Skip to main content
    • Aa
    • Aa
  • Access
  • Cited by 17
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Çakır, Özgür Meriç, Sinan and Arı, Şule 2016. Genetically Modified Organisms in Food.

    Lestingi, A. Facciolongo, A.M. Jambrenghi, A.Caputi Ragni, M. and Toteda, F. 2016. The use of peas and sweet lupin seeds alone or in association for fattening lambs: Effects on performance, blood parameters and meat quality. Small Ruminant Research, Vol. 143, p. 15.

    Calabrò, Serena Cutrignelli, Monica I Lo Presti, Vittorio Tudisco, Raffaella Chiofalo, Vincenzo Grossi, Micaela Infascelli, Federico and Chiofalo, Biagina 2015. Characterization and effect of year of harvest on the nutritional properties of three varieties of white lupine (Lupinus albusL.). Journal of the Science of Food and Agriculture, Vol. 95, Issue. 15, p. 3127.

    Matusiewicz, Magdalena Kosieradzka, Iwona Zuk, Magdalena and Szopa, Jan 2015. Effect of Dose and Administration Period of Seed Cake of Genetically Modified and Non-Modified Flax on Selected Antioxidative Activities in Rats. International Journal of Molecular Sciences, Vol. 16, Issue. 6, p. 14259.

    Rayan, Ahmed M. Nigussie, Fikru and Abbott, Louise C. 2015. Safety Evaluation of Stacked Genetically Modified Corn Event (MON89034 × MON88017) Using Zebrafish as an Animal Model. Food and Nutrition Sciences, Vol. 06, Issue. 14, p. 1285.

    Sirtori, Francesco Crovetti, Alessandro Acciaioli, Anna Bonelli, Antonio Pugliese, Carolina Bozzi, Riccardo Campodoni, Gustavo and Franci, Oreste 2015. Effect of Replacing a Soy Diet withVicia FabaandPisum Sativumon Performance, Meat and Fat Traits ofCinta SenesePigs. Italian Journal of Animal Science, Vol. 14, Issue. 1, p. 3659.

    Tudisco, R. Calabrò, S. Cutrignelli, M.I. Moniello, G. Grossi, M. Mastellone, V. Lombardi, P. Pero, M.E. and Infascelli, F. 2015. Genetically modified soybean in a goat diet: Influence on kid performance. Small Ruminant Research, Vol. 126, p. 67.

    Calabrò, S. Cutrignelli, M.I. Gonzalez, O.J. Chiofalo, B. Grossi, M. Tudisco, R. Panetta, C. and Infascelli, F. 2014. Meat quality of buffalo young bulls fed faba bean as protein source. Meat Science, Vol. 96, Issue. 1, p. 591.

    Cherdthong, A. Wanapat, M. Saenkamsorn, A. Waraphila, N. Khota, W. Rakwongrit, D. Anantasook, N. and Gunun, P. 2014. Effects of replacing soybean meal with dried rumen digesta on feed intake, digestibility of nutrients, rumen fermentation and nitrogen use efficiency in Thai cattle fed on rice straw. Livestock Science, Vol. 169, p. 71.

    El-Kholy, Thanaa Hilal, Mohammad Al-Abbadi, Hatim Serafi, Abdulhalim Al-Ghamdi, Ahmad Sobhy, Hanan and Richardson, John 2014. The Effect of Extra Virgin Olive Oil and Soybean on DNA, Cytogenicity and Some Antioxidant Enzymes in Rats. Nutrients, Vol. 6, Issue. 6, p. 2376.

    Hanaa, A. S. Oraby Mahrousa, M. H. Kandil Amal, A. M. Hassan and Hayam, A. Al-Sharawi 2014. Addressing the issue of horizontal gene transfer from a diet containing genetically modified components into rat tissues. African Journal of Biotechnology, Vol. 13, Issue. 48, p. 4410.

    Swiatkiewicz, Sylwester Swiatkiewicz, Malgorzata Arczewska-Wlosek, Anna and Jozefiak, Damian 2014. Genetically modified feeds and their effect on the metabolic parameters of food-producing animals: A review of recent studies. Animal Feed Science and Technology, Vol. 198, p. 1.

    Mastellone, Vincenzo Tudisco, Raffaella Monastra, Giovanni Pero, Maria Elena Calabrò, Serena Lombardi, Pietro Grossi, Micaela Cutrignelli, Monica Isabella Avallone, Luigi and Infascelli, Federico 2013. Gamma-Glutamyl Transferase Activity in Kids Born from Goats Fed Genetically Modified Soybean. Food and Nutrition Sciences, Vol. 04, Issue. 06, p. 50.

    Van Eenennaam, Alison L 2013. GMOs in animal agriculture: time to consider both costs and benefits in regulatory evaluations. Journal of Animal Science and Biotechnology, Vol. 4, Issue. 1, p. 37.

    Flachowsky, Gerhard Schafft, Helmut and Meyer, Ulrich 2012. Animal feeding studies for nutritional and safety assessments of feeds from genetically modified plants: a review. Journal für Verbraucherschutz und Lebensmittelsicherheit, Vol. 7, Issue. 3, p. 179.

    2012. Scientific Opinion on a request from the European Commission for the assessment of the scientific elements put forward by Hungary to support the prohibition for the placing on the market of GM potato EH92-527-1 for cultivation purposes in Hungary. EFSA Journal, Vol. 10, Issue. 12, p. 3021.

    Grønsberg, Idun M. Nordgård, Lise Fenton, Kristin Hegge, Beate Nielsen, Kaare M. Bardocz, Susan Pusztai, Arpad and Traavik, Terje 2011. Uptake and Organ Distribution of Feed Introduced Plasmid DNA in Growing or Pregnant Rats. Food and Nutrition Sciences, Vol. 02, Issue. 04, p. 377.


Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings

  • R. Tudisco (a1), V. Mastellone (a2), M. I. Cutrignelli (a1), P. Lombardi (a3), F. Bovera (a1), N. Mirabella (a3), G. Piccolo (a1), S. Calabrò (a1), L. Avallone (a3) and F. Infascelli (a1)
  • DOI:
  • Published online: 05 May 2010

The presence of DNA fragments in blood and milk from goats fed conventional (control) or Roundup Ready® soybean meal solvent extracted (s.e.; treated) was investigated by using a polymerase chain reaction approach. The same investigation was carried out on blood, skeletal muscle and organs from kids of both groups fed only dams’ milk until weaning. Moreover, the possible effects on cell metabolism were evaluated by determination of several specific enzymes in serum, heart, skeletal muscle, liver and kidney. Fragments of the multicopy chloroplast (trnL) gene were found in blood and milk samples from goats of both groups. In kids, the chloroplast fragments were found in samples of both groups. In samples, which proved positive for the presence of chloroplast DNA, fragments of the specific soybean single copy gene (lectin) were detected in several blood and milk samples. The same fragment was also found in control and treated groups of kids. Transgenic fragments were not found in those samples, which were found positive for chloroplast fragments of control groups of either goats or kids. On the contrary, in blood and milk of treated goats, fragments both of the 35S promoter and the CP4 epsps gene were detected. These fragments were also found in treated kids with a significant detection of the 35S promoter in liver, kidney and blood, and of the CP4 epsps gene fragment in liver, kidney, heart and muscle. A significant increase in lactic dehydrogenase, mainly concerning the lactic dehydrogenase-1 isoenzyme was found in heart, skeletal muscle and kidney of treated kids, thus suggesting a change in the local production of the enzyme. Finally, no significant differences were detected concerning kid body and organ weight.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the or variations. ‘’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings
      Available formats
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings
      Available formats
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings
      Available formats
Corresponding author
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

A Aumaitre , K Aulrich , A Chesson , G Flachowsky , G Piva 2002. New feeds from genetically modified plants: substantial equivalence, nutritional equivalence, digestibility, and safety for animals and the food chain. Livestock Production Science 74, 223238.

MT Bottero , T Civera , D Numera , S Rosati , P Sacchi , RM Turi 2002. A multiplex polymerase chain reaction for the identification of cow’s, goat’s and sheep’s milk in dairy products. International Dairy Journal 13, 277282.

P Cardarelli , MR Branquinho , RTB Ferreira , FP da Cruz , AL Gemal 2005. Detection of GMO in food products in Brazil: the INCQS experience. Food Control 16, 859866.

EH Chowdhury , H Kuribara , A Hino , P Sultana , O Mikami , N Shimada , KS Guruge , M Saito , Y Nakajiama 2003a. Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11. Journal of Animal Science 81, 25462551.

EFSA GMO Panel Working Group on Animal Feeding Trials2008. Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials. Food and Chemical Toxicology 46 (suppl. 1), S2S70.

R Einspanier , B Lutz , S Rief , O Berezina , V Zverlov , W Schwarz , J Mayer 2004. Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgene maize. European Food Research and Technology 218, 269273.

M Hernández , D Rodríguez-Lázaro , T Esteve , S Prat , M Pla 2003. Development of melting temperature-based SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection. Analytical Biochemistry 323, 164170.

JC Jennings , DC Kolwyck , SB Kats , AJ Whetsell , JB Surber , GL Cromwell , RP Lirette , KC Glenn 2003. Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal. Journal Animal Science 81, 14471455.

M Malatesta , C Caporaloni , S Gavaudan , MB Rocchi , S Serafini , C Tiberi , G Gazzanelli 2002. Ultrastructural morphometrical and immunocytochemical analyses of hepatocyte nuclei from mice fed on genetically modified soybean. Cell Structure and Function 27, 173180.

M Malatesta , F Boraldi , G Annovi , B Baldelli , S Battistelli , M Biggiogera , D Quaglino 2008. A long-term study on female mice fed on a genetically modified soybean: effects on liver ageing. Histochemistry and Cell Biology 130, 967977.

R Mazza , M Soave , M Morlacchini , G Piva , A Marocco 2005. Assessing the transfer of genetically modified DNA from feed to animal tissues. Transgenic Research 14, 775784.

A Nemeth , A Wurz , L Artim , S Charlton , G Dana , K Glenn , P Hunst , J Jennings , R Shilito , P Song 2004. Sensitive PCR analysis of animal tissue samples for fragments of endogenous and transgenic plant DNA. Journal of Agricultural and Food Chemistry 52, 61296135.

SR Padgette , KH Kolacz , X Delannay , DB Re , BJ LaVallee , CN Tinius , WK Rhodes , YI Otero , GF Barry , DA Eichholtz , VM Peschke , DL Nida , NB Taylor , GM Kishore 1995. Development, identification and characterization of a glyphosate-tolerant soybean line. Crop Science 35, 14511461.

RH Phipps , ER Deaville , BC Maddison 2003. Detection of transgenic and endogenous plant DNA in rumen fluid, duodenal digesta, milk, blood, and feces of lactating dairy cows. Journal of Dairy Science 86, 40704078.

A Rizzi , L Brusetti , S Arioli , KM Nielsen , I Tamagnini , A Tamburini , C Sorlini , D Daffonchio 2008. Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria. European Food Research and Technology 227, 16991709.

R Sharma , D Damgaard , TW Alexander , MER Dugan , JL Aalhus , K Stanford , TA McAllister 2006. Detection of transgenic and endogenous plant DNA in digesta and tissues of sheep and pigs fed Roundup Ready canola meal. Journal of Agricultural Food Chemistry 54, 16991709.

M Singh , DP Tiwari , A Kumar , M Ravi Kumar 2003. Effect of feeding transgenic cottonseed vis-a-vis non-transgenic cottonseed on haematobiochemical constituents in lactating Murrah buffaloes. Asian–Australian Journal of Animal Science 16, 17321737.

V Terzi , F Infascelli , R Tudisco , G Russo , AM Stanca , P Faccioli 2004. Quantitative detection of Secale cereale by real-time PCR amplification. LWT-Food Science and Technology 37, 239246.

MA Tony , A Butschke , H Broll , L Grohmann , J Zagon , I Halle , S Dänicke , M Schauzu , HM Hafez , G Flachowsky 2003. Safety assessment of Bt 176 maize in broiler nutrition: degradation of maize-DNA and its metabolic fate. Archives of Animal Nutrition 57, 235252.

R Tudisco , F Infascelli , MI Cutrignelli , F Bovera , C Morcia , P Faccioli , V Terzi 2006a. Fate of feed plant DNA monitored in water buffalo (Bubalus bubalis) and rabbit (Oryctolagus cuniculus). Livestock Science 105, 1218.

G Van Hall 2000. Lactate as a fuel for mitochondrial respiration. Acta Physiologica Scandinava 168, 643656.

PJ Van Soest , JB Robertson , BA Lewis 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 35833598.

C Yonemochi , T Ikeda , C Harada , T Kusama , M Hanazumi 2003. Influence of transgenic corn (CBH 351, named Starlink) on health condition of dairy cows and transfer of Cry9C protein and cry9C gene to milk, blood, liver and muscle. Animal Science Journal 74, 8188.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 1751-7311
  • EISSN: 1751-732X
  • URL: /core/journals/animal
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Type Description Title
Supplementary Materials

Tudisco supplementary material

 Unknown (50 KB)
50 KB