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Effect of rearing environment and dietary zinc oxide on the response of group-housed weaned pigs to enterotoxigenic Escherichia coli O149 challenge

  • R. D. Slade (a1), I. Kyriazakis (a2), S. M. Carroll (a1), F. H. Reynolds (a1), I. J. Wellock (a3), L. J. Broom (a1) and H. M. Miller (a1)...

Abstract

A 2 × 2 factorial experiment was conducted to determine the effects of rearing environment (indoor (In) v. outdoor (Out)) and dietary zinc oxide (ZnO) supplementation (0 (−Zn) v. 3100 (+Zn) mg/kg feed) on the response of weaned pigs to a challenge infection with enterotoxigenic Escherichia coli (ETEC). Pigs from the two rearing environments were weaned onto trial diets at 4 weeks of age, moved into conventional accommodation and infected 3 days later with 109 CFU ETEC per os. Faecal ETEC shedding was determined before and after challenge. After 7 days of ETEC infection, all pigs were euthanized for gut lactic acid bacteria (LAB)-to-coliform ratio, pH and small intestine morphological measurements. Both ZnO and outdoor rearing reduced ETEC excretion, and these effects were additive. Outdoor rearing increased small intestine and colon tissue weight. ZnO increased villus height and goblet cell number in the upper small intestine, LAB-to-coliform ratio (through reduced coliforms) in the lower small intestine and proximal colon, and improved growth performance. There were interactive effects of rearing environment and ZnO supplementation on upper small intestine villus height and daily gain, as outdoor rearing conferred advantages on these variables only with ZnO dietary supplementation. Daily gains were 233, 174, 277 and 347 (s.e.m. 27.2) g/day for the In − Zn, Out − Zn, In + Zn and Out + Zn, respectively. These results suggest different, but complementary mechanisms of intestinal health and performance in outdoor-reared pigs and those offered ZnO supplemented diets. The results indicate that the benefits of ZnO to the weaned pig extend beyond suppression of ETEC and appear mediated through altered development of the small intestine mucosa.

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References

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Blomberg, L, Krivan, HC, Cohen, PS, Conway, PL 1993. Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae. Infection and Immunity 61, 25262531.
Blomberg, L, Gustafsson, L, Cohen, PS, Conway, PL, Blomberg, A 1995. Growth of Escherichia coli K88 in piglet ileal mucosa: protein expression as an indicator of type of metabolism. Journal of Bacteriology 177, 66956703.
Carlson, D, Poulsen, HD, Vestergaard, M 2004. Additional dietary zinc for weaning piglets is associated with elevated concentrations of serum IGF-1. Journal of Animal Physiology and Animal Nutrition 88, 332339.
Case, CL, Carlson, MS 2002. Effect of feeding organic and inorganic sources of additional zinc on growth performance and zinc balance in nursery pigs. Journal of Animal Science 80, 19171924.
Crane, JK, Naeher, TM, Shulgina, I, Zhu, C, Boedeker, EC 2007. Effect of zinc in enteropathogenic Escherichia coli infection. Infection and Immunity 75, 59745984.
Dean-Nystrom, EA, Samuel, JE 1994. Age related resistance to 987P fimbria mediated colonization correlates with specific glycolipid receptors in intestinal mucus in swine. Infection and Immunity 62, 47894794.
Gentry, JG, McGlone, JJ, Miller, MF, Blanton, JR 2002. Diverse birth and rearing environment effects on pig growth and meat quality. Journal of Animal Science 80, 17071715.
Hahn, JD, Baker, DH 1993. Growth and plasma zinc responses of young pigs fed pharmacological levels of zinc. Journal of Animal Science 71, 30203024.
Hampson, DJ 1986. Attempts to modify changes in the piglet small intestine after weaning. Research in Veterinary Science 40, 313317.
Hedemann, MS, Jensen, BB, Poulsen, HD 2006. Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs. Journal of Animal Science 84, 33103320.
Heo, JM, Kim, JC, Hansen, CF, Mullan, BP, Hampson, DJ, Pluske, JR 2009. Feeding a diet with decreased protein content reduces indices of protein fermentation and the incidence of post weaning diarrhea in weaned pigs challenged with an enterotoxigenic strain of Escherichia coli. Journal of Animal Science 87, 28332843.
Hill, GM, Mahan, DC, Carter, SD, Cromwell, GL, Ewan, RC, Harrold, RL, Lewis, AJ, Miller, PS, Shurson, GC, Veum, TL 2001. Effect of pharmacological concentrations of zinc oxide with and without the inclusion of an antibacterial agent on nursery pig performance. Journal of Animal Science 79, 934941.
Hojberg, O, Canibe, N, Poulsen, HD, Hedemann, MS, Jensen, BB 2005. Influence of dietary zinc oxide and copper sulfate on the gastrointestinal ecosystem in newly weaned piglets. Applied and Environmental Microbiology 71, 22672277.
Ilsley, SE, Miller, HM, Kamel, C 2005. Effects of dietary quillaja saponin and curcumin on the performance and immune status of weaned piglets. Journal of Animal Science 83, 8288.
Jin, LZ, Marquardt, RR, Baidoo, SK 2000. Inhibition of enterotoxigenic Escherichia coli K88, K99 and 987P by the Lactobacillus isolates from porcine intestine. Journal of the Science of Food and Agriculture 80, 619624.
Katouli, M, Melin, L, Jensen-Waern, M, Wallgren, P, Mollby, R 1999. The effect of zinc oxide on the stability of the intestinal flora with special reference to composition of coliforms in weaned pigs. Journal of Applied Microbiology 87, 564573.
Kelly, D, King, TP 2001. Digestive physiology and development in pigs. In The weaner pig: nutrition and management (ed. MA Varley and J Wiseman), pp. 179206. CABI Publishing, UK.
Kelly, D, Begbie, R, King, TP 1992. Postnatal intestinal development. In Neonatal survival and growth (ed. MA Varley, PEV Williams and TLJ Lawrence), pp. 6379. British Society of Animal Production – Occasional Publication no. 15, UK.
Kyriazakis, I, Houdijk, JGM 2007. Food intake and performance of pigs during health, disease and recovery. In Paradigms in pig science (ed. J Wiseman, MA Varley, S McOrist and B Kemp), pp. 493513. Nottingham University Press, Nottingham, UK.
Li, BT, Van Kessel, AG, Caine, WR, Huang, SX, Kirkwood, RN 2001. Small intestinal morphology and bacterial populations in the ileal digesta and feces of newly weaned pigs receiving a high dietary level of zinc oxide. Canadian Journal of Animal Science 81, 511516.
Li, XL, Yin, JD, Li, DF, Chen, XJ, Zang, JJ, Zhou, X 2006. Dietary supplementation with zinc oxide increases IGF-1 and IGF-1 receptor gene expression in the small intestine of the weanling piglets. Journal of Nutrition 136, 17861791.
Madec, F, Bridoux, N, Bounaix, S, Cariolet, R, Duval-Iflah, Y, Hampson, DJ, Jestin, A 2000. Experimental models of porcine post-weaning colibacillosis and their relationship to post-weaning diarrhoea and digestive disorders as encountered in the field. Veterinary Microbiology 72, 295310.
Mavromichalis, I, Peter, CM, Parr, TM, Ganessunker, D, Baker, DH 2000. Growth-promoting efficiency in young pigs of two sources of zinc oxide having either a high or low bioavailability of zinc. Journal of Animal Science 78, 28962902.
Miller, HM, Toplis, P, Slade, RD 2009. Can outdoor rearing and increased weaning age compensate for the removal of in-feed antibiotic growth promoters and zinc oxide? Livestock Science 125, 121131.
Miller, BG, Newby, TJ, Stokes, CR, Bourne, FJ 1984. Influence of diet on post-weaning malabsorption and diarrhoea in the pig. Research in Veterinary Science 36, 187193.
Miller, HM, Carroll, SM, Reynolds, FH, Slade, RD 2007. Effect of rearing environment and age on gut development of piglets at weaning. Livestock Science 108, 124127.
Mulder, IE, Schmidt, B, Stokes, CR, Lewis, M, Bailey, M, Aminov, RI, Prosser, JI, Gill, BP, Pluske, JR, Mayer, CD, Musk, CC, Kelly, D 2009. Environmentally-acquired bacteria influence microbial diversity and natural innate immune responses at gut surfaces. BMC Biology 7, 79.
Owusu-Asiedu, A, Nyachoti, CM, Marquardt, RR 2003. Response of early weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody, zinc oxide, fumaric acid, or antibiotic. Journal of Animal Science 81, 17901798.
Ragland, D, Schneider, JR, Amas, SF, Hill, M 2006. Alternatives to the use of antimicrobial feed additives in nursery diets: a pilot study. Journal of Swine Health and Production 14, 8288.
Roselli, M, Finamore, A, Garaguso, I, Britti, MS, Mengheri, E 2003. Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli. Journal of Nutrition 133, 40774082.
Tarnow, P, Argen, M, Steenfos, H, Jansson, JO 1994. Topical zinc oxide treatment increases endogenous gene expression of insulin-like growth factor-1 in granulation tissue from porcine wounds. Scandinavian Journal of Plastic and Reconstructive Surgery 28, 255259.
Van Dijk, AJ, Enthoven, PMM, Van den Hoven, SGC, Van Laarhoven, MMMH, Niewold, TA, Nabuurs, MJA, Benyen, AC 2002. The effect of dietary spray-dried porcine plasma on clinical response in weaned piglets challenged with a pathogenic Escherichia coli. Veterinary Microbiology 84, 207218.
Vente-Spreeuwenberg, MAM, Beynen, AC 2003. Diet modulation of small intestine integrity in weaned piglets. In Weaning the pig: concepts and consequences (ed. JR Pluske, J Le Dividich and MWA Verstegen), pp. 145198. Wageningen Academic Publishers, The Netherlands.
Wellock, IJ, Fortomaris, PD, Houdijk, JGM, Kyriazakis, I 2006. The effect of dietary protein supply on the performance and risk of post-weaning enteric disorders in newly weaned pigs. Animal Science 82, 327335.
Wellock, IJ, Fortomaris, PD, Houdijk, JGM, Kyriazakis, I 2008a. The interaction between dietary protein supply, weaning age and enterotoxigenic Escherichia coli infection on the performance and health of newly weaned pigs: performance. Animal 2, 834842.
Wellock, IJ, Fortomaris, PD, Houdijk, JGM, Kyriazakis, I 2008b. The interaction between dietary protein supply, weaning age and enterotoxigenic Escherichia coli infection on the performance and health of newly weaned pigs: health. Animal 2, 825833.
Wulbers-Mindermann, M, Algers, B, Berg, C, Lundeheim, N, Sigvardsson, J 2002. Primiparous and multiparous maternal ability in sows in relation to indoor and outdoor farrowing systems. Livestock Production Science 73, 285297.

Keywords

Effect of rearing environment and dietary zinc oxide on the response of group-housed weaned pigs to enterotoxigenic Escherichia coli O149 challenge

  • R. D. Slade (a1), I. Kyriazakis (a2), S. M. Carroll (a1), F. H. Reynolds (a1), I. J. Wellock (a3), L. J. Broom (a1) and H. M. Miller (a1)...

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