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Expression of heat shock protein 70 in the liver of extensively and intensively kept heavy pigs

Published online by Cambridge University Press:  27 March 2013

E. Negrato ,
G. Di Martino ,
M. Vascellari ,
G. Radaelli ,
K. Capello ,
F. Pascoli ,
D. Bertotto  and
L. Bonfanti
E. Negrato
Affiliation:
Dipartimento di Biomedicina Comparata e Alimentazione, University of Padova, Viale dell'Università 16, 35020 Legnaro, Italy
G. Di Martino*
Affiliation:
Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Padova, Italy
M. Vascellari
Affiliation:
Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Padova, Italy
G. Radaelli
Affiliation:
Dipartimento di Biomedicina Comparata e Alimentazione, University of Padova, Viale dell'Università 16, 35020 Legnaro, Italy
K. Capello
Affiliation:
Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Padova, Italy
F. Pascoli
Affiliation:
Dipartimento di Biomedicina Comparata e Alimentazione, University of Padova, Viale dell'Università 16, 35020 Legnaro, Italy
D. Bertotto
Affiliation:
Dipartimento di Biomedicina Comparata e Alimentazione, University of Padova, Viale dell'Università 16, 35020 Legnaro, Italy
L. Bonfanti
Affiliation:
Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro, Padova, Italy
*
†E-mail: gdimartino@izsvenezie.it
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Abstract

The objective of this work was to investigate the expression of heat shock protein 70 (HSP70) by Western blot (WB) in swine liver. Subsequently, the study aimed to apply this method to two experimental groups of heavy pigs raised in different confinement systems: intensive/indoor (Group A) and extensive/outdoor (Group B). Thirty-six crossbred commercial heavy pigs were divided as follows: Group A (eight castrated males and eight females) was equally distributed into two single-sex indoor pens (1.02 m2/pig); Group B (11 castrated males and nine females) was kept in one single (partially grassy and partially wooded) open area of about 6000 m2. Group A was slaughtered at 41 weeks of age (170 ± 9 kg) and Group B at 48 weeks of age (172 ± 13 kg). At the abattoir the livers of all the animals were collected and analyzed by WB assay in order to quantify the levels of HSP70. Moreover, a further liver sample was taken from the same animals in order to investigate the cellular localization of HSP70 by immunohistochemistry (IHC). The interaction between sex and group resulted statistically significant (P = 0.001). When stratified by sex, Group A showed significantly higher HSP70 values compared with Group B for both male and female subjects (P < 0.001). Stratifying by group, males showed significantly higher HSP70 values than females in Group A (P < 0.001), whereas no statistical differences were observed between sexes for Group B (P = 0.653). The IHC results evidenced cytoplasmic immunoreactivity in a granular pattern in both groups. The different expression pattern observed by WB could prove to be a useful tool in the assessment of pig health and welfare.

Keywords

HSP70Western blotliverIHCheavy pig
Type
Behaviour, welfare and health
Information
animal , Volume 7 , Issue 8 , August 2013 , pp. 1362 - 1366
Copyright
Copyright © The Animal Consortium 2013 

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