We derive expressions for the generating function of the equilibrium queue length probability distribution in a single server queue with general service times and independent Poisson arrival streams of both ordinary, positive customers and negative customers which eliminate a positive customer if present. For the case of first come first served queueing discipline for the positive customers, we compare the killing strategies in which either the last customer in the queue or the one in service is removed by a negative customer. We then consider preemptive-restart with resampling last come first served queueing discipline for the positive customers, combined with the elimination of the customer in service by a negative customer—the case of elimination of the last customer yields an analysis similar to first come first served discipline for positive customers. The results show different generating functions in contrast to the case where service times are exponentially distributed. This is also reflected in the stability conditions. Incidently, this leads to a full study of the preemptive-restart with resampling last come first served case without negative customers. Finally, approaches to solving the Fredholm integral equation of the first kind which arises, for instance, in the first case are considered as well as an alternative iterative solution method.