oxide emissions along a microclimatic gradient. Plant and Soil, 168–9, 593–600.CrossRefGoogle Scholar
Burton, A. J., Pregitzer, K. S., Zogg, G. P. and Zak, D. R. (1998) Drought reduces root respiration in sugar maple forests. Ecological Applications, 8, 771–8.CrossRefGoogle Scholar
Cheng, W. (1996) Measurement of rhizosphere respiration and organic matter decomposition using natural 13C. Plant and Soil, 183, 263–8.CrossRefGoogle Scholar
Corbeels, M., McMurtrie, R. E., Pepper, D. A. and O'Connell, A. M. (2005) A process-based model of nitrogen cycling in forest plantations. Part I. Structure, calibration and analysis of the decomposition model. Ecological Modelling, 187, 426–48.CrossRefGoogle Scholar
Craine, J. M., Wedin, D. A. and Chapin III, F. S. (1999) Predominance of ecophysiological controls on soil CO2 flux in a Minnesota grassland. Plant and Soil, 207, 77–86.CrossRefGoogle Scholar
Davidson, E. A., Janssens, I. A. and Luo, Y. (2006) On the variability of respiration in terrestrial ecosystems: moving beyond Q10. Global Change Biology, 12, 154–64.CrossRefGoogle Scholar
Dewar, R. C. (1991) Analytical model of carbon storage in the trees, soils and wood products of managed forests. Tree Physiology, 8, 239–58.CrossRefGoogle ScholarPubMed
Dilustro, J. J., Collins, B., Duncan, L. and Crawford, C. (2005) Moisture and soil texture effects on soil CO2 efflux components in southeastern mixed pine forests. Forest Ecology and Management, 204, 85–95.CrossRefGoogle Scholar
Dörr, H. and Münnich, K. O. (1986) Annual variations of the 14C content of soil CO2. Radiocarbon, 28, 338–45.CrossRefGoogle Scholar
Ekblad, A. and Högberg, P. (2001) Natural abundance of 13C in CO2 respired from forest soils reveals speed of link between tree photosynthesis and root respiration. Oecologia, 127, 305–8.CrossRefGoogle ScholarPubMed
Eliasson, P. E., McMurtrie, R. E., Pepper, D. A.et al. (2005) The response of heterotrophic CO2 flux to soil warming. Global Change Biology, 11, 167–81.CrossRefGoogle Scholar
Epron, D., Farque, L., Lucot, E. and Badot, P. M. (1999) Soil CO2 efflux in a beech forest: the contribution of root respiration. Annals of Forest Science, 56, 289–95.CrossRefGoogle Scholar
Epron, D., Dantec, V., Dufrêne, E. and Granier, A. (2001) Seasonal dynamics of soil carbon dioxide efflux and simulated rhizosphere respiration in a beech forest. Tree Physiology, 21, 145–52.CrossRefGoogle Scholar
Epron, D., Nouvellon, Y., Deleporte, P.et al. (2006) Soil carbon balance in a clonal Eucalyptus plantation in Congo: effects of logging on carbon inputs and soil CO2 efflux. Global Change Biology, 12, 1021–31.CrossRefGoogle Scholar
Ewel, K. C., Cropper, W. P. and Gholz, H. L. (1987) Soil CO2 evolution in Florida slash pine plantations. II. Importance of root respiration. Canadian Journal of Forest Research, 17, 330–3.CrossRefGoogle Scholar
Fessenden, J. E. and Ehleringer, J. R. (2003) Temporal variation in δ13C of ecosystem respiration in the Pacific Northwest: links to moisture stress. Oecologia, 136, 129–36.CrossRefGoogle Scholar
Frey, B., Hagedorn, F. and Giudici, F. (2006) Effect of girdling on soil respiration and root composition in a sweet chestnut forest. Forest Ecology and Management, 225, 271–7.CrossRefGoogle Scholar
Gaudinski, J. B., Trumbore, S. E., Davidson, E. A. and Zheng, S. (2000) Soil carbon cycling in a temperate forest: radiocarbon-based estimates of residence times, sequestration rates and partitioning of fluxes. Biogeochemistry, 51, 33–69.CrossRefGoogle Scholar
Hanson, P. J., Edwards, N. T., Garten, C. T. and Andrews, J. A. (2000) Separating root and soil microbial contributions to soil respiration: a review of methods and observations. Biogeochemistry, 48, 115–46.CrossRefGoogle Scholar
Heinemeyer, A., Ineson, P., Ostle, N. and Fitter, A. H. (2006) Respiration of the external mycelium in the arbuscular mycorrhizal symbiosis shows strong dependence on recent photosynthates and acclimation to temperature. New Phytologist, 171, 159–70.CrossRefGoogle Scholar
Högberg, M. N. and Högberg, P. (2002) Extramatrical ectomycorrhizal mycelium contributes one-third of microbial biomass and produces, together with associated roots, half the dissolved organic carbon in a forest soil. New Phytologist, 154, 791–5.CrossRefGoogle Scholar
Högberg, M., Nordgren, A., Högberg, M. N.et al. (2005) Fractional contributions by autotrophic and heterotrophic respiration to soil-surface CO2 efflux in boreal forests. In The Carbon Balance of Forest Biomes, ed. Griffiths, H. and Jarvis, P. G.. Taylor & Francis Group, pp. 251–67.Google Scholar
Högberg, P., Nordgren, A., Buchmann, N.et al. (2001) Large-scale forest girdling shows that current photosynthesis drives soil respiration. Nature, 411, 789–92.CrossRefGoogle ScholarPubMed
Janssens, I. A., Lankreijer, H., Metteucci, G.et al. (2001) Productivity overshadows temperature in determining soil and ecosystem respiration across European forests. Global Change Biology, 7, 269–78.CrossRefGoogle Scholar
Jenkinson, D. S. (1990) The turnover of organic carbon and nitrogen in soil. Philosophical Transactions of the Royal Society London B, 329, 361–8.CrossRefGoogle Scholar
Johnson, D., Leake, J. R. and Read, D. J. (2001) Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks. New Phytologist, 152, 555–62.CrossRefGoogle Scholar
King, J. S., Hanson, P. J., Bernhardt, E.et al. (2004) A multiyear synthesis of soil respiration responses to elevated atmospheric CO2 from four forest FACE experiments. Global Change Biology, 10, 1027–42.CrossRefGoogle Scholar
Kucera, C. L. and Kirham, R. (1971) Soil respiration studies in tallgrass prairie in Missouri. Ecology, 52, 912–15.CrossRefGoogle Scholar
Kutsch, W. L., Staack, A., Wötzel, J., Middlehoff, U. and Kappen, L. (2001) Field measurements of root respiration and total soil respiration in an alder forest. New Phytologist, 150, 157–68.CrossRefGoogle Scholar
Kuzyakov, Y. (2006) Sources of CO2 efflux from soil and review of partitioning methods. Soil Biology and Biochemistry, 38, 425–48.CrossRefGoogle Scholar
Lamade, E., Djegui, N. and Leterme, P. (1996) Estimation of carbon allocation to the roots from soil respiration measurements of oil palm. Plant and Soil, 181, 329–39.CrossRefGoogle Scholar
Lavigne, M. B., Boutin, R., Foster, R. J.et al. (2003) Soil respiration responses to temperature are controlled more by roots than by decomposition in balsam fir ecosystems. Canadian Journal of Forest Research, 33, 1744–53.CrossRefGoogle Scholar
Lavigne, M. B., Foster, R. J. and Goodine, G. (2004) Seasonal and annual changes in soil respiration in relation to soil temperature, water potential and trenching. Tree Physiology, 24, 415–24.CrossRefGoogle ScholarPubMed
Law, B. E., Thornton, P. E., Irvine, J., Anthoni, P. M. and Tuyl, S. (2001) Carbon storage and fluxes in ponderosa pine forests at different developmental stages. Global Change Biology, 7, 755–77.CrossRefGoogle Scholar
Lee, M.-S., Nakane, K., Nakatsubo, T. and Koizumi, H. (2003) Seasonal changes in the contribution of root respiration to total soil respiration in a cool-temperate deciduous forest. Plant and Soil, 255, 311–18.CrossRefGoogle Scholar
Lin, G., Ehleringer, J. R., Rygiewicz, P. T., Johnson, M. G. and Tingey, D. T. (1999) Elevated CO2 and temperature impacts on different components of soil CO2 efflux in Douglas-fir terracosms. Global Change Biology, 5, 157–68.CrossRefGoogle Scholar
Maier, C. A. and Kress, L. W. (2000) Soil CO2 evolution and root respiration in 11 year-old loblolly pine (Pinus taeda) plantations as affected by moisture and nutrient availability. Canadian Journal of Forest Research, 30, 347–59.CrossRefGoogle Scholar
Nakane, K., Yamamoto, M. and Tsubota, H. (1983) Estimation of root respiration rate in a mature forest ecosystem. Japanese Journal of Ecology, 33, 397–408.Google Scholar
Nakane, K., Kohno, T. and Horikoshi, T. (1996) Root respiration rate before and just after clear-felling in a mature, deciduous, broad-leaved forest. Ecological Research, 11, 111–19.CrossRefGoogle Scholar
Ngao, J., Epron, D., Brechet, C. and Granier, A. (2005) Estimating the contribution of leaf litter decomposition to soil CO2 efflux in a beech forest using 13C-depleted litter. Global Change Biology, 11, 1768–76.CrossRefGoogle Scholar
O'Connell, K. E. B., Gower, S. T. and Norman, J. M. (2003) Net ecosystem production of two contrasting boreal black spruce forest communities. Ecosystems, 6, 248–60.CrossRefGoogle Scholar
Ohashi, M., Gyokusen, K. and Saito, A. (2000) Contribution of root respiration to total soil respiration in a Japanese cedar (Cryptomeria japonica D. Don) artificial forest. Ecological Research, 15, 323–33.CrossRefGoogle Scholar
Olsson, P., Linder, S., Giesler, R. and Högberg, P. (2005) Fertilization of boreal forest reduces both autotrophic and heterotrophic soil respiration. Global Change Biology, 11, 1745–53.CrossRefGoogle Scholar
Parton, W. J., Schimel, D. S., Cole, C. V. and Ojima, D. S. (1987) Analysis of factors controlling soil organic matter levels in Great Plains grasslands. Soil Science of American Journal, 51, 1173–9.CrossRefGoogle Scholar
Pendall, E., Bridgham, S., Hanson, P. J.et al. (2004) Below-ground process responses to elevated CO2 and temperature: a discussion of observations, measurement methods, and models. New Phytologist, 162, 311–22.CrossRefGoogle Scholar
Rochette, P. and Flanagan, L. B. (1997) Quantifying rhizosphere respiration in a corn crop under field conditions. Soil Science Society of America Journal, 61, 466–74.CrossRefGoogle Scholar
Rochette, P., Flanagan, L. B. and Gregorich, E. G. (1999) Separating soil respiration into plant and soil components using analyses of the natural abundance of carbon-13. Soil Science Society of America Journal, 63, 1207–13.CrossRefGoogle Scholar
Rustad, L. E., Campbell, J. L., Marion, G. M.et al. (2001) A meta-analysis of the response of soil respiration, net nitrogen mineralization, and aboveground plant growth to experimental ecosystem warming. Oecologia, 126, 543–62.CrossRefGoogle ScholarPubMed
Ryan, M. G. and Law, B. E. (2005) Interpreting, measuring, and modelling soil respiration. Biogeochemistry, 73, 3–27.CrossRefGoogle Scholar
Ryan, M. G., Hubbard, R. M., Pongracic, S., Raison, R. J. and McMurtrie, R. E. (1996) Foliage, fine-root, woody-tissue and stand respiration in Pinus radiata in relation to nitrogen status. Tree Physiology, 16, 333–43.CrossRefGoogle ScholarPubMed
Saiz, G., Byrne, K., Butterbach-Bahl, K.et al. (2006) Stand age-related effects on soil respiration in a first rotation Sitka spruce chronosequence in central Ireland. Global Change Biology, 12, 1007–20.CrossRefGoogle Scholar
Schuur, E. A. G. and Trumbore, S. E. (2006) Partitioning sources of soil respiration in boreal black spruce forest using radiocarbon. Global Change Biology, 12, 165–76.CrossRefGoogle Scholar
Scott-Denton, L. E., Rosenstiel, T. N. and Monson, R. K. (2006) Differential controls by climate and substrate over the heterotrophic and rhizospheric components of soil respiration. Global Change Biology, 12, 205–16.CrossRefGoogle Scholar
Soe, A. R. B., Giesemann, A., Anderson, T.-H., Weigel, H.-J. and Buchmann, N. (2004) Soil respiration under elevated CO2 and its partitioning into recently assimilated and older carbon sources. Plant and Soil, 262, 85–94.CrossRefGoogle Scholar
Striegl, R. G. and Wickland, K. P. (1998) Effects of a clear-cut harvest on soil respiration in a jack pine–lichen woodland. Canadian Journal of Forest Research, 28, 534–9.CrossRefGoogle Scholar
Subke, J.-A., Hahn, V., Battipaglia, G.et al. (2004) Feedback interactions between needle litter decomposition and rhizosphere activity. Oecologia, 139, 551–9.CrossRefGoogle ScholarPubMed
Subke, J.-A., Inglima, I. and Cotrufo, M. F. (2006) Trends and methodological impacts in soil CO2 efflux partitioning: a metaanalytical review. Global Change Biology, 12, 921–43.CrossRefGoogle Scholar
Swinnen, J. (1994) Evaluation of the use of a model rhizodeposition technique to separate root and microbial respiration in soil. Plant and Soil, 165, 89–101.CrossRefGoogle Scholar
Tate, K. R., Ross, D. J., O'Brien, B. J. and Kelliher, F. M. (1993) Carbon storage and turnover, and respiratory activity, in the litter and soil of an old-growth southern beech (Nothofagus) forest. Soil Biology and Biochemistry, 25, 1601–12.CrossRefGoogle Scholar
Thierron, V. and Laudelout, H. (1996) Contribution of root respiration to total CO2 efflux from the soil of a deciduous forest. Canadian Journal of Forest Research, 26, 1142–8.CrossRefGoogle Scholar
Trumbore, S. (2000) Age of soil organic matter and soil respiration: radiocarbon constraints on belowground dynamics. Ecological Applications, 10, 399–411.CrossRefGoogle Scholar
Verburg, P. S. J., Arnone, J. A., Obrist, D.et al. (2004) Net ecosystem carbon exchange in two experimental grassland ecosystems. Global Change Biology, 10, 498–508.CrossRefGoogle Scholar
Wan, S. and Luo, Y. (2003) Substrate regulation of soil respiration in a tallgrass prairie: results of a clipping and shading experiment. Global Biogeochemical Cycles, 17, 1–12.CrossRefGoogle Scholar

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