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The effects of seaweed extract inclusion on gut morphology, selected intestinal microbiota, nutrient digestibility, volatile fatty acid concentrations and the immune status of the weaned pig

  • P. Reilly (a1), J. V. O’Doherty (a1), K. M. Pierce (a1), J. J. Callan (a1), J. T. O’Sullivan (a2) and T. Sweeney (a1)...
Abstract

An experiment (complete randomised design) was conducted to investigate the effects of Laminaria hyperborea and Laminaria digitata seaweed extract inclusion on gut morphology, selected intestinal microbiota populations, volatile fatty acid concentrations and the immune status of the weaned pig. Twenty-eight piglets (24 days of age, 6.5 ± 1.4 kg live weight) were assigned to one of four dietary treatments for 7 days and then sacrificed: (T1) basal diet (control); (T2) basal diet and 1.5 g/kg L. hyperborea seaweed extract; (T3) basal diet and 1.5 g/kg L. digitata seaweed extract; and (T4) basal diet and 1.5 g/kg of a combination of L. hyperborea and L. digitata seaweed extract. The seaweed extract contained both laminarin and fucoidan. Digesta samples were taken from the caecum and colon to measure the enterobacteria, bifidobacteria and lactobacilli populations and for volatile fatty acid analysis. Tissue samples were taken from the duodenum, jejunum and ileum for morphological examination. Blood samples were taken to determine the cytokine gene expression profile and to measure the phagocytotic capacity of the blood. Pigs offered diets containing L. hyperborea seaweed extract had less bifidobacteria in the colon (P < 0.05) and lactobacilli in the caecum (P < 0.05) and colon (P < 0.001). The inclusion of L. digitata seaweed extract resulted in lower populations of enterobacteria in the caecum and colon (P < 0.01), bifidobacteria in the caecum (P < 0.05), and lactobacilli in the caecum (P < 0.05) and colon (P < 0.001). Pigs offered the combination of L. hyperborea and L. digitata seaweed extracts had less enterobacteria (P < 0.05) and lactobacilli (P < 0.01) in the caecum and colon. Pigs offered the L. digitata-supplemented diet had a reduced villous height in the duodenum and jejunum (P < 0.05). The inclusion of the L. digitata seaweed extract increased the molar proportion of butyric acid in the colon (P < 0.05). There was a significant reduction in the ammonia concentration in the colon with the inclusion of L. hyperborea (P < 0.01) and L. digitata (P < 0.05) seaweed extracts. An increase in the expression of the Interleukin-8 mRNA was observed on day 6 with the supplementation of the combination of L. hyperborea and L. digitata seaweed extract (P < 0.05). The inclusion of L. hyperborea seaweed extract resulted in an increase in total monocyte number (P < 0.05). In conclusion, the supplementation of L. hyperborea and L. digitata seaweed extract alone and in combination reduced the enterobacteria, bifidobacteria and lactobacilli populations in the caecum and colon, while only marginal effects on the immune response was observed.

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Corresponding author
E-mail: john.vodoherty@ucd.ie
References
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Annan, WD, Hirst, EL, Manners, DJ 1965. The constitution of laminarin. Part V. The location of 1,6-glucosidic linkages. Journal of the Chemical Society 885891.
Association of Official Analytical Chemists 1995. Official methods of analysis, 16th edition.AOAC, Washington, DC, USA.
Berteau, O, Mulloy, B 2003. Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology 13, 2940.
Brown, GD, Gordon, S 2005. Immune recognition of fungal β-glucans. Cellular Microbiology 7, 471479.
Conway, EJ 1957. Microdiffusion analysis and volumetric error. Crosby, Lockwood and Son, London.
De Lange, CFM 2000. Characterisation of the nonstarch polysaccharides. In Feed evaluation – principles and practice (ed. PJ Moughan, MWA Verstegen and MI Visser-Reyneveld), pp. 7792. Wageningen Pers, Wageningen, The Netherlands.
Deville, C, Damas, J, Forget, P, Dandrifosse, G, Peulen, O 2004. Laminarin in the dietary fibre concept. Journal of the Science of Food and Agriculture 84, 10301038.
Deville, C, Gharbi, M, Dandrifosse, G, Peulen, O 2007. Study on the effects of laminarin, a polysaccharide from seaweed, on gut characteristics. Journal of the Science of Food and Agriculture 87, 17171725.
Drew, MD, Van Kessel, AG, Estrada, AE, Ekpe, ED, Zijlstra, RT 2002. Effect of dietary cereal on intestinal bacterial populations in weaned pigs. Canadian Journal of Animal Science 82, 607609.
Estrada, A, Drew, MD, Van Kessel, A 2000. Effect of the dietary supplementation of fructooligosaccharides and Bifidobacterium longum to early-weaned pigs on performance and fecal bacterial populations. Canadian Journal of Animal Science 81, 141148.
Gardiner, GE, Campbell, AJ, O’Doherty, JV, Pierse, E, Lynch, PB, Leonard, FC, Stanton, C, Ross, RP, Lawlor, PG 2008. Effects of Ascophyllum nodosum extract on growth performance, digestibility, carcass characteristics and selected intestinal microflora populations of grower-finisher pigs. Animal Feed Science and Technology 141, 259273.
Goni, I, Gudiel-Urbano, M, Bravo, L, Saura-Calixto, F 2001. Dietary modulation of bacterial fermentative capacity by edible seaweeds in rats. Journal Agriculture Food Chemistry 49, 26632668.
Grinstead, GS, Tokach, MD, Dritz, SS, Goodband, RD, Nelssen, JL 2000. Effects of Spirulina platensis on growth performance of weanling pigs. Animal Feed Science and Technology 83, 237247.
Hampson, DJ 1986. Attempts to modify changes in the piglet small intestine after weaning. Research in Veterinary Science 40, 313317.
Jaskari, J, Kontula, P, Siitonen, A, Jousimies-Somer, H, Mattila-Sandholm, T, Poutanen, K 1998. Oat β-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains. Applied Microbial Biotechnology 49, 175181.
Kogan, G, Kocher, A 2007. Role of yeast cell wall polysaccharides in pig nutrition and health protection. Livestock Science 109, 161165.
Li, J, Li, DF, Xing, JJ, Cheng, ZB, Lai, CH 2006. Effects of β-glucan extracted from Saccharomyces cerevisiae on growth performance, and immunological and somatotropic responses of pigs challenged with Escherichia coli lipopolysaccharide. Journal of Animal Science 84, 23742381.
Marais, M-F, Joseleau, J-P 2001. A fucoidan fraction from Ascophyllum nodosum. Carbohydrate Research 336, 155159.
Mathers, JC, Annison, EF 1993. Stoichiometry of polysaccharide fermentation in the large intestine. In Dietary fibre and beyond Australian perspectives (ed. S Samman and G Annison), pp. 123135. Nutrition Society of Australia Occasional Publications, Perth, Australia.
McDonald, DE, Pethick, DW, Mullan, BP, Hampson, DJ 2001. Increasing viscosity of the intestinal contents alters small intestinal structure and intestinal growth, and stimulate proliferation of enterotoxigenic E. coli in newly-weaned pigs. British Journal of Nutrition 86, 487498.
Michel, C, Lahaye, M, Bonnet, C, Mabeau, S, Barry, J-L 1996. In vitro fermentation by human faecal bacteria of total and purified dietary fibres from brown seaweeds. British Journal of Nutrition 75, 263280.
Michel, C, Bernard, C, Lahaye, M, Formaglio, D, Kaeffer, B, Quemener, B, Berot, S, Yvin, JC, Blottiere, HM, Cherbut, C 1999. Algal oligosaccharides as functional foods: in vitro study of their cellular and fermentative effects. Food Science 19, 311332.
Mirelman, D, Altmann, G, Eshdat, Y 1980. Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. Journal of Clinical Microbiology 11, 328331.
Montagne, L, Pluske, JR, Hampson, DJ 2003. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal Feed Science and Technology 108, 95117.
Nabuurs, MJA, Hoogendoorn, A 1993. Villus height and crypt depth in weaned and unweaned pigs, reared under various circumstances in the Netherlands. Research in Veterinary Science 55, 7884.
NRC (National Research Council) 1998. Nutrient requirements of swine, 10th edition.National Academic Press, Washington, DC.
Pierce, KM, Callan, JJ, McCarthy, P, O’ Doherty, JV 2005. Performance of weanling pigs offered low or high lactose diets supplemented with avilamycin or inulin. Animal Science 80, 313318.
Pluske, JR, Hampson, DJ, Williams, IH 1997. Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livestock Production science 51, 215236.
Read, SM, Currie, G, Bacic, A 1996. Analysis of the structural heterogeneity of laminarian by electrospray-ionisation-mass spectrometry. Carbohydrate Research 281, 187201.
Roediger, WEW 1982. Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroentemology 83, 424429.
Statistical Analysis System (SAS) 1985. Statistical analysis systems. SAS Institute Inc., North Carolina, USA.
Shibata, H, Limuro, M, Uchiya, N, Kawamori, T, Nagaoka, M, Ueyama, S, Hashimoto, S, Yokokura, T, Sugimura, T, Wakabayashi, K 2003. Preventive effects of Cladosiphon fucoidan against Helicobacter pylori in Mongolian gerbils. Helicobacter 8, 5965.
Smith, JG, German, JB 1995. Molecular and genetic effects of dietary derived butyric acid. Food technology 49, 8790.
Usov, AI, Smirnova, GP, Klochkova, NG 2001. Polysaccharides of Algae: 55.1Polysaccharide composition of several brown algae from Kamchatka. Russian Journal of Bioorganic Chemistry 27, 444448.
Van Soest, PJ, Robertson, JB, Lewis, BA 1991. Methods of dietary fibre, neutral detergent fibre and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833597.
Williams, CH, David, DJ, Iismaa, O 1962. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Animal Science 59, 381385.
Williams, BA, Verstegen, MA, Tamminga, S 2001. Fermentation in the large intestine of singlestomached animals and its relationship to animal health. Nutrition Research Reviews 14, 207227.
Yun, C-H, Estrada, A, Van Kessel, A, Park, B-C, Laarveld, B 2003. β-Glucan, extracted from oat, enhances disease resistance against bacterial and parasitic infections. FEMS Immunology and Medical Microbiology 35, 6775.
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